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The map label for this gene is dsbA [H]

Identifier: 126174853

GI number: 126174853

Start: 3098219

End: 3098839

Strand: Reverse

Name: dsbA [H]

Synonym: Sbal_2640

Alternate gene names: 126174853

Gene position: 3098839-3098219 (Counterclockwise)

Preceding gene: 126174854

Following gene: 126174852

Centisome position: 60.44

GC content: 49.92

Gene sequence:

>621_bases
ATGAGCGTGTTTAATCGAGTTCGACCTATAGCCGCATTGGCGTGCGGATTAATGCTGAGTGCCAGCTTTGCACTGCAGGC
CGCAAGTTTCGAAGAAGGCAAAGATTACGTCACAGTGGCGGGGATTACCGAAGCGCAAAAGCCAGTGCTGCGAGAGTTTT
TCTCCTATAACTGCCCACATTGCTATAAGCAAGAACCGCTTATGGACTTGACAGTGCAACTGCTCGGCAAAGACGTGGCT
TTTGAGCGTACCCCTGTGGGCGCCGGCCGTCCTGCTTGGGAGCTGAGTCAGCTTGCCTACTATGTGGCGCAAAAATTAAA
GATGACTAAGCAAACCCATGGCGCTATTTTCAAGCAAATCCATGAGAAAGGTGAGCAATTTACAGCACCTGAACAGGTTA
AAGCCTTCTTTGTCGCCCAAGGCGCTAAGGTGGATGATGTCGATGCCGCAATGAATTCCGTCGATGCCAAATTCACCCTA
ATGAATTATGACTCTCAAGCTGAGCTTGCAGGGATCAAGGGCGTACCCTCATTACTGGTGAATGGCCGTTATATGCTGAC
GTCGACCGCGCACACGCCAGAAGAGTTAGCTGCATTAGTTAAGTTTTTAGCCGCGAAGTAA

Upstream 100 bases:

>100_bases
GTAAAAGCCGAACTTTTAGGTTATCTTTGGGTTCCAAGTTTTGCTTGAGCGAATGTGTCATCCTTATTTAAACCAATGAC
GAATAAAAAGAGACAATATA

Downstream 100 bases:

>100_bases
CCGCGTTTAATAAATTGCGACTTGACTCATCGCGGCTAACGAATAAGCGCCTTGCGAGCTGCATTGGGAAACTTATCCCG
CTATTCGCTAATCTAGCGTT

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSVFNRVRPIAALACGLMLSASFALQAASFEEGKDYVTVAGITEAQKPVLREFFSYNCPHCYKQEPLMDLTVQLLGKDVA
FERTPVGAGRPAWELSQLAYYVAQKLKMTKQTHGAIFKQIHEKGEQFTAPEQVKAFFVAQGAKVDDVDAAMNSVDAKFTL
MNYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK

Sequences:

>Translated_206_residues
MSVFNRVRPIAALACGLMLSASFALQAASFEEGKDYVTVAGITEAQKPVLREFFSYNCPHCYKQEPLMDLTVQLLGKDVA
FERTPVGAGRPAWELSQLAYYVAQKLKMTKQTHGAIFKQIHEKGEQFTAPEQVKAFFVAQGAKVDDVDAAMNSVDAKFTL
MNYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK
>Mature_205_residues
SVFNRVRPIAALACGLMLSASFALQAASFEEGKDYVTVAGITEAQKPVLREFFSYNCPHCYKQEPLMDLTVQLLGKDVAF
ERTPVGAGRPAWELSQLAYYVAQKLKMTKQTHGAIFKQIHEKGEQFTAPEQVKAFFVAQGAKVDDVDAAMNSVDAKFTLM
NYDSQAELAGIKGVPSLLVNGRYMLTSTAHTPEELAALVKFLAAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=212, Percent_Identity=27.8301886792453, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22573; Mature: 22442

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFNRVRPIAALACGLMLSASFALQAASFEEGKDYVTVAGITEAQKPVLREFFSYNCPH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
CYKQEPLMDLTVQLLGKDVAFERTPVGAGRPAWELSQLAYYVAQKLKMTKQTHGAIFKQI
CCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKGEQFTAPEQVKAFFVAQGAKVDDVDAAMNSVDAKFTLMNYDSQAELAGIKGVPSLLV
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCHHHHH
NGRYMLTSTAHTPEELAALVKFLAAK
CCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVFNRVRPIAALACGLMLSASFALQAASFEEGKDYVTVAGITEAQKPVLREFFSYNCPH
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCC
CYKQEPLMDLTVQLLGKDVAFERTPVGAGRPAWELSQLAYYVAQKLKMTKQTHGAIFKQI
CCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKGEQFTAPEQVKAFFVAQGAKVDDVDAAMNSVDAKFTLMNYDSQAELAGIKGVPSLLV
HHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCHHHHH
NGRYMLTSTAHTPEELAALVKFLAAK
CCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA