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The map label for this gene is 126173482

Identifier: 126173482

GI number: 126173482

Start: 1429633

End: 1431303

Strand: Direct

Name: 126173482

Synonym: Sbal_1244

Alternate gene names: NA

Gene position: 1429633-1431303 (Clockwise)

Preceding gene: 126173481

Following gene: 126173484

Centisome position: 27.88

GC content: 48.95

Gene sequence:

>1671_bases
ATGGATAGACGTCAATTTCTAGCAGGCTCTGCCGCCCTAATGACGACTGCTGTTTGGGCGAGTTCAGTGGATAAATTACT
GGCACAGGATAACGCACAAGCGGTCGCCCGTGAGCCATGGGCCATAGGTTTTAGCGGGGCGGAAGGCGACTATCAGCCAC
TCACTATGCAGGTTTCGGGCGAATGGCCAGCGCAATTGAGCGGTAATTTATATCGTAATGGGCCAGCGAAAATGTCTCGG
GCAGGTAAGGCATATCAGCATTGGTTCGATGGCGATGGCATGGTGCAGCAGTTTAGTGTGGCTAATGGGCAAATTCAGCA
TCAGGCTAAGTTTATCCAAACAAAGAAGTTTAAAGTGGAGGAGGCCGCTGGCGCGTTTAAGTACACCACCGCAGGCACAG
TGATCCCCAATGCTTTACCTATTCGAAATAACGATGACTTGAATGTGGCTAACACCAGTTTATTACCGCGCCACGGTGAG
TTGCTTGCCTTGTGGGAAGCGGGTTCACCCTATCGACTCGATGCGCAGACCTTAACCACGCTCGGCGTTAAAAGCTTTGG
CGACAAGTTTAAGGGCTTACCCTTTTCCGCCCATCCACTCGATGATGGACAAGGTGGACTGTGGAATTTTGGCGTCTGGT
ATGTCGGCGGCGAGAAGCAATTACTTGTGTATCAAGTGGCAGCCGATGACAGCATTGCCAGAATGGAAGTGATTGAATTA
CCGCAGGCGAGTTACTTACATGCCTTCGCGCAGAGTGAGAATAAGCTAGTGTTTTATATTTCATCCTGCGTGTATGAAGA
GGGCGAAACCTATATCGATGCTTTTAAATGGCGACCCGAATTGCCATCGCAATTACTGGTTATCGACAAAGCCGACTTTA
AAGCGCGCCAGTTACACCCGCTGCCTGCTGGATTTGTGTTTCACTTTGGTCAAGCCATTGAGAAGAATGACGAACTGTCA
GTGCAACTTTGCCTCTATCCCAATTCGGCGATTTTAACTCAAGGGATGAAGGGGCTACTGACGGGCGCCCGAAAAACAAA
AACACCCCATGCCGAGTTAGTGACGATCACAGTACCTCTCACAGCACCACTTACAGCAGCTAGCAAAACGCAGTTTGCGG
AGACCTTGTCCTCCTCTGCACGTATTAACCGCAGCGGCGTGATGATGGAGTTTCCCCAGTTTGCGCAGCACACTATGAGT
AACAAAGAGTCTGCACAGCAAACGAGCAATGCTGAAAATCTTACAGAGAAAATGGGTGGGGCATCAGAGTTTGTCCCGTT
ATTTGGCGTCGGAGCAAGATCAGACTCTGTTGCTAATTCTGGTGCTAATTCTAACTTTAAATATGAAGCCGAATCTGAAT
CAGGACTGAGTAATACTCTGTATTGTATTCATAATACAAAAGACAGCAGCCAGCACGGTACGGATGAACTTGGCGACGCG
AGCTATAGCGCGTTTTATCTTGGCAAAGGCAAGATTGCCGAAGAGCCGCTCTATATCCCCGCCACTGAGCAGCATGAAGC
CTATTTGTTGATGACTTGGCTCGATTACCACAATGCACAATCTGGCTTGTCTTTGTTTCGTGCCAGTGACATCAGTGCGG
GACCCATTGCTTCGGCTCAGATGAACAGGGTATTGCCGTTAGGATTTCACGGTTGTTTCATTAACGCTTAA

Upstream 100 bases:

>100_bases
ACGCCGGCAGTTTTGGGCCTGCGAGCTTTAAAGACGCCAGCTTTAGTTTGCAGGCCGATGCGCAAATCACTATACATATT
CGCTAAGGGAGGGCTGCACC

Downstream 100 bases:

