Click here to switch to the map view.

The map label for this gene is emrD [H]

Identifier: 126174306

GI number: 126174306

Start: 2419077

End: 2420309

Strand: Reverse

Name: emrD [H]

Synonym: Sbal_2085

Alternate gene names: 126174306

Gene position: 2420309-2419077 (Counterclockwise)

Preceding gene: 126174307

Following gene: 126174304

Centisome position: 47.2

GC content: 49.55

Gene sequence:

>1233_bases
ATGTCAGGCACGCCACACTCTCCTATTCATCACTATGTCGCTCCAGCGGCGAGTCAGTCTAGTTTACTGTTAATGATTGT
CTTTATGGTCGCCTGTGGCCAAATGGCGCAGACGATTTTTGTGCCTGCCCTTCCGCAAATTGCACTCGGGCTCCATGTGG
ACGCCAGTCAGCTACAAGCAGTGATGGCCTGTTACTTACTCTCCTACGGGATCTTTCAATTTGTGTATGGCCCAGTGTCA
GATCGCGTTGGTCGTAAATTGCCACTCGTCGTGGGTATCGCTATTTTCATCATAGGAGCAGTGTTTGCGGCTCAGGCCAC
AAGCTTTAACCAACTGATTATGGCGAGCCTTATCCAAGGATTAGGCACAGCTAGCGCGGGCGCATTATGCCGGAGCATTC
CCCGCGATCATTACTTTGGCGACCGACTGGTTAAATTTAATAGTTATGTTTCTATGGCCGTAGTGTTTTTACCTTTACTG
GCACCATTTATCGGTAGCGCCAGTGCGGCCTACTTCGGCTGGCAAGCCGTGTACTGGGTGTTAGCAGGATTCGGTGCCGT
GGTCTGGCTGTTATTGATATTCGCCTTTAAAGAGTCATTACCTAAGGCGCAGCGGGAACATCAAGCCGTTATCGCATCCT
ATCGGCATGTCCTGCGCCATAAAGCGTTTCGAGCTTACTTATTTTCTTTGATCGCGACCTTTGCGGGTATTGCGGCGTTT
GAAGCTATTGCCGGCGTTCTCTATGGCCAATATCTTGGACTGACGCCCTTCATGGTCAGTTGTTATTTTGTGGCGCCGAT
TCCAGGCTATTTATTGGGCGCGCTGATCGCAGGTCGACAAAATAAGCCGCAAAGAGTTATCTATCAAGGTGTTGCGCTGC
TTGGCGCTGGCGCCTTATTCATGTTGATCCCGGGACTTTTCAATTTAGTCGTCGGTTGGAGTCTACTGGTTGGATCGATT
TTATTCTTCAGTGGCGCCGGCATGCTGTTCCCTACCCTGACCTCGGCGGCATTAGAGCCATTTCCACGTCAAGCAGGAAT
TGCGGGGGCATTACTGGGAGGATTACAAAATTTTGGTGCCGGACTTGCCGCATTGACTATGTCTTTAGTGCCTATGGGCG
GCCAGTTGAGCATAGGTTTATTAAGCGCTGTCATGGTGGTGTTAGTGATTTTGTCTTTGTATTACGCCAGAGATCAAAAT
GACACTCACCACAATTTGATGCCACTGATTTAA

Upstream 100 bases:

>100_bases
AAAAAATATTTCATCAAATCAATATGATTGACACTTGAAGTTACGCTTTTAGGGAGTAAGATGCGCGCAAATACCTTAAG
TGACCTTTTTCGGAAATATC

Downstream 100 bases:

>100_bases
TACCCTAAATTTAAGATCCTAGATTTAAAACTATAGATTTAAAACCATAAATTTAATGCCGAAAAATTGAAGCGATAGCG
TTCTTAGCTTAAGCAACATC

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSGTPHSPIHHYVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGIFQFVYGPVS
DRVGRKLPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLL
APFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHKAFRAYLFSLIATFAGIAAF
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVGWSLLVGSI
LFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAVMVVLVILSLYYARDQN
DTHHNLMPLI

Sequences:

>Translated_410_residues
MSGTPHSPIHHYVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGIFQFVYGPVS
DRVGRKLPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLL
APFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHKAFRAYLFSLIATFAGIAAF
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVGWSLLVGSI
LFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAVMVVLVILSLYYARDQN
DTHHNLMPLI
>Mature_409_residues
SGTPHSPIHHYVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGIFQFVYGPVSD
RVGRKLPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLLA
PFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHKAFRAYLFSLIATFAGIAAFE
AIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVGWSLLVGSIL
FFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAVMVVLVILSLYYARDQND
THHNLMPLI

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=345, Percent_Identity=43.768115942029, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788509, Length=329, Percent_Identity=27.355623100304, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994889, Length=369, Percent_Identity=25.4742547425474, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790146, Length=381, Percent_Identity=27.8215223097113, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790794, Length=299, Percent_Identity=22.4080267558528, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43728; Mature: 43596

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGTPHSPIHHYVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQA
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
VMACYLLSYGIFQFVYGPVSDRVGRKLPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQG
HHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLLAPFIGSASAAYFGWQAVYWV
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHKAFRAYLFSLIATFAGIAAF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALF
HHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MLIPGLFNLVVGWSLLVGSILFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHH
GLAALTMSLVPMGGQLSIGLLSAVMVVLVILSLYYARDQNDTHHNLMPLI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]