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The map label for this gene is purR [H]

Identifier: 126174569

GI number: 126174569

Start: 2742750

End: 2743796

Strand: Reverse

Name: purR [H]

Synonym: Sbal_2356

Alternate gene names: 126174569

Gene position: 2743796-2742750 (Counterclockwise)

Preceding gene: 126174570

Following gene: 126174567

Centisome position: 53.51

GC content: 48.04

Gene sequence:

>1047_bases
ATGGCATCGAAAGCGACCTCGATTGATATTGCTTATCGTGCTGGCGTATCCCAGTCTACGGTTTCCCGTGCCCTTAGAAA
CAGTCCGTTAGTCAATTTAGAGACCCGTCAACGTATTCAAGCCATTGCTAAAGAGCTGAATTACAAGGTTGATAAAAACG
CCAGTAATCTTCGCACCCAAAATAGCCATACCTTAGCGCTGTTATTGTGTGAAGACCCCACCAATGATGACTCGTTAATC
AATCCCTTTTTCCTGTCTATGCTGGGTTCCATTACCCGAGCAACGGCGCAGCAGGGTTATGATTTACTCGTTTCTTTTCA
GCAATTATCCAGCGATTGGCATGCCGATTATGAAGACAGCAATAAAGCCGATGGCATTATTCTGCTCGGTTATGGCGACT
ATATGGATTACGAGCAAAAGCTGGAGCGACTGTTAGCGCAGAATACCCATTTTGTGATTTGGGGCGCCGAGCATAATAAT
AAGTCTGTGCTATCGATTGGCTGTGATAATCATCAAGGCGGCTTGATTGCGACAGAGCATTTAATTTCCCTTGGCCGCAA
TGCTTTTGCCTTTTTAGGTGATGCTTCGAGCCATAGCCCTGAGTTTAGGGACAGATATTTAGGGCATGTGAAGGCGCTGC
AAACGGCGAATATTCCTGTGGATCGCACTAAGCAAGTGTCGGCCATCAGCACAGAAAGTTCAGGCTACGATGCCGCTATG
GTGTTGATTGCCCAAGGCGCTAAGTTTGATGCGATATTTGCGGCCAGCGATTTGATCGCCATCGGCGCGATTCGGGCGCT
GAAAGAAAAGGGCATTTCAGTCCCAGAGCAAGTGGCCGTTGTAGGATATGACGATATTCCGGTGGCGAGTTTTGCTAATC
CGCCATTAACGACCATTAAGCAGAACACCCAATTAGCCGGTGAGATCTTAGTCGACAGTGTACTCAAACTTATCCGCGGA
CAAGAGGTGAGTGCGCAGCTTATTCCGACAACCTTAGTGGTTCGCAGCTCCTGCGGCATTGATAGTGCGCCGCTTAAGCC
GTTATAG

Upstream 100 bases:

>100_bases
AAATTCAAGTGTAAGACGGATGTTAACGCATTCGTCTATACAAGTTAGCGGAACTGTTTTAGTATCGACGCAAAATAATC
ACTAAGGTTACCTCCTCTCT

Downstream 100 bases:

>100_bases
ATGGCCGTTATCGCTAGGCGTTTGTAGCTCTATCATACTGCTATTTGTTGGCAACTCGTTGGTCACTTAATTTACCGATT
GAAACAACGCCGATAATCCT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRNAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGISVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRG
QEVSAQLIPTTLVVRSSCGIDSAPLKPL

Sequences:

>Translated_348_residues
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLI
NPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNN
KSVLSIGCDNHQGGLIATEHLISLGRNAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGISVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRG
QEVSAQLIPTTLVVRSSCGIDSAPLKPL
>Mature_347_residues
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQNSHTLALLLCEDPTNDDSLIN
PFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDSNKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNK
SVLSIGCDNHQGGLIATEHLISLGRNAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAMV
LIAQGAKFDAIFAASDLIAIGAIRALKEKGISVPEQVAVVGYDDIPVASFANPPLTTIKQNTQLAGEILVDSVLKLIRGQ
EVSAQLIPTTLVVRSSCGIDSAPLKPL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=28.1437125748503, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=29.080118694362, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=27.2727272727273, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=28.9036544850498, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=31.4705882352941, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=352, Percent_Identity=26.9886363636364, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=27.4834437086093, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=27.0348837209302, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=29.0909090909091, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790715, Length=299, Percent_Identity=26.7558528428094, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=23.8532110091743, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787906, Length=349, Percent_Identity=24.6418338108883, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790689, Length=342, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37715; Mature: 37584

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRNAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEE
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGISVPEQVAVVGYDDIPVASFANPPLTTIK
EEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
QNTQLAGEILVDSVLKLIRGQEVSAQLIPTTLVVRSSCGIDSAPLKPL
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ASKATSIDIAYRAGVSQSTVSRALRNSPLVNLETRQRIQAIAKELNYKVDKNASNLRTQ
CCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCEECCCCHHCCCC
NSHTLALLLCEDPTNDDSLINPFFLSMLGSITRATAQQGYDLLVSFQQLSSDWHADYEDS
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
NKADGIILLGYGDYMDYEQKLERLLAQNTHFVIWGAEHNNKSVLSIGCDNHQGGLIATEH
CCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHH
LISLGRNAFAFLGDASSHSPEFRDRYLGHVKALQTANIPVDRTKQVSAISTESSGYDAAM
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEE
VLIAQGAKFDAIFAASDLIAIGAIRALKEKGISVPEQVAVVGYDDIPVASFANPPLTTIK
EEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
QNTQLAGEILVDSVLKLIRGQEVSAQLIPTTLVVRSSCGIDSAPLKPL
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA