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The map label for this gene is rimK [H]

Identifier: 126174074

GI number: 126174074

Start: 2148436

End: 2149422

Strand: Reverse

Name: rimK [H]

Synonym: Sbal_1848

Alternate gene names: 126174074

Gene position: 2149422-2148436 (Counterclockwise)

Preceding gene: 126174075

Following gene: 126174073

Centisome position: 41.92

GC content: 44.28

Gene sequence:

>987_bases
ATGCGTGGTTGGATTTTATATAAAGAAACCGCGACTCAGCTAAAACCTGAATGGTATGAAATTGAGCGTCTACTGGCGGC
AGCAAAAGCCGACAACATACAGTTGGAAGTTTACGCACCTGATGAATTTGATCTGACTGTCACCAGAGAAGATAACAAGA
GTATTTTATTGAATGGGCAAGCCGTCGAGCTGCCCGATTTTATTATTCCGCGAATGGGATCAGGCACAACTTACTTTGCC
TTAGCCATTATTCGCCACTTAGAAAGATTAGGGGTGTATTGCCTCAATCCTTCTAAAGCCATTGAAATCGTTAAAGACAA
ATTATTCTCCCAGCAATTGTTGGCCGAGAAGAATTTGCCTACGCCTAAAACTATGTTAGTTAAGTTCCCCGTTGATATCG
CCCTCGTAGAGCGCCATCTCGGCTTTCCTGTGGTGATTAAAACGCTTTCTGGCTCACAGGGCAGCGGTGTGTTTTTATCC
CATAAGGCACGTGAATTTGACGATCTAATGCAGTTAATTGAAGCGACCAACAAAAACGCTAACATTATTCTGCAAGAGTT
TATCGCTAACAGCCATGGCCGCGATCTTCGGGTATTTACCATCGGTGGTCGTGTTGCAGGTTGCTACGAAAGGCGTGGTC
AAGAAGACAGCTTTAAAGCCAATGTCAGTGCCGGCGGCTCAGCGCGTCCATTTCAGATCACTCCTGAAATTGAATGGCTT
GCCACGCAAACAGCCAATATTCTCGATCTCGATGTTGCGGGTATCGATTTATTGTTTGATAACGGCCATTACAAAATTTG
CGAAGCAAACTCATCCCCAGGATTTGAAGGCTTAGAATCCTGTTTGAATGTGGATATTGCCGCGCAAATTTTACACTTTA
TTCGCATCCGTTTAGGCATGTTTAATAAAGATGAGCCAAGTAGCCCCAAAGTGGTCATTACTGACAATGTAGAAACGTCT
GTGACGCCCGTTATCGAGCCTAAATAG

Upstream 100 bases:

>100_bases
AGATAGAAGTTGAGGCCGAGGCTGTATTTGATATTGAAGATGATGTTCAGGATGAACCTGAACCCGAGGATAAAGAATGA
AGATTAAGGTTTGCTACTAA

Downstream 100 bases:

>100_bases
CGGATGAGGGACAACTTAATATCTAGCGGTATCACGCCGCTAGATATTCTTAAATTGATTACTATCCCCCATTTATCATG
TTTCACATTCATTGGCATAT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKVVITDNVETS
VTPVIEPK

Sequences:

>Translated_328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKVVITDNVETS
VTPVIEPK
>Mature_328_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDEPSSPKVVITDNVETS
VTPVIEPK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=297, Percent_Identity=27.6094276094276, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI134254462, Length=297, Percent_Identity=29.6296296296296, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787076, Length=277, Percent_Identity=34.2960288808664, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 36531; Mature: 36531

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCHHEEHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
EEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEPSSPKVVITDNVETSVTPVIEPK
CCCCCCCCCEEEEECCCCCCCCCEECCC
>Mature Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIQLEVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIALVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCHHEEHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
EEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDEPSSPKVVITDNVETSVTPVIEPK
CCCCCCCCCEEEEECCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA