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The map label for this gene is gltD [H]

Identifier: 126173415

GI number: 126173415

Start: 1355354

End: 1356760

Strand: Reverse

Name: gltD [H]

Synonym: Sbal_1177

Alternate gene names: 126173415

Gene position: 1356760-1355354 (Counterclockwise)

Preceding gene: 126173416

Following gene: 126173414

Centisome position: 26.46

GC content: 50.82

Gene sequence:

>1407_bases
ATGAGTAACGATTTTCAATTTATTGAAGTGGGTCGCAAAAACCCCACCAAACACCCTGCGGCTAAACGTGCGACTCAGTT
TATTGAGATTTATCAGCCTTTTGCTCAGCCTGAAGTGGCACAGCAAGCGGATCGCTGTTTAGATTGCGGCAATCCTTATT
GTGAGTGGAAATGTCCGCTACACAATTATATTCCTAACTGGCTCAAGCTAGCGGAGCAAGGTCGCATCATGGAAGCGGCG
GATTTAGTGCATGAGACCAACACTCTGCCCGAAATCTGCGGCCGTGTTTGTCCACAGGACAGACTCTGCGAAGGCGCTTG
TACCCTTAACGACGACTTTGGTGCTGTCACTATCGGTAATGTCGAAAAATACATTACCGATACAGCCATCGCCCAAGGCT
GGCGCCCAGATATGAGCAAGGTCACGCCGCGCGCTGAACGAGTAGCGATTGTCGGTGCAGGTCCTGCAGGGTTGGGCTGC
GCGGATATTCTGTCTCGTAACGGCATTAAAGCCGTGGTCTTTGATAAATATCCACAAATTGGTGGCCTACTCACCTACGG
TATTCCGTCTTTTAAACTCGACAAGGCCGTCATGGCGACCCGTCGCAGTGTATTAGAAGGCATGGGCATTGAGTTCAAAC
TCGGGGTTACTGTCGGTAAAGACATCCCATTTACTAAACTGCTCGAAGAGTACGATGCCGTATTCCTTGGTATGGGCACC
TATACCGCGATGAAAGCGGGCCTTGAGGGTGAAGATGCCCAAGGCGTATACCAAGCCCTGCCCTATTTGATTGGTAACAC
CCATCATTTAATGGGCACCCAGAATACTGAAACGCCCTACCTAAACCTTGAAGGTAAACGTGTGGTGGTATTAGGCGGTG
GTGATACTGCGATGGATTGCGTGCGCACAGCCGTCAGACAAGGTGCCAGCAGCGTAGTCTGTGCTTACCGCCGTGACGAG
GCCAACATGCCAGGTTCACGCCGCGAAGTGCAAAATGCGCGGGAGGAAGGCGTTAATTTCCTGTTTAACCGTCAACCTGT
GGCGATTAAAACCCAAGATGGCAAAGTAATTGGCATTGAATGCGTCGAAACCGCCATGGGTAAAGCCGATGCCAGCGGTC
GTCAACGCGCCGAAGCGATTGCGGGTAGTGAACAGTTGCTCGAAGCCGATGCGATTATTATTGCTTTCGGTTTCCAACCT
AGCCCAGCACCTTGGTTTGCCGACCATGGCATCGAGCTCGATCAATGGGGCCGAGTTAAAGCGAGTAAGTTAGACGAAAA
TCCTTTCCAAACTACAAACCCAAAAGTGTTTGCTGGTGGTGATATGGTGCGCGGTTCTGACTTAGTCGTGACAGCCATTG
CCGAAGGTCGCGATGCTGCTCAAGGTATTCTTAACTACTTAGACTAA

Upstream 100 bases:

>100_bases
TTGCTTCGTATTAGTAAAACCTAAAAATATTGCCGTGGCCGATCTACTCAAGTTAGAGAAATCGAGCCCAGAATTAGTTG
TAAAAGCGGGGTAATGGCGC

Downstream 100 bases:

>100_bases
GCGCTAAGCTTAAACGCCAATGTGTTGGTTTTTTCGCTGAAAAACTGTTTTACCGCTGTCGTTTAACAAGCACTGCAATG
GCTGGACTTAGGCAAACAAG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSNDFQFIEVGRKNPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGC
ADILSRNGIKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVIGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQP
SPAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Sequences:

>Translated_468_residues
MSNDFQFIEVGRKNPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAA
DLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGC
ADILSRNGIKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDE
ANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVIGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQP
SPAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
>Mature_467_residues
SNDFQFIEVGRKNPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPLHNYIPNWLKLAEQGRIMEAAD
LVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGNVEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCA
DILSRNGIKAVVFDKYPQIGGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGTY
TAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDCVRTAVRQGASSVVCAYRRDEA
NMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVIGIECVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPS
PAPWFADHGIELDQWGRVKASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=447, Percent_Identity=29.082774049217, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=65.5982905982906, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=460, Percent_Identity=59.7826086956522, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI87082180, Length=460, Percent_Identity=56.304347826087, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1788468, Length=445, Percent_Identity=27.8651685393258, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789243, Length=442, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=32.9243353783231, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=448, Percent_Identity=30.5803571428571, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17543860, Length=205, Percent_Identity=32.6829268292683, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=32.7083333333333, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=36.701030927835, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=36.701030927835, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640763, Length=471, Percent_Identity=27.3885350318471, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI18858217, Length=471, Percent_Identity=27.3885350318471, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50915; Mature: 50784

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFQFIEVGRKNPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPL
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAEQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCADILSRNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCC
GGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVVCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVIGIE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEHH
CVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPSPAPWFADHGIELDQWGRVK
HHHHHHCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
HHHCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNDFQFIEVGRKNPTKHPAAKRATQFIEIYQPFAQPEVAQQADRCLDCGNPYCEWKCPL
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCH
HNYIPNWLKLAEQGRIMEAADLVHETNTLPEICGRVCPQDRLCEGACTLNDDFGAVTIGN
HHHCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEEECC
VEKYITDTAIAQGWRPDMSKVTPRAERVAIVGAGPAGLGCADILSRNGIKAVVFDKYPQI
HHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCC
GGLLTYGIPSFKLDKAVMATRRSVLEGMGIEFKLGVTVGKDIPFTKLLEEYDAVFLGMGT
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCEEEEECCH
YTAMKAGLEGEDAQGVYQALPYLIGNTHHLMGTQNTETPYLNLEGKRVVVLGGGDTAMDC
HHHHHCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEECCCEEEEECCCCHHHHH
VRTAVRQGASSVVCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPVAIKTQDGKVIGIE
HHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEECCCCEEEEECCCCEEEHH
CVETAMGKADASGRQRAEAIAGSEQLLEADAIIIAFGFQPSPAPWFADHGIELDQWGRVK
HHHHHHCCCCCCHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
ASKLDENPFQTTNPKVFAGGDMVRGSDLVVTAIAEGRDAAQGILNYLD
HHHCCCCCCCCCCCEEEECCCEECCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]