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The map label for this gene is topA [H]

Identifier: 126174709

GI number: 126174709

Start: 2915280

End: 2917922

Strand: Reverse

Name: topA [H]

Synonym: Sbal_2496

Alternate gene names: 126174709

Gene position: 2917922-2915280 (Counterclockwise)

Preceding gene: 126174711

Following gene: 126174707

Centisome position: 56.91

GC content: 47.52

Gene sequence:

>2643_bases
ATGGGTAAATCGCTAGTTATTGTCGAATCACCGGCCAAAGCTAAGACTATTAATAAATATCTTGGCAAAGAATTCATCGT
TAAATCGAGCGTAGGTCACATCCGTGATCTGCCGACATCATCGACTTCAGAAGGCAATGAAAAGGTTAAATCCGCTGCCG
AAGTTAAGAAAATGTCGCCAGAAGAAAAAGCTCAATATAAAAAGGTGAAAGATCAGCAAGCGCTGGTGTCTCGCATGGGC
GTAAACCCTGAAAAGGGCTGGGCAGCTAAGTATCAAATTTTGCCGGGCAAAGAAAAGGTCGTTAAAGAGTTACAGGCACT
TGCCGATTCTGCCGACCAAATCTATCTTGCAACCGACTTAGACCGTGAGGGAGAAGCTATCGCCTGGCATTTGCAAGAGG
TGATTGGCGGGGATCCTTCACGGTATCAGCGTGTGGTTTTCAACGAAATTACTAAGTCTGCGATTCAAGACGCTTTTAGC
AAGCCGTCGACACTCGACACTAACATGGTTAATGCCCAGCAAGCTCGTCGCTTCTTAGACAGAGTTGTCGGTTTTATGGT
GTCGCCACTGCTGTGGAAAAAAGTCGCTCGCGGTTTATCCGCTGGACGAGTACAGTCGGTTGCCGTTCGCTTAGTGGTTG
AACGTGAAAGCGAAATTAAAGCCTTTGTGCCGGAAGAGTTCTGGGATGTACATGCAGAGCTGAGTACGCCCGCTCAAGAA
GCCTTGCGGATGGAAGTGGTTAAGCATTTAGATGCGGCCTTTAATCCTATCAATGAACAGCAAGCGATGGCGGCGGTTCA
AGCGTTATCCGCGGCGAGTTTTACCGTGCTGGCCCGCGAAGACAAAGCGACCCAAAGCAAACCTTCTGCGCCTTTTATTA
CATCGACATTGCAACAGGCGGCGAGTACGCGCTTAGGTTTCGGTGTGAAGAAGACCATGATGATGGCTCAGCGACTTTAT
GAAGCTGGTCATATCACTTATATGCGTACCGACTCGACCAATTTGAGTCAAGAAGCCGTTGAAAACTTGCGTGATATGAT
TGCCAAAGAGTTTGGTGATAAATATCTACCCGCGGAACCCATTCGCTACGGTTCTAAGGAAGGCGCACAAGAGGCTCACG
AAGCGATTCGTCCATCGAACGTTCATGTACAAGCGGCGACCTTATCGGACATGGAGCGTGATGCTCAGCGTCTATATGAG
CTGATTTGGCGTCAATTTGTGTCGTGTCAGATGACGCCAGCCCAATATGATGCTACTAAGTTGACCGTAAAAGCGGGTGA
CTATGAGCTGAAGGCTACTGGACGTACTTTACGTTTTGATGGTTGGACTCGCGTGCAAACAGCACTTAAGAAGAAAAACG
AAGAAGACAATACGTTGCCATTTGTGGCACAAGGTGATGTATTAGCGTTAAAAGAGTTGCAACCTAAGCAACACTTTACT
AAGCCGCCAGCTCGATACAGCGAAGCCTCTTTAGTTAAAGAATTAGAAAAACGCGGCATTGGCCGCCCATCGACCTACGC
AACTATTATTTCAACGATTCAAGATCGGGGTTATGTAAAAGTTGAGAATCGTCGTTTCTATGCCGAAAAAATGGGTGAAA
TTGTCAGCGAGCGATTAGTCGGCAGTTTTGCGGATTTAATGAGCTATGACTTTACCGCCAGTATGGAGCAAACATTAGAC
GATGTGGCTCGCGGCGAATTAGATTGGAAAAAAGTGCTCGATGGCTTCTATGCTGACTTTACTAAGCAATTAGAAAAAGC
GGAGCTGGATCCCGAAGAAGGTGGCATGCGTCTTAACCAAATGGTGATGACGGATATCAAGTGCCCAACCTGTGGTCGCT
CTATGGGTATTCGTACAGGTACGACTGGTGTTTTCCTTGGTTGCTCTGGTTACGAATTACCTCCCAAAGAGCGCTGTAAG
ACAACGATGAACCTTACGCCTGGCGTTGAGGCGATTAGCGAAAACAGTGAAGATGCCGAGACTGATGCACTGCGTGCGAA
GCACCGCTGTAGTATTTGCGGCACAGCAATGGACAGTTACCTAATTGATGAGAAGCGTAAGCTACACGTCTGTGGTAACA
ACCCGATTTGTGAAGGCTATGAAGTCGAAGAAGGCCAATTCAAGATCAAGGGCTATGAAGGTCCGCTGATTGAGTGTGAT
CGCTGTGGCCACGACATGGAACTGAAAAACGGCCGTTTTGGTAAGTATTTTGGTTGTACCAATAGCGAATGTAAAAATAC
GCGTAAATTACTGAAGAACGGCGAGGCTGCGCCACCAAAAGAAGATCCTATCTATTTGCCTGATCTGAAATGCAGCAAAT
CGGATGCCCATTTCGTATTACGTGATGGTGCAGCAGGGATCTTCTTAGCGGCCAGTACCTTCCCTAAATCACGGGAAACC
CGTGCACCTCTGGTTGAAGAGCTGGTTCAATACCGCGAGCTGCTGTGGCCTAAGTATCAATATTTAGCTGATGCCCCCGT
CACTGATGACGAAGGCAATAAAGCAGCGGTGAAGTTTAGTCGTAAGACGAAAGAGCAATACGTTGCGACTGAAGTCGACG
GCAAAGCCACTGGTTGGTCAGCGCATTACGTTAATGGCAAGTGGGTGAGTGAGACTACCAAGAAAGCCAAGAAAAGCGCA
TAG

