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The map label for this gene is mak [H]

Identifier: 126173713

GI number: 126173713

Start: 1715556

End: 1716473

Strand: Direct

Name: mak [H]

Synonym: Sbal_1475

Alternate gene names: 126173713

Gene position: 1715556-1716473 (Clockwise)

Preceding gene: 126173712

Following gene: 126173714

Centisome position: 33.46

GC content: 52.83

Gene sequence:

>918_bases
ATGATGCGCATGGGCGTCGATTTAGGCGGAACAAAAATAGAATTAGTCGCACTGAGTGATGAAGGCAATGAGCTATTTCG
CAAACGTGTGACGACGCCGCGTGACTATCAGGGCACGCTAGCTGCGGTTGTCAATCTGGTCAAGGAAGCGGAAGCGACAC
TCGGCGAGCAAGGCACAGTCGGTGTGGGAATTCCAGGCGTGGTATCGCCCTATTCAGGTTTAGTGAAAAACGCCAATTCA
ACATGGATCAATGGCCATCCCCTCGATGTGGATTTAGGGGAATTGTTGCAGCGTGAAGTGCGGGTTGCCAATGATGCAAA
CTGCTTTGCGGTATCAGAGTCGGTCGATGGTGCCGCCGCTGGCGCATCTGTGGTGTTTGGTGTGATTCTCGGCACTGGCT
GTGGCGCTGGCGTGGCAATTAACGGCAAAGTGCACGGTGGGGGTAATGGTATTGGCGGTGAATGGGGTCATAATCCTCTG
CCTTGGATGACCAAGGAAGAGTTTAATACCACCCGTTGCTTCTGTGGCAATCCCGATTGTATCGAGACCTTTATCTCTGG
CACTGGCTTTGTGCGTGATTATAATGCGGCTTTGACGGCGGCGGGCACAGTGCGTGCTGCTGCAAAATCTGGTGCCGATA
TTATGTTGTTAATCGATGAGGGTGATGCGATTGCGGTCGCTGCATTTGATCGCTATATGGATAGGCTCGCTCGTTCACTC
GCCCATGTGATCAACATGCTTGATCCCGATGCCATAGTGCTGGGCGGCGGTATGTCTAATGTTGCGGCAATTTACCCAAG
ATTACCGGCATTACTGGCCCATTATGTGGTGGGGCGTGAGTGCCATACGCCTGTGGTGCAGAATCTTTATGGTTGTTCAT
CGGGTGTGCGCGGCGCCGCTTGGTTGTGGGAAAAGTAA

Upstream 100 bases:

>100_bases
CCTTACTTTATTGCCACAGCCCCAGGCTGGGTGAAATAACGACCGCCTAGCATCGATATTCGTTGATGAATGGGCGGGCT
AAACACTGGAGTAGTAATTC

Downstream 100 bases:

>100_bases
TCTTTGCTTGGATATCTGTACTCATCTAGTGAGCTTATTAGGCTTTGAAATCAATGCTTAATGGCCAATACGTTTTATAA
ACATTAGCCCAGCGAAACGC

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MMRMGVDLGGTKIELVALSDEGNELFRKRVTTPRDYQGTLAAVVNLVKEAEATLGEQGTVGVGIPGVVSPYSGLVKNANS
TWINGHPLDVDLGELLQREVRVANDANCFAVSESVDGAAAGASVVFGVILGTGCGAGVAINGKVHGGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNAALTAAGTVRAAAKSGADIMLLIDEGDAIAVAAFDRYMDRLARSL
AHVINMLDPDAIVLGGGMSNVAAIYPRLPALLAHYVVGRECHTPVVQNLYGCSSGVRGAAWLWEK

Sequences:

>Translated_305_residues
MMRMGVDLGGTKIELVALSDEGNELFRKRVTTPRDYQGTLAAVVNLVKEAEATLGEQGTVGVGIPGVVSPYSGLVKNANS
TWINGHPLDVDLGELLQREVRVANDANCFAVSESVDGAAAGASVVFGVILGTGCGAGVAINGKVHGGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNAALTAAGTVRAAAKSGADIMLLIDEGDAIAVAAFDRYMDRLARSL
AHVINMLDPDAIVLGGGMSNVAAIYPRLPALLAHYVVGRECHTPVVQNLYGCSSGVRGAAWLWEK
>Mature_305_residues
MMRMGVDLGGTKIELVALSDEGNELFRKRVTTPRDYQGTLAAVVNLVKEAEATLGEQGTVGVGIPGVVSPYSGLVKNANS
TWINGHPLDVDLGELLQREVRVANDANCFAVSESVDGAAAGASVVFGVILGTGCGAGVAINGKVHGGGNGIGGEWGHNPL
PWMTKEEFNTTRCFCGNPDCIETFISGTGFVRDYNAALTAAGTVRAAAKSGADIMLLIDEGDAIAVAAFDRYMDRLARSL
AHVINMLDPDAIVLGGGMSNVAAIYPRLPALLAHYVVGRECHTPVVQNLYGCSSGVRGAAWLWEK

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=308, Percent_Identity=58.4415584415584, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1787363, Length=307, Percent_Identity=40.3908794788274, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790522, Length=307, Percent_Identity=28.0130293159609, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786891, Length=283, Percent_Identity=24.3816254416961, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31892; Mature: 31892

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRMGVDLGGTKIELVALSDEGNELFRKRVTTPRDYQGTLAAVVNLVKEAEATLGEQGTV
CEEECCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
GVGIPGVVSPYSGLVKNANSTWINGHPLDVDLGELLQREVRVANDANCFAVSESVDGAAA
ECCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
GASVVFGVILGTGCGAGVAINGKVHGGGNGIGGEWGHNPLPWMTKEEFNTTRCFCGNPDC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYNAALTAAGTVRAAAKSGADIMLLIDEGDAIAVAAFDRYMDRLARSL
HHHHHCCCCCEEECCHHHHHCHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHH
AHVINMLDPDAIVLGGGMSNVAAIYPRLPALLAHYVVGRECHTPVVQNLYGCSSGVRGAA
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCC
WLWEK
CCCCC
>Mature Secondary Structure
MMRMGVDLGGTKIELVALSDEGNELFRKRVTTPRDYQGTLAAVVNLVKEAEATLGEQGTV
CEEECCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
GVGIPGVVSPYSGLVKNANSTWINGHPLDVDLGELLQREVRVANDANCFAVSESVDGAAA
ECCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
GASVVFGVILGTGCGAGVAINGKVHGGGNGIGGEWGHNPLPWMTKEEFNTTRCFCGNPDC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
IETFISGTGFVRDYNAALTAAGTVRAAAKSGADIMLLIDEGDAIAVAAFDRYMDRLARSL
HHHHHCCCCCEEECCHHHHHCHHHHHHHCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHH
AHVINMLDPDAIVLGGGMSNVAAIYPRLPALLAHYVVGRECHTPVVQNLYGCSSGVRGAA
HHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCC
WLWEK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]