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The map label for this gene is yfiK [H]

Identifier: 126173479

GI number: 126173479

Start: 1427082

End: 1427732

Strand: Reverse

Name: yfiK [H]

Synonym: Sbal_1241

Alternate gene names: 126173479

Gene position: 1427732-1427082 (Counterclockwise)

Preceding gene: 126173480

Following gene: 126173478

Centisome position: 27.85

GC content: 48.54

Gene sequence:

>651_bases
ATGGATACGCAAAACAACATCATCAGTGTCGGCTTAGTAGAAGATCAACAATTGGTTCGCCAAGGTATCGCTAGCCTGCT
CGCCATTTCCGACAACATTCGCGTGGTGTGGCAGGCCGAAGACGGCCAAGATGCACTCAGCCAACTCGCTAATAATCCTG
TCGATGTGCTGCTCAGTGATATTCGCATGCCAAATCTTGATGGCATCGCCATGCTCAAACAGATACGTCAGAATGCCAAT
AGCTTACCTGTCATCATGTTAACCACCTTCGATGACAGTGAGTTATTCCTCAATAGTTTACAGGCGGGCGCCAACGGCTT
TTTGCTCAAGGACGTGTCACTCGATAAGTTACTGCACGCCATCGAAACCGTCGCTAAAGGCGGCTACTTGATTGAGCCTT
CGGTACTCAAACAGATGAGTGAGCCTAAACCTGCACCTAGCGTTGGGGTAACTAGAAATGAATTATTGTCGGAACGGGAG
CGGGAAATCCTCAGTTTTATGGCGGGCGGATTCTCCAACAAGGAAATCGCCAACGCGGTATTTTTAGCCGAGGGCACAGT
GAAGAATCATGTCTCTAACATACTCACTAAACTCGATTGCCGAGACCGCACTCAAGCTGTACTCAAGGGCTTACAACTGG
CATTGATCTAA

Upstream 100 bases:

>100_bases
AGGTGCAGCTAAGGGCGAATGACTCGGCGCCCGGCTGCCAACTCACCCTCACCCTCCCAGCACTCGCTTAACTTTTTACT
AAAAACGCTTTGGAATTTAT

Downstream 100 bases:

>100_bases
CCCAATAGCATTCAGAGTGACGACAGCAAAGCCCGTGCTCAACGGCCGCAATCAATCTTACCCAAACTGCATGAATTCAT
AGGAAAGGAACACTAATGCA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDTQNNIISVGLVEDQQLVRQGIASLLAISDNIRVVWQAEDGQDALSQLANNPVDVLLSDIRMPNLDGIAMLKQIRQNAN
SLPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLHAIETVAKGGYLIEPSVLKQMSEPKPAPSVGVTRNELLSERE
REILSFMAGGFSNKEIANAVFLAEGTVKNHVSNILTKLDCRDRTQAVLKGLQLALI

Sequences:

>Translated_216_residues
MDTQNNIISVGLVEDQQLVRQGIASLLAISDNIRVVWQAEDGQDALSQLANNPVDVLLSDIRMPNLDGIAMLKQIRQNAN
SLPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLHAIETVAKGGYLIEPSVLKQMSEPKPAPSVGVTRNELLSERE
REILSFMAGGFSNKEIANAVFLAEGTVKNHVSNILTKLDCRDRTQAVLKGLQLALI
>Mature_216_residues
MDTQNNIISVGLVEDQQLVRQGIASLLAISDNIRVVWQAEDGQDALSQLANNPVDVLLSDIRMPNLDGIAMLKQIRQNAN
SLPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLHAIETVAKGGYLIEPSVLKQMSEPKPAPSVGVTRNELLSERE
REILSFMAGGFSNKEIANAVFLAEGTVKNHVSNILTKLDCRDRTQAVLKGLQLALI

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=34.375, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=35.9223300970874, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786747, Length=193, Percent_Identity=33.160621761658, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788222, Length=193, Percent_Identity=32.1243523316062, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787473, Length=198, Percent_Identity=28.7878787878788, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788712, Length=192, Percent_Identity=26.5625, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=83, Percent_Identity=43.3734939759036, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=33.9622641509434, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=77, Percent_Identity=41.5584415584416, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693157, Length=88, Percent_Identity=38.6363636363636, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23547; Mature: 23547

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQNNIISVGLVEDQQLVRQGIASLLAISDNIRVVWQAEDGQDALSQLANNPVDVLLSD
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
IRMPNLDGIAMLKQIRQNANSLPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLHA
HCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHH
IETVAKGGYLIEPSVLKQMSEPKPAPSVGVTRNELLSEREREILSFMAGGFSNKEIANAV
HHHHHCCCEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FLAEGTVKNHVSNILTKLDCRDRTQAVLKGLQLALI
EEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTQNNIISVGLVEDQQLVRQGIASLLAISDNIRVVWQAEDGQDALSQLANNPVDVLLSD
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHH
IRMPNLDGIAMLKQIRQNANSLPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLHA
HCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHH
IETVAKGGYLIEPSVLKQMSEPKPAPSVGVTRNELLSEREREILSFMAGGFSNKEIANAV
HHHHHCCCEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FLAEGTVKNHVSNILTKLDCRDRTQAVLKGLQLALI
EEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]