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The map label for this gene is hemB [H]

Identifier: 126174084

GI number: 126174084

Start: 2158129

End: 2159112

Strand: Reverse

Name: hemB [H]

Synonym: Sbal_1858

Alternate gene names: 126174084

Gene position: 2159112-2158129 (Counterclockwise)

Preceding gene: 126174092

Following gene: 126174082

Centisome position: 42.11

GC content: 52.13

Gene sequence:

>984_bases
ATGTTAAACACGCCTCCATTGCGTCGCCTAAGACGCTTACGTAGTTCAGAAGCGATGCGCGATCTGCTGCGCGAAACCCA
TATTTCGTTAAGCGATCTCATCTATCCCCTGTTCATAGAAGAACACATCACCCACGCAGTGCCTATCTCCACGCTACCAG
GTATCAGCCGTTTACCAGAGAGTGCACTGGCGGATGAAGTGCGCGAACTTTACGCCCTCGGGATCCGTTATGTAATGCCC
TTTGGGATTTCGCACACTAAGGATGATAAAGGCAGCGACACGTGGGATGACAATGGCTTGCTCGCCCGCATGGTGCGTAC
CATTAAAGCCGCCGTGCCTGAGATGATGGTGATCCCCGATATTTGTTTTTGTGAATATACAGATCACGGCCATTGCGGCG
TGGTGCACAATAATGAAGTCTGCAACGATCTCACCGTCGAAAATTTAGTTAAGCAATCGGTGACTGCCGCCAAAGCGGGC
GCCGACATGTTAGCGCCATCGGCCATGATGGACGGTCAAGTGAAAGCCATTCGCCAAGGGCTTGATGCCGCAGGTTTTGA
GCATGTGGCGATTCTGGCCCACTCGGCAAAATTCGCCTCTTCCTTCTACGGTCCTTTCAGAGCGGCAGTCGATTGTGAAC
TGAGCGGTAACCGCAAGGGATATCAGCTTGATTATGCCAACGGTCGCCAAGCCCTGTTGGAAGCCTCATTAGATGAAGCC
GAAGGCGCTGATATTCTGATGGTTAAACCCGGTACGCCCTACTTAGATGTGTTAAGCCGTTTAAGACAAGAAACCCACTT
GCCACTCGCCGCCTATCAAGTCGGCGGCGAATATGCAGGCATCAAGTTTGCCGCCCTTGCAGGTGCGTTGGATGAGCGTG
CTGTGGTGACTGAAACGTTTGTCGGCTTAAAGCGGGCCGGAGCGGATTTGATTGTCAGCTATTACACCAAACAATATGCC
CAGTGGTTAGCCGAGAGTAGATAG

Upstream 100 bases:

>100_bases
ACTTTGCTTGTCAGAGCAGACAAGTCTGGATTGAATTCATTCCTTGCCATTCCTTTACTATTGTACGATTGCACTAACCC
GCCTTCGATAAGGATATTTC

Downstream 100 bases:

>100_bases
TTACCTCGGTAACTACCATTTTGGATGCTAAAAAAATCTATAAAAAACGCCACCTTTATCGGTGGCGTTTTAGTATTTAG
CATCCTGCGCTGAATTGCTT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETHISLSDLIYPLFIEEHITHAVPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRTIKAAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEASLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFVGLKRAGADLIVSYYTKQYA
QWLAESR

Sequences:

>Translated_327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETHISLSDLIYPLFIEEHITHAVPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRTIKAAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEASLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFVGLKRAGADLIVSYYTKQYA
QWLAESR
>Mature_327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETHISLSDLIYPLFIEEHITHAVPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRTIKAAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEASLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFVGLKRAGADLIVSYYTKQYA
QWLAESR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=40.5228758169935, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=56.9182389937107, Blast_Score=360, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=38.5350318471338, Blast_Score=208, Evalue=9e-55,
Organism=Drosophila melanogaster, GI21358291, Length=293, Percent_Identity=39.9317406143345, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35912; Mature: 35912

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTPPLRRLRRLRSSEAMRDLLRETHISLSDLIYPLFIEEHITHAVPISTLPGISRLPE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
SALADEVRELYALGIRYVMPFGISHTKDDKGSDTWDDNGLLARMVRTIKAAVPEMMVIPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
ICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAGADMLAPSAMMDGQVKAIRQG
EEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHC
LDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEASLDEA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHC
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETF
CCCCEEEECCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
VGLKRAGADLIVSYYTKQYAQWLAESR
HHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNTPPLRRLRRLRSSEAMRDLLRETHISLSDLIYPLFIEEHITHAVPISTLPGISRLPE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
SALADEVRELYALGIRYVMPFGISHTKDDKGSDTWDDNGLLARMVRTIKAAVPEMMVIPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
ICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAGADMLAPSAMMDGQVKAIRQG
EEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHC
LDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEASLDEA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHC
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETF
CCCCEEEECCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
VGLKRAGADLIVSYYTKQYAQWLAESR
HHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656410; 2464127; 9278503; 8439296; 10194344; 11444968; 11909869 [H]