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The map label for this gene is norM [H]

Identifier: 126174361

GI number: 126174361

Start: 2489434

End: 2490813

Strand: Reverse

Name: norM [H]

Synonym: Sbal_2144

Alternate gene names: 126174361

Gene position: 2490813-2489434 (Counterclockwise)

Preceding gene: 126174363

Following gene: 126174360

Centisome position: 48.58

GC content: 47.68

Gene sequence:

>1380_bases
ATGAATAATTCAGGCTATCAAGCCAAACGTTTAATCCAATTGGCGCTGCCGGTGCTAATCGCTCAGGTCACTCAGACCAT
GATGGGCTTTATTGATACCGTTATGGCGGGCCGTGTTAGCGCGGTTGATATGGCCGCTGTTGCGGTTGGCACTAGCCTTT
GGTTGCCCGCTATTCTGTTCGTCCAAGGTTTACTGATGGCTTTTACGCCTTTATTTGCCCACTACAATGGAGCGAATAAT
CAAAAAGCCATCCAACCTTTGGCCTTTCAAGGTGCCTATCTTGCCATCATTGGTGGCATTGGCGTGATGATTTTCCTCGC
ATCAGCGCCCTTTGTGCTTGGCCATATGGATTTAGAACCCGAATTATACCGCTTAACCATTGGATATCTCGACGGTATTT
TATGGGGTGCGCCCGCATTTGTGCTTTATCAAGTGCTGCGCGGCTGTAGTGAAGGGATTTCTTACACGTTACCGACTATG
GTCATTGGCTTTGTCGGCTTAGCAGTGAACATCCCCGCCAACTACATCTTTATTTATGGACACTTTGGCGTACCTCCGAT
GGGCGGTGCTGGCTGTGGTATTGCCACCGCTTTGGTTTTTTGGGCCATGCTGATTGCTATGACTATTTATATGCAACTGC
ATAAGAAATTCGCGGCCTTGGCTCCTTTCAGTAAATTTCATCGTCCTGACTTTGAAACGATGAAAAAAATGACCAAACTC
GGCATGCCGATAGCAATGGCGCTGTTTTTTGAAGTGAGTTTATTTGCCATTATCGCGCTGTTACTCGCCCCATTAGGCGC
AACTGTGGTGGCAAGTCACCAGATAGCACTGAACTTTTCTGCCATCGTATTTATGCTGCCGTTATCGATTGGTATCGCGG
TATCGATTCGAATTGGTTACTACTTAGGCCGGGATCGCGCCGATATTTCCGCAGTGGTTACCAAAGTGGGTTTATGGCTG
GCGCTGTCCCTTGCATTATCAACTGCAGTCTTGACCGTCATATTTAGATTCCAGATTGCAGAGCTTTATAATGGTGATCC
TGAAGTTGTCGCTTTAGCTGGCAGCTTGATGTTAATGGCGGCACTGTATCAATTATCGGATTCAGTCCAAGTGGTTGCCG
CCGGAGCCCTACGTGGTTATAAAGATACCCGCAGTGCCTTTTATATCACGCTGTTTTCCTATTGGGGCATAGGGATGTCA
CTCGGTTATACCTTGGCTTATACGGATCATATCGTTCCTGCCATGGGCGCCCATGGTTTCTGGGCAGGATTGATTGCAGG
TCTCACCTCGGCGGCGCTGCTATTTTTCATTCGACTGCGTTATATCCAAAAACATGGCGTGCATTTAAGCCTCATCGATG
GTGATAGTGTGCATCTTTGA

Upstream 100 bases:

>100_bases
TATTTAGGCCATCTTTCTTATGTATTGCAACCACCTCGCAGGTGGCTTGAACTATACCTGTAAACATTTTCTGCTCGCTT
AATCGTTAAAGGCTGTATTT

Downstream 100 bases:

>100_bases
TAGCATTAACGGCGAGCATCGATGCTCGCCGTTTTTTTATCGGTTTGATTTCGATGCCGTCTCTGGATTTAAACGGATAA
GAAACTGCGGTTTTATCAGC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNNSGYQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHYNGANN
QKAIQPLAFQGAYLAIIGGIGVMIFLASAPFVLGHMDLEPELYRLTIGYLDGILWGAPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPPMGGAGCGIATALVFWAMLIAMTIYMQLHKKFAALAPFSKFHRPDFETMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVTKVGLWL
ALSLALSTAVLTVIFRFQIAELYNGDPEVVALAGSLMLMAALYQLSDSVQVVAAGALRGYKDTRSAFYITLFSYWGIGMS
LGYTLAYTDHIVPAMGAHGFWAGLIAGLTSAALLFFIRLRYIQKHGVHLSLIDGDSVHL

Sequences:

>Translated_459_residues
MNNSGYQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHYNGANN
QKAIQPLAFQGAYLAIIGGIGVMIFLASAPFVLGHMDLEPELYRLTIGYLDGILWGAPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPPMGGAGCGIATALVFWAMLIAMTIYMQLHKKFAALAPFSKFHRPDFETMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVTKVGLWL
ALSLALSTAVLTVIFRFQIAELYNGDPEVVALAGSLMLMAALYQLSDSVQVVAAGALRGYKDTRSAFYITLFSYWGIGMS
LGYTLAYTDHIVPAMGAHGFWAGLIAGLTSAALLFFIRLRYIQKHGVHLSLIDGDSVHL
>Mature_459_residues
MNNSGYQAKRLIQLALPVLIAQVTQTMMGFIDTVMAGRVSAVDMAAVAVGTSLWLPAILFVQGLLMAFTPLFAHYNGANN
QKAIQPLAFQGAYLAIIGGIGVMIFLASAPFVLGHMDLEPELYRLTIGYLDGILWGAPAFVLYQVLRGCSEGISYTLPTM
VIGFVGLAVNIPANYIFIYGHFGVPPMGGAGCGIATALVFWAMLIAMTIYMQLHKKFAALAPFSKFHRPDFETMKKMTKL
GMPIAMALFFEVSLFAIIALLLAPLGATVVASHQIALNFSAIVFMLPLSIGIAVSIRIGYYLGRDRADISAVVTKVGLWL
ALSLALSTAVLTVIFRFQIAELYNGDPEVVALAGSLMLMAALYQLSDSVQVVAAGALRGYKDTRSAFYITLFSYWGIGMS
LGYTLAYTDHIVPAMGAHGFWAGLIAGLTSAALLFFIRLRYIQKHGVHLSLIDGDSVHL

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=454, Percent_Identity=23.1277533039648, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI22907060, Length=460, Percent_Identity=21.9565217391304, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38261960, Length=490, Percent_Identity=22.2448979591837, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI48994890, Length=439, Percent_Identity=51.25284738041, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI226510954, Length=458, Percent_Identity=23.3624454148472, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6321821, Length=399, Percent_Identity=22.8070175438596, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320545, Length=464, Percent_Identity=20.9051724137931, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49447; Mature: 49447

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]