>100_bases
TATATTGACCTATAAACAAAAAACGCAGCCAAGGGCTGCGTTTTATTTCAGGCGAAAGCAGTGTTTAGTCTTCAATTTCC
GCATTGTGATAGACGTTTTG

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLAQDNAQAVAREPWAIGFSGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVANGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNDELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTAASKTQFAETLSSSARINRSGVMMEFPQFAQHTMS
NKESAQQTSNAENLTEKMGGASEFVPLFGVGARSDSVANSGANSNFKYEAESESGLSNTLYCIHNTKDSSQHGTDELGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAQMNRVLPLGFHGCFINA

Sequences:

>Translated_556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLAQDNAQAVAREPWAIGFSGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVANGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNDELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTAASKTQFAETLSSSARINRSGVMMEFPQFAQHTMS
NKESAQQTSNAENLTEKMGGASEFVPLFGVGARSDSVANSGANSNFKYEAESESGLSNTLYCIHNTKDSSQHGTDELGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAQMNRVLPLGFHGCFINA
>Mature_556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLAQDNAQAVAREPWAIGFSGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVANGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNDELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTAASKTQFAETLSSSARINRSGVMMEFPQFAQHTMS
NKESAQQTSNAENLTEKMGGASEFVPLFGVGARSDSVANSGANSNFKYEAESESGLSNTLYCIHNTKDSSQHGTDELGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAQMNRVLPLGFHGCFINA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=359, Percent_Identity=23.1197771587744, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI82617624, Length=359, Percent_Identity=23.1197771587744, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI74027270, Length=379, Percent_Identity=20.844327176781, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI4506591, Length=362, Percent_Identity=23.2044198895028, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564756, Length=373, Percent_Identity=23.5924932975871, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71998242, Length=419, Percent_Identity=22.673031026253, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24646669, Length=400, Percent_Identity=20.75, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 60662; Mature: 60662

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRQFLAGSAALMTTAVWASSVDKLLAQDNAQAVAREPWAIGFSGAEGDYQPLTMQVSG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCEEEEEECC
EWPAQLSGNLYRNGPAKMSRAGKAYQHWFDGDGMVQQFSVANGQIQHQAKFIQTKKFKVE
CCCCCCCCCEEECCCHHHHHCCHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHCEEEH
EAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGELLALWEAGSPYRLDAQTLTT
HHCCCEEEEECCCCCCCEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCEEECHHHHHH
LGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHEEHEECC
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHP
CCHHHHHHHHCCCCCEEEEEEEEHHCCCCCEEEHEECCCCCCCEEEEEECCCCCHHHCCC
LPAGFVFHFGQAIEKNDELSVQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPL
CCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCHHHHHCCCCCCCCCCCEEEEEEEEC
TAPLTAASKTQFAETLSSSARINRSGVMMEFPQFAQHTMSNKESAQQTSNAENLTEKMGG
CCCCCCCHHHHHHHHHHHCCEECCCCCEEECHHHHHHHHCCHHHHHHHCCHHHHHHHHCC
ASEFVPLFGVGARSDSVANSGANSNFKYEAESESGLSNTLYCIHNTKDSSQHGTDELGDA
CCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAQ
CEEEEEECCCCCCCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHEEECCCCCCCCCHHH
MNRVLPLGFHGCFINA
HCCCCEEEECCEEECC
>Mature Secondary Structure
MDRRQFLAGSAALMTTAVWASSVDKLLAQDNAQAVAREPWAIGFSGAEGDYQPLTMQVSG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCEEEEEECC
EWPAQLSGNLYRNGPAKMSRAGKAYQHWFDGDGMVQQFSVANGQIQHQAKFIQTKKFKVE
CCCCCCCCCEEECCCHHHHHCCHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHCEEEH
EAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGELLALWEAGSPYRLDAQTLTT
HHCCCEEEEECCCCCCCEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCEEECHHHHHH
LGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHEEHEECC
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHP
CCHHHHHHHHCCCCCEEEEEEEEHHCCCCCEEEHEECCCCCCCEEEEEECCCCCHHHCCC
LPAGFVFHFGQAIEKNDELSVQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPL
CCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCHHHHHCCCCCCCCCCCEEEEEEEEC
TAPLTAASKTQFAETLSSSARINRSGVMMEFPQFAQHTMSNKESAQQTSNAENLTEKMGG
CCCCCCCHHHHHHHHHHHCCEECCCCCEEECHHHHHHHHCCHHHHHHHCCHHHHHHHHCC
ASEFVPLFGVGARSDSVANSGANSNFKYEAESESGLSNTLYCIHNTKDSSQHGTDELGDA
CCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAQ
CEEEEEECCCCCCCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHEEECCCCCCCCCHHH
MNRVLPLGFHGCFINA
HCCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]