Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 126174671

GI number: 126174671

Start: 2871490

End: 2872446

Strand: Reverse

Name: holB [H]

Synonym: Sbal_2458

Alternate gene names: 126174671

Gene position: 2872446-2871490 (Counterclockwise)

Preceding gene: 126174672

Following gene: 126174670

Centisome position: 56.02

GC content: 47.86

Gene sequence:

>957_bases
ATGGTAGTTGAGTCTGGGTTTACGGCTGCGCAATTGCCTTGGCTCGAAGGGCCAAGGCAGGCATTTTTAGCGCAACTAAC
GCGGCAAAAAATGCCCCATGCGCAGCTTGTCGGCATTGATTCTGCCTATGGTGGTGAGCTGCTGAGTGTATTTATGGCGC
GTGCGGCCATGTGTTTGCAGCCAACACCGGCGGGGGCCTGCGGTTTTTGTAAGTCTTGTCAGCTTTTTGATGCGGGCAAT
CATCCCGATTTTTACCATATTGCTGCCGATGGCAATCAAATTAAAGTCGATCAAATTCGTGAGCTTTGCACGCGTCTTAC
TGCTACAGCCCAGCAAAGCGGTATGCGAGTGGCGATTATTCATCATAGCGAGCGGCTTAACTCCGCTGCCGCCAACGCTC
TCCTTAAAACCCTTGAGGAGCCGGGTAAAGACACTTTACTGATATTGCAGTCGGATACGCCTGCACGTTTAATGGCGACG
GTCGGCAGTCGTTGTCAGCGCATTCCCTTCGTTGTGCCAAGTCGAGAAATCATCAAAAACTGGTTAGCGCAGCAATGTGA
TATCAAAGAGGATGTTACTTGGTGCTTGAGTGTGGTGGGTGGACCGCTACAGTTGGCAGAGAGCTTGCAGTCTGAAGGTG
GTCAACAGAGCCGTTATCAATCCTTGCTACAGTTTCGCAAAGATTGGGCCCAAAGTTTGTCGTCTGGTCATCTGTGTGCT
AGTTTGTTAACTATAAGTGAACAACAAATAATTGATGTTCTTAAGGTTTTATACCTGCTTATACGACAGGTGTTACTGAA
AAATAGCCACCAAGATGCGTTTATCCATGCGCAGCTCGGGAACTTGGCGGCCAAGATCATGGGAATGTGTCATAGCTTAA
GTACAATGCCGAGCGTCAATTATCTGGCCTTGTGTCAAAGTTATGTCTTAGAATATAGAGAGATAACAAATAAGTAA

Upstream 100 bases:

>100_bases
TTAAACTTGCTAGCGAAGATGACAGCATAGTGGTTATCGATGCGAGTCAAACCATGGCCGAAGTGCATAAAGATATCTTA
GCCGTGTTACAGGCGATGGC

Downstream 100 bases:

>100_bases
TATGCCATGGTAGATCTCGTCGTTAATTTTGATACCTTACATCAGCTATATCGTGCCTATATGCCTTTTATTAAGCCGGC
AGGATTATTCATAGCCACCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVVESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTPAGACGFCKSCQLFDAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VGSRCQRIPFVVPSREIIKNWLAQQCDIKEDVTWCLSVVGGPLQLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK

Sequences:

>Translated_318_residues
MVVESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTPAGACGFCKSCQLFDAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VGSRCQRIPFVVPSREIIKNWLAQQCDIKEDVTWCLSVVGGPLQLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK
>Mature_318_residues
MVVESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQPTPAGACGFCKSCQLFDAGN
HPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAIIHHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMAT
VGSRCQRIPFVVPSREIIKNWLAQQCDIKEDVTWCLSVVGGPLQLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVNYLALCQSYVLEYREITNK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=34.8066298342541, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=28.6764705882353, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35055; Mature: 35055

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQ
CEECCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPAGACGFCKSCQLFDAGNHPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAII
CCCCCHHHHHCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMATVGSRCQRIPFVVPSREIIKN
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
WLAQQCDIKEDVTWCLSVVGGPLQLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLALCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVVESGFTAAQLPWLEGPRQAFLAQLTRQKMPHAQLVGIDSAYGGELLSVFMARAAMCLQ
CEECCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPAGACGFCKSCQLFDAGNHPDFYHIAADGNQIKVDQIRELCTRLTATAQQSGMRVAII
CCCCCHHHHHCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSAAANALLKTLEEPGKDTLLILQSDTPARLMATVGSRCQRIPFVVPSREIIKN
ECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
WLAQQCDIKEDVTWCLSVVGGPLQLAESLQSEGGQQSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDVLKVLYLLIRQVLLKNSHQDAFIHAQLGNLAAKIMGMCHSLSTMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLALCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]