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The map label for this gene is miaE [H]

Identifier: 126173832

GI number: 126173832

Start: 1867103

End: 1867873

Strand: Direct

Name: miaE [H]

Synonym: Sbal_1598

Alternate gene names: 126173832

Gene position: 1867103-1867873 (Clockwise)

Preceding gene: 126173830

Following gene: 126173835

Centisome position: 36.41

GC content: 45.53

Gene sequence:

>771_bases
ATGCAGCAACTTCTTGCCCCCATCCACGCGTTTCTTCGCTGTGAAACACCGCAAACTTGGATTGATGTCGCGATAAAATC
AGATTCCTTGGATGAGTTGCTTATCGATCATTGTAACTGTGAGTTAAAAGCGGCCCAGACGGCGATATTTTTGCTACGAA
AATATGCGTTAGATAAAGACAGTGGTCGAGCTCTGTTGGCGTGGGCTAAACCCTATGAAGAGTTTATCTATTCGCAAGAT
CATGATGTGGTGAGCTTTCTGGCGCGCGATGTGAAAAAGAATGAGCTGGTGGCCGAACTTATGCCTAAGCCAAACTTTAA
GTTTGCCGCTAATTTGGTTTCACCTTTAATCCGCTTGATTAAAGAAGAGTTTCATCATTTTGAGCAAGTATTGGAATTGA
TGCACAAACGCAGCATTGAGTATCGTAATATTCGCGCAGGCCGTTATGCCAAAGGCATGATGAGCTATGTGACGACCCAT
GAGAAAGATATCCTGCGGGATAAGCTGATTGTGGGGGCATATATTGAGGCGCGTTCGTGTGAGCGTTTTGCTAAGTTGGC
GCCCTATTTAGACGGTGAGCTGAGTAAGTTTTATGTGTCACTGCTGCGCTCTGAAGCCCGCCACTATCAAGATTATCTTT
CATTAGCACAGTTGGTTTCACCTGAGGATATTAGCGAGCGCGTAAAGTACTTTGGTGAGTTAGAGGCGGAGTTGATTCTG
GCGCAGGATGAAGAGTTTTGTTTCCACAGTGGATCGCCGCTAGCAAATTGA

Upstream 100 bases:

>100_bases
AAAAAGTTCGGCGCGTCAAGTTTACTGAAGCCTAATGCTATAGCTTATGAGTAAATATGCGTAAAATAGCGCGATATTCA
GAATGATATTGAGTGAGTCC

Downstream 100 bases:

>100_bases
TTTGAAGCACCTTCTTTTTAAAAATTGATGAATTAAGTCGATATAGCTAAAACGATTAACGGTTCGCTCGAACCGTTAAT
ATGAAGCCTCTTTAAGCTGA

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLDELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLSLAQLVSPEDISERVKYFGELEAELIL
AQDEEFCFHSGSPLAN

Sequences:

>Translated_256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLDELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLSLAQLVSPEDISERVKYFGELEAELIL
AQDEEFCFHSGSPLAN
>Mature_256_residues
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLDELLIDHCNCELKAAQTAIFLLRKYALDKDSGRALLAWAKPYEEFIYSQD
HDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLIKEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTH
EKDILRDKLIVGAYIEARSCERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLSLAQLVSPEDISERVKYFGELEAELIL
AQDEEFCFHSGSPLAN

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29623; Mature: 29623

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLDELLIDHCNCELKAAQTAIFLLRKYALDKD
CHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
SGRALLAWAKPYEEFIYSQDHDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLI
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTHEKDILRDKLIVGAYIEARSC
HHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLSLAQLVSPEDISERVKYFGELEAELIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEE
AQDEEFCFHSGSPLAN
ECCCHHHCCCCCCCCC
>Mature Secondary Structure
MQQLLAPIHAFLRCETPQTWIDVAIKSDSLDELLIDHCNCELKAAQTAIFLLRKYALDKD
CHHHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
SGRALLAWAKPYEEFIYSQDHDVVSFLARDVKKNELVAELMPKPNFKFAANLVSPLIRLI
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEEFHHFEQVLELMHKRSIEYRNIRAGRYAKGMMSYVTTHEKDILRDKLIVGAYIEARSC
HHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERFAKLAPYLDGELSKFYVSLLRSEARHYQDYLSLAQLVSPEDISERVKYFGELEAELIL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEE
AQDEEFCFHSGSPLAN
ECCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]