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The map label for this gene is rlmA [H]

Identifier: 126173392

GI number: 126173392

Start: 1329514

End: 1330356

Strand: Direct

Name: rlmA [H]

Synonym: Sbal_1153

Alternate gene names: 126173392

Gene position: 1329514-1330356 (Clockwise)

Preceding gene: 126173391

Following gene: 126173395

Centisome position: 25.93

GC content: 44.01

Gene sequence:

>843_bases
ATGGGTCTCGAATATCAGTGTCCAACCTGCGGACTTGTGTTAAAACAACACAAAGCCTCCCAAGGTTTTTATTGTGTCAA
TAAGCATCACTTTGATCGGAATGAGCAAGGCTATTGGGTTTTTACTAAACCACAACGCCAAAAGCCGACTGGTGATTCGC
GCGAGCAAGTTAGAGCTAAGCGTTTTCTACTCGAATCAGGCATATTTTCGCCTTTGATCGATAAACTGGCTGAGATAATC
ACTGGATCTATTACGCCTGATGCTTGTTTATTGGATTATGAATGCGCTCAGGGCTTCTTCTTGCGTGCTTTAGCTTCCAA
GCTGGCTGAGGTCGAAAATCCGCTAAACGTCCAGTACGTGGGCGTAGCGGACGCAGAAAATGCCATTTTTGCTGCCGCGA
AGGCGCAGACTCCTGGGATCTTGTGTTTAAGCTCTTCTAAAAAATTACCCTTTATCGATAATTGTGTGGATTTTATTACT
GTTATTGATCGGCCGTTAAAGGGAAAAGAGTCTGTTAGGATATTGAAAGACGATGGGACCATGTTACTTGTTATGCCTGG
GCCACGACACCTATGGCAACTCAGGGAATATATTTATCCTGACTTGGCTGAAAAACCTTTCAAAGTGAATTTGCCCTCTG
CATTATCGGTAAAAGACACTCAGGCACTGAGCTTCACCTTATCTGTTACCGGTGAGCAGGCATTAACGCTATTGGATATG
GCGCCTTATGCGTGGCGTGCAACTGACAAGATGAAGCACGATATTCAAGCTAATCATTTTGAGTCGTTGGAAATTGACTA
TGTATTAGTCTCAGCACATAAACGCGTGGTTGAATCGCTATAG

Upstream 100 bases:

>100_bases
TCATAGTGAGATAATAAATAATAGAATCGGGTCATGTACCCGATTTTTGTTTTTGGAGCTTCCTCATTTTCTGAAGCTTA
ACTTATTTTTGGAGCTTTAT

Downstream 100 bases:

>100_bases
GTTAAAATCTGCAGTTGATGCGGTTAATTTTAAGCCAATTCATAGACTTAGTTCGATGGTTTTTAGATGCTTTAAAAGGC
TATATTACTCAAGTCTATGA

Product: ribosomal RNA large subunit methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MGLEYQCPTCGLVLKQHKASQGFYCVNKHHFDRNEQGYWVFTKPQRQKPTGDSREQVRAKRFLLESGIFSPLIDKLAEII
TGSITPDACLLDYECAQGFFLRALASKLAEVENPLNVQYVGVADAENAIFAAAKAQTPGILCLSSSKKLPFIDNCVDFIT
VIDRPLKGKESVRILKDDGTMLLVMPGPRHLWQLREYIYPDLAEKPFKVNLPSALSVKDTQALSFTLSVTGEQALTLLDM
APYAWRATDKMKHDIQANHFESLEIDYVLVSAHKRVVESL

Sequences:

>Translated_280_residues
MGLEYQCPTCGLVLKQHKASQGFYCVNKHