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The map label for this gene is ybjS [C]

Identifier: 126173787

GI number: 126173787

Start: 1806734

End: 1807942

Strand: Direct

Name: ybjS [C]

Synonym: Sbal_1553

Alternate gene names: 126173787

Gene position: 1806734-1807942 (Clockwise)

Preceding gene: 126173786

Following gene: 126173788

Centisome position: 35.24

GC content: 48.97

Gene sequence:

>1209_bases
ATGACGATAGACCCAATCAAGAGCACGACGCTTGCGCAATTTGATGCGGCTGAACAAACTGCACTCGCGCAACTCGCCCT
TAAGGTGCAACACGCCTTTGTGACGGGAGCGGGGGGATTTTTAGGTAAAGCGATTTGTTTGAGATTGTTAGCCGCGGGCA
TTAAGGTCACAGGTTTTGCCCGTGGGCATTATCCCGAACTTGAAGCGCTGGGCGTAGTCATGTTGCAAGGGGATTTAGTC
AATAAAGACCAGTTACAACAGGCAATGCAAGGCTGCGATATCGTGTTCCATGTGGCGTCTAAAGCCGGTGTTTGGGGCGA
TAGAGACAGTTATTTTTGCCCGAATGTTAAGGGCGCCGCAAATGTGATTGCTGCTTGTAAAACATTAAAAATAAATAAGT
TAGTCTACACCAGTACCCCGAGTGTGACCTTTGCTGGGCAGGATGAATCCGGCATAGATGAATCCACTCCCTATGCCACA
TCCTTCCTCAATTATTATGCCCATTCCAAAGCTATTGCCGAAAAGATGATGCTTGATGCCAATCAAGTGGGTGACGTATC
GGTTGAAAATACCGCTGTAACTCAAGTAACTCAAGTGACTCAAGCCACGACAGCCACTATTTCAGACAATAATCTAGCCA
CTACTCAAGTCGCTATCCCGAACACATCTCAAGTCACTGCACCCTATGCGTTAAAAACCGTCGCGCTCAGGCCGCATTTG
ATCTGGGGTCCTGGCGATCCCCATCTTGTACCTCGAGTACTGGCGCGCTGCCGTTTAGATAAGCTTAAACTGGTTGGCCA
TGAAGATAAGCTGGTCGATACCATCTACATAGACAACGCCGCCTATGCCCATGTGCTGGCCGCGCTTGAACTGTGCCAAG
CAAAGCCTAAGTGCCAGGGGAAAGCTTATTTTTTGAGTAACGATGAACCCATCACTATGGCCAAAATGCTCAATCTGATT
CTGGCCTGCGATGCTTTGCCGCCAGTTACTAAGCGTGTGCCGCAATCTGTGGCCTATGTGGCAGGCGCCGTGTTGGAAAC
AGTGTATTTTCTACTAAAGAAGCAAGAAGAGCCCATGATGACGCGCTTCGTGGCACGGCAGTTATCCTGCAGCCATTATT
TTGATATCAGCGCCGCCAAGCGAGATTTAGGCTATCGTGCACTTGTCTCTATCAATGAAGGGATGGCAAGACTTAAAGCG
TCACTCTAG

Upstream 100 bases:

>100_bases
TTCATTTGAACAAACTCGCTTATTTTTAAGCCCTTGTGCTTATTTTAAACTTCTTACACCGACTTTAAGTTCATGTAAAT
GTGCTGAAATGGATACCTAA

Downstream 100 bases:

>100_bases
TGTTATTAGGCGTGAGTTTCGGAGTGTGCTTGATATTCAATATGCCTGCTCTTATTTGCACTCTTCTTGGCACTATTATT
GTGGGCATAGTTTAGCTGGG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTIDPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLV
NKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKTLKINKLVYTSTPSVTFAGQDESGIDESTPYAT
SFLNYYAHSKAIAEKMMLDANQVGDVSVENTAVTQVTQVTQATTATISDNNLATTQVAIPNTSQVTAPYALKTVALRPHL
IWGPGDPHLVPRVLARCRLDKLKLVGHEDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLI
LACDALPPVTKRVPQSVAYVAGAVLETVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALVSINEGMARLKA
SL

Sequences:

>Translated_402_residues
MTIDPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLV
NKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKTLKINKLVYTSTPSVTFAGQDESGIDESTPYAT
SFLNYYAHSKAIAEKMMLDANQVGDVSVENTAVTQVTQVTQATTATISDNNLATTQVAIPNTSQVTAPYALKTVALRPHL
IWGPGDPHLVPRVLARCRLDKLKLVGHEDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLI
LACDALPPVTKRVPQSVAYVAGAVLETVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALVSINEGMARLKA
SL
>Mature_401_residues
TIDPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLVN
KDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKTLKINKLVYTSTPSVTFAGQDESGIDESTPYATS
FLNYYAHSKAIAEKMMLDANQVGDVSVENTAVTQVTQVTQATTATISDNNLATTQVAIPNTSQVTAPYALKTVALRPHLI
WGPGDPHLVPRVLARCRLDKLKLVGHEDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLIL
ACDALPPVTKRVPQSVAYVAGAVLETVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALVSINEGMARLKAS
L

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=374, Percent_Identity=29.6791443850267, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI8393516, Length=374, Percent_Identity=29.6791443850267, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI116268111, Length=366, Percent_Identity=28.4153005464481, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI239745448, Length=369, Percent_Identity=27.9132791327913, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310132178, Length=369, Percent_Identity=27.9132791327913, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310113012, Length=369, Percent_Identity=27.9132791327913, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI260763931, Length=386, Percent_Identity=27.4611398963731, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4504509, Length=386, Percent_Identity=27.4611398963731, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI87081792, Length=376, Percent_Identity=23.6702127659574, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321437, Length=392, Percent_Identity=29.8469387755102, Blast_Score=133, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 43424; Mature: 43292

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFA
CCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEE
RGHYPELEALGVVMLQGDLVNKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAA
CCCCCCHHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCHHH
NVIAACKTLKINKLVYTSTPSVTFAGQDESGIDESTPYATSFLNYYAHSKAIAEKMMLDA
HHHHHHHHEEECEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NQVGDVSVENTAVTQVTQVTQATTATISDNNLATTQVAIPNTSQVTAPYALKTVALRPHL
CCCCCCEECCHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCCCHHHEEEEECCEE
IWGPGDPHLVPRVLARCRLDKLKLVGHEDKLVDTIYIDNAAYAHVLAALELCQAKPKCQG
EECCCCCCHHHHHHHHHCHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCC
KAYFLSNDEPITMAKMLNLILACDALPPVTKRVPQSVAYVAGAVLETVYFLLKKQEEPMM
EEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TRFVARQLSCSHYFDISAAKRDLGYRALVSINEGMARLKASL
HHHHHHHHCCCCEEECHHHHHCCCCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
TIDPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFA
CCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEE
RGHYPELEALGVVMLQGDLVNKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAA
CCCCCCHHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCHHH
NVIAACKTLKINKLVYTSTPSVTFAGQDESGIDESTPYATSFLNYYAHSKAIAEKMMLDA
HHHHHHHHEEECEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NQVGDVSVENTAVTQVTQVTQATTATISDNNLATTQVAIPNTSQVTAPYALKTVALRPHL
CCCCCCEECCHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCCCHHHEEEEECCEE
IWGPGDPHLVPRVLARCRLDKLKLVGHEDKLVDTIYIDNAAYAHVLAALELCQAKPKCQG
EECCCCCCHHHHHHHHHCHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCC
KAYFLSNDEPITMAKMLNLILACDALPPVTKRVPQSVAYVAGAVLETVYFLLKKQEEPMM
EEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TRFVARQLSCSHYFDISAAKRDLGYRALVSINEGMARLKASL
HHHHHHHHCCCCEEECHHHHHCCCCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]