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The map label for this gene is maiA [H]

Identifier: 126173726

GI number: 126173726

Start: 1730307

End: 1730957

Strand: Direct

Name: maiA [H]

Synonym: Sbal_1488

Alternate gene names: 126173726

Gene position: 1730307-1730957 (Clockwise)

Preceding gene: 126173725

Following gene: 126173727

Centisome position: 33.75

GC content: 50.08

Gene sequence:

>651_bases
ATGAAACTTTATGGTTATTGGCGCTCAAGCGCGGCGTACCGAGTTCGCATCGCCCTCAATCTTAAGGGTATTTCAGCCGA
GCAGTTGTCAGTGCATCTTGTACGTGATGGCGGCGAGCAACATAAAGCGGCCTACAGTGCGCTCAATCCGCTGGAGTTAG
TGCCAACCTTAATGATGGACGATGAGTTAGATGCGGATGCTTTGTCGCAATCCTTAGCCATTATCGAATATCTTGATGAG
ATACACCCACAGTCACCATTATTGCCTGCATCGGCTCTTGAGCGTGCCCATGTGCGCGCCATGGCACTCACGGTAGCCTG
TGAAATTCACCCATTAAATAATTTGCGGGTATTGCAGTATTTAACCCAAACCTTAGGGGTCGATGAAGCGGCTAAAAATA
CTTGGTATCACCACTGGGTCGCGAGTGGCTTTGCAGCCTTAGAAACTTTGTTGGTAAGGCACAGCGGTCGTTACTGCTTT
GGGGATACAGTGACATTAGCCGACCTGTGTTTAGTGCCGCAGGTGTATAACGCTCAGCGTTTTAATGTGGATTTAACGCC
TTATCCCAATATCATGCGCGTGTGGACTGAGTGTAATCAGCTAGAAGCCTTTGCCGATGCGGCCCCGGAGCGCCAAGCGG
ATGCTGTGTGA

Upstream 100 bases:

>100_bases
ATACAAAGCCTAAGGCTTTCCCTTAAAGGGCTACATCGACTCAAACTAACGGGGCTTAATTGCCCCGTTTTTATACTGAC
TTTTATAAGGATATGAAAAT

Downstream 100 bases:

>100_bases
TGCTTTGTAACGAATAGTGCGGATATAGTGTTTAGCAATTGAAAGAATAGATGATGAGGCTTGTGTTGCGAGTCTTATCT
CAGCGATGGAGTTTAAATGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLMMDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV

Sequences:

>Translated_216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLMMDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
>Mature_216_residues
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLMMDDELDADALSQSLAIIEYLDE
IHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQYLTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCF
GDTVTLADLCLVPQVYNAQRFNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=213, Percent_Identity=48.3568075117371, Blast_Score=194, Evalue=4e-50,
Organism=Homo sapiens, GI194440732, Length=213, Percent_Identity=38.9671361502347, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI22202622, Length=165, Percent_Identity=46.6666666666667, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17551302, Length=212, Percent_Identity=44.3396226415094, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17510461, Length=213, Percent_Identity=40.3755868544601, Blast_Score=156, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17556142, Length=214, Percent_Identity=40.1869158878505, Blast_Score=146, Evalue=7e-36,
Organism=Drosophila melanogaster, GI21355859, Length=213, Percent_Identity=45.5399061032864, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24645375, Length=213, Percent_Identity=45.5399061032864, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45553325, Length=213, Percent_Identity=45.5399061032864, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=38.9671361502347, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24135; Mature: 24135

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLMMD
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCC
DELDADALSQSLAIIEYLDEIHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCHHHHHH
LTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCFGDTVTLADLCLVPQVYNAQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCHHCCCEE
FNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLYGYWRSSAAYRVRIALNLKGISAEQLSVHLVRDGGEQHKAAYSALNPLELVPTLMMD
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCC
DELDADALSQSLAIIEYLDEIHPQSPLLPASALERAHVRAMALTVACEIHPLNNLRVLQY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCHHHHHH
LTQTLGVDEAAKNTWYHHWVASGFAALETLLVRHSGRYCFGDTVTLADLCLVPQVYNAQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCHHCCCEE
FNVDLTPYPNIMRVWTECNQLEAFADAAPERQADAV
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]