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The map label for this gene is gloA [H]

Identifier: 126174485

GI number: 126174485

Start: 2626375

End: 2626785

Strand: Direct

Name: gloA [H]

Synonym: Sbal_2269

Alternate gene names: 126174485

Gene position: 2626375-2626785 (Clockwise)

Preceding gene: 126174484

Following gene: 126174488

Centisome position: 51.22

GC content: 46.96

Gene sequence:

>411_bases
ATGTCGCAACTATTACACACTATGATCCGCGTCGGTAACTTAGAACGCAGTATTGCTTTTTATACTGAAGTGCTCGGCAT
GAAACTCCTTCGCACCTCAGAAAACGCAGAATACAAATATTCTTTAGCTTTCGTAGGTTACGGTGAAGAGTCCACTGGCC
AAGCCGTTGTTGAACTGACCTATAACTGGGGCACAGACAAATACGATTTAGGCACAGGCTTTGGCCACTTAGCCATTGGC
GATGAAGATATTTACGCCCGTTGTGCTGCAATTGCTGCAGCCGGTGGCAAAGTAACACGCGCCCCAGGTCCAGTCGCAGG
CGGCACCACTGAGATTGCCTTTGTTGAAGATCCAGACGGTTATAAAATTGAGTTCATCCAAATGAAATCGGCGACTCAAG
GTTTAGGTTAA

Upstream 100 bases:

>100_bases
TTGGTCAAGTTCGATAAAAACACAATGGATGACATTTGTAGTCATACGCACATACATTTTTGATATACTCCTCGCCGTAA
TCTATTTATAGAGAGGACCT

Downstream 100 bases:

>100_bases
GTCTTGCCACCTGAGCAGAACAGTTCGCTAAATGAGAAATGCCGAATCTTGATTCGGCATTTTTGTTTTTATAATTTATA
TCTTGCGTTAGGCACAAAGA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENAEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIG
DEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Sequences:

>Translated_136_residues
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENAEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIG
DEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG
>Mature_135_residues
SQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENAEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIGD
EDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=32.6530612244898, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=68.6567164179104, Blast_Score=187, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=42.8571428571429, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=34.8993288590604, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=32.3741007194245, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14626; Mature: 14494

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENAEYKYSLAFVGYGEESTGQAVVELT
CHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTDKYDLGTGFGHLAIGDEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC
>Mature Secondary Structure 
SQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENAEYKYSLAFVGYGEESTGQAVVELT
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTDKYDLGTGFGHLAIGDEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]