Upstream 100 bases:

>100_bases
GGTACATATAAAAGAGGGATTAACTTGAAACTATCAGTACAACCCTCTATATATGGTGCCAATTTCCACCTTCTTGAGAT
TTTTTGGCGCAAATTAAACT

Downstream 100 bases:

>100_bases
CGGCGGATTAAGCTAGCTTAACGCCAAGAGAATAACAAAAAAGCCAAGTCAATTATGACTTGGCTTTTTTGTTTCGATTT
TCTTTAGAGACTGATTTGTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMG
VNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFS
KPSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAELSTPAQE
ALRMEVVKHLDAAFNPINEQQAMAAVQALSAASFTVLAREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLY
EAGHITYMRTDSTNLSQEAVENLRDMIAKEFGDKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVQAATLSDMERDAQRLYE
LIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTALKKKNEEDNTLPFVAQGDVLALKELQPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVGSFADLMSYDFTASMEQTLD
DVARGELDWKKVLDGFYADFTKQLEKAELDPEEGGMRLNQMVMTDIKCPTCGRSMGIRTGTTGVFLGCSGYELPPKERCK
TTMNLTPGVEAISENSEDAETDALRAKHRCSICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
RCGHDMELKNGRFGKYFGCTNSECKNTRKLLKNGEAAPPKEDPIYLPDLKCSKSDAHFVLRDGAAGIFLAASTFPKSRET
RAPLVEELVQYRELLWPKYQYLADAPVTDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVNGKWVSETTKKAKKSA

Sequences:

>Translated_880_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMG
VNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFS
KPSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAELSTPAQE
ALRMEVVKHLDAAFNPINEQQAMAAVQALSAASFTVLAREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLY
EAGHITYMRTDSTNLSQEAVENLRDMIAKEFGDKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVQAATLSDMERDAQRLYE
LIWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTALKKKNEEDNTLPFVAQGDVLALKELQPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVGSFADLMSYDFTASMEQTLD
DVARGELDWKKVLDGFYADFTKQLEKAELDPEEGGMRLNQMVMTDIKCPTCGRSMGIRTGTTGVFLGCSGYELPPKERCK
TTMNLTPGVEAISENSEDAETDALRAKHRCSICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
RCGHDMELKNGRFGKYFGCTNSECKNTRKLLKNGEAAPPKEDPIYLPDLKCSKSDAHFVLRDGAAGIFLAASTFPKSRET
RAPLVEELVQYRELLWPKYQYLADAPVTDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVNGKWVSETTKKAKKSA
>Mature_879_residues
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSPEEKAQYKKVKDQQALVSRMGV
NPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDLDREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSK
PSTLDTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAELSTPAQEA
LRMEVVKHLDAAFNPINEQQAMAAVQALSAASFTVLAREDKATQSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYE
AGHITYMRTDSTNLSQEAVENLRDMIAKEFGDKYLPAEPIRYGSKEGAQEAHEAIRPSNVHVQAATLSDMERDAQRLYEL
IWRQFVSCQMTPAQYDATKLTVKAGDYELKATGRTLRFDGWTRVQTALKKKNEEDNTLPFVAQGDVLALKELQPKQHFTK
PPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLVGSFADLMSYDFTASMEQTLDD
VARGELDWKKVLDGFYADFTKQLEKAELDPEEGGMRLNQMVMTDIKCPTCGRSMGIRTGTTGVFLGCSGYELPPKERCKT
TMNLTPGVEAISENSEDAETDALRAKHRCSICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECDR
CGHDMELKNGRFGKYFGCTNSECKNTRKLLKNGEAAPPKEDPIYLPDLKCSKSDAHFVLRDGAAGIFLAASTFPKSRETR
APLVEELVQYRELLWPKYQYLADAPVTDDEGNKAAVKFSRKTKEQYVATEVDGKATGWSAHYVNGKWVSETTKKAKKSA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=507, Percent_Identity=27.4161735700197, Blast_Score=155, Evalue=2e-37,
Organism=Homo sapiens, GI10835218, Length=624, Percent_Identity=25, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=71.4121699196326, Blast_Score=1296, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=565, Percent_Identity=24.4247787610619, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32563869, Length=598, Percent_Identity=24.9163879598662, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=26.1208576998051, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640096, Length=506, Percent_Identity=27.0750988142292, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24585251, Length=600, Percent_Identity=23.3333333333333, Blast_Score=116, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98235; Mature: 98104

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSP
CCCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCH
EEKAQYKKVKDQQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSKPSTLDTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAELSTPAQE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCHHHHCCCHHHHHHHHHCCCCHHH
ALRMEVVKHLDAAFNPINEQQAMAAVQALSAASFTVLAREDKATQSKPSAPFITSTLQQA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEAVENLRDMIAKEFGDKYLPAEP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVHVQAATLSDMERDAQRLYELIWRQFVSCQMTPAQYDATK
CCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LTVKAGDYELKATGRTLRFDGWTRVQTALKKKNEEDNTLPFVAQGDVLALKELQPKQHFT
EEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCCCCCEEECCCEEEHHHCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
GSFADLMSYDFTASMEQTLDDVARGELDWKKVLDGFYADFTKQLEKAELDPEEGGMRLNQ
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
MVMTDIKCPTCGRSMGIRTGTTGVFLGCSGYELPPKERCKTTMNLTPGVEAISENSEDAE
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECCCCCCHHHHCCCCCCHH
TDALRAKHRCSICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCEEEEECCCCCEEEHH
RCGHDMELKNGRFGKYFGCTNSECKNTRKLLKNGEAAPPKEDPIYLPDLKCSKSDAHFVL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCEEE
RDGAAGIFLAASTFPKSRETRAPLVEELVQYRELLWPKYQYLADAPVTDDEGNKAAVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEH
RKTKEQYVATEVDGKATGWSAHYVNGKWVSETTKKAKKSA
HHHHHHHHHEECCCCCCCCEEEECCCEEHHHHHHHHHCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSTSEGNEKVKSAAEVKKMSP
CCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCH
EEKAQYKKVKDQQALVSRMGVNPEKGWAAKYQILPGKEKVVKELQALADSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDPSRYQRVVFNEITKSAIQDAFSKPSTLDTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVERESEIKAFVPEEFWDVHAELSTPAQE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCHHHHCCCHHHHHHHHHCCCCHHH
ALRMEVVKHLDAAFNPINEQQAMAAVQALSAASFTVLAREDKATQSKPSAPFITSTLQQA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQEAVENLRDMIAKEFGDKYLPAEP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVHVQAATLSDMERDAQRLYELIWRQFVSCQMTPAQYDATK
CCCCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
LTVKAGDYELKATGRTLRFDGWTRVQTALKKKNEEDNTLPFVAQGDVLALKELQPKQHFT
EEEEECCEEEEECCCEEEECCHHHHHHHHHHCCCCCCCCCEEECCCEEEHHHCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYATIISTIQDRGYVKVENRRFYAEKMGEIVSERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
GSFADLMSYDFTASMEQTLDDVARGELDWKKVLDGFYADFTKQLEKAELDPEEGGMRLNQ
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
MVMTDIKCPTCGRSMGIRTGTTGVFLGCSGYELPPKERCKTTMNLTPGVEAISENSEDAE
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCEECCCCCCHHHHCCCCCCHH
TDALRAKHRCSICGTAMDSYLIDEKRKLHVCGNNPICEGYEVEEGQFKIKGYEGPLIECD
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCEEEEECCCCCEEEHH
RCGHDMELKNGRFGKYFGCTNSECKNTRKLLKNGEAAPPKEDPIYLPDLKCSKSDAHFVL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCEEE
RDGAAGIFLAASTFPKSRETRAPLVEELVQYRELLWPKYQYLADAPVTDDEGNKAAVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEEH
RKTKEQYVATEVDGKATGWSAHYVNGKWVSETTKKAKKSA
HHHHHHHHHEECCCCCCCCEEEECCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]