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The map label for this gene is adaA [H]

Identifier: 126173646

GI number: 126173646

Start: 1621856

End: 1622830

Strand: Direct

Name: adaA [H]

Synonym: Sbal_1408

Alternate gene names: 126173646

Gene position: 1621856-1622830 (Clockwise)

Preceding gene: 126173641

Following gene: 126173649

Centisome position: 31.63

GC content: 46.05

Gene sequence:

>975_bases
ATGAAACCTCTGTGTGAAAAAGTGATCCCCGATGAAAACAGCTCTTGGCGTTACTTTCGTTATGAATGGGACCACATGGA
TCTCAACTGGCATTATCACCCCGAGTACGAAATCTGCTTAACCCTCAACAGTTATGGCATGCGTTATGTGGGCGACCATA
TTGCCCGCTATGGCGATTATGATTTAGTGATCATCGGCCCTGATATGCCCCACGGCTGGCAATCCGAGGCTAACCTTGAT
ATTTCACCTGACGAGGTTTCAAAGGATGACGGTTTGAAAGGCGGCACTTCAACTGCGGTTAATTTGAAAGAGCCAGCGTT
GAAGGGATCCGCTTCGAAAAGTCGAGTGCCGGAAGTCGTGTATGTGGCACAAATCCCCGCAGCCTGGCTACACCAAATTG
TGAACGATCAGCGCGAGTTAGCTTCCTTGCATTCGCTTTTGACCCATGCCAAACGGGGCGTGTTGTTTAGTGAGGAAACG
GCGCGCAAAGCCCATAGCGTGTTTATCGCGATGGAGATGGCTAATCCCTTTAAACGCTATATTTTGTTATTGGAATTATT
AGCGTTATTACAACAGGATAGTCAGCAAGAACTCTTGTCGAGCACCTTGTTCACCCACGGCAGTAAACTCGACTTTACCG
TGAAAAAGCTCGATAAAGTGATTGAGCATATTTATGACAATTACACTCAGGCCTTGTCGGCACAGGATTTGGCAAAGTTG
GCGCACATGAGCACCAATCATTTCCATCGCTTCTTTAAAAAACGCACCGAGAAAACCTTAACTGAGTTTATCAATCAACT
CAGGATCGCTAAGGCGTGCAAGTTATTACTCTCGAACCAGAGCCCGATTTCAACCATCAGCGATCAGTGTGGCTTCAATA
ACTTATCTAATTTCAATCGACGCTTTATTCAATTTAAAGGCGTCACGCCGAGTCGCTTTCGTCAGCGTTTTAGCAGTAAA
CAAAGGGCGATTTAA

Upstream 100 bases:

>100_bases
ATTTAACAGGATGCAATAACGGCTAGCTTGAGTGAATGGCGTTGATCTGCAAGGGCTGATTGAAGCTAAGGTAAAGGAAT
GAGTAACTGGAGTTTAATGG

Downstream 100 bases:

>100_bases
CCCTTACTTTCACGAATGCTTAGGCTGAAAATACTGCTCCACTAAGGTTTGGCAGTAGCTTATTGCCTGGGCCGTGATCT
CCGCTGAAATCGGCTCAAAT

Product: helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPLCEKVIPDENSSWRYFRYEWDHMDLNWHYHPEYEICLTLNSYGMRYVGDHIARYGDYDLVIIGPDMPHGWQSEANLD
ISPDEVSKDDGLKGGTSTAVNLKEPALKGSASKSRVPEVVYVAQIPAAWLHQIVNDQRELASLHSLLTHAKRGVLFSEET
ARKAHSVFIAMEMANPFKRYILLLELLALLQQDSQQELLSSTLFTHGSKLDFTVKKLDKVIEHIYDNYTQALSAQDLAKL
AHMSTNHFHRFFKKRTEKTLTEFINQLRIAKACKLLLSNQSPISTISDQCGFNNLSNFNRRFIQFKGVTPSRFRQRFSSK
QRAI

Sequences:

>Translated_324_residues
MKPLCEKVIPDENSSWRYFRYEWDHMDLNWHYHPEYEICLTLNSYGMRYVGDHIARYGDYDLVIIGPDMPHGWQSEANLD
ISPDEVSKDDGLKGGTSTAVNLKEPALKGSASKSRVPEVVYVAQIPAAWLHQIVNDQRELASLHSLLTHAKRGVLFSEET
ARKAHSVFIAMEMANPFKRYILLLELLALLQQDSQQELLSSTLFTHGSKLDFTVKKLDKVIEHIYDNYTQALSAQDLAKL
AHMSTNHFHRFFKKRTEKTLTEFINQLRIAKACKLLLSNQSPISTISDQCGFNNLSNFNRRFIQFKGVTPSRFRQRFSSK
QRAI
>Mature_324_residues
MKPLCEKVIPDENSSWRYFRYEWDHMDLNWHYHPEYEICLTLNSYGMRYVGDHIARYGDYDLVIIGPDMPHGWQSEANLD
ISPDEVSKDDGLKGGTSTAVNLKEPALKGSASKSRVPEVVYVAQIPAAWLHQIVNDQRELASLHSLLTHAKRGVLFSEET
ARKAHSVFIAMEMANPFKRYILLLELLALLQQDSQQELLSSTLFTHGSKLDFTVKKLDKVIEHIYDNYTQALSAQDLAKL
AHMSTNHFHRFFKKRTEKTLTEFINQLRIAKACKLLLSNQSPISTISDQCGFNNLSNFNRRFIQFKGVTPSRFRQRFSSK
QRAI

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 37325; Mature: 37325

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLCEKVIPDENSSWRYFRYEWDHMDLNWHYHPEYEICLTLNSYGMRYVGDHIARYGDY
CCCHHHHHCCCCCCCEEEEEEEECEEEECEEECCCEEEEEEECCCCHHHHHHHHHHCCCC
DLVIIGPDMPHGWQSEANLDISPDEVSKDDGLKGGTSTAVNLKEPALKGSASKSRVPEVV
EEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCEE
YVAQIPAAWLHQIVNDQRELASLHSLLTHAKRGVLFSEETARKAHSVFIAMEMANPFKRY
EEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHEEEEHHHCCHHHHH
ILLLELLALLQQDSQQELLSSTLFTHGSKLDFTVKKLDKVIEHIYDNYTQALSAQDLAKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHMSTNHFHRFFKKRTEKTLTEFINQLRIAKACKLLLSNQSPISTISDQCGFNNLSNFNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHH
RFIQFKGVTPSRFRQRFSSKQRAI
EEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPLCEKVIPDENSSWRYFRYEWDHMDLNWHYHPEYEICLTLNSYGMRYVGDHIARYGDY
CCCHHHHHCCCCCCCEEEEEEEECEEEECEEECCCEEEEEEECCCCHHHHHHHHHHCCCC
DLVIIGPDMPHGWQSEANLDISPDEVSKDDGLKGGTSTAVNLKEPALKGSASKSRVPEVV
EEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCEE
YVAQIPAAWLHQIVNDQRELASLHSLLTHAKRGVLFSEETARKAHSVFIAMEMANPFKRY
EEECCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHEEEEHHHCCHHHHH
ILLLELLALLQQDSQQELLSSTLFTHGSKLDFTVKKLDKVIEHIYDNYTQALSAQDLAKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHMSTNHFHRFFKKRTEKTLTEFINQLRIAKACKLLLSNQSPISTISDQCGFNNLSNFNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHH
RFIQFKGVTPSRFRQRFSSKQRAI
EEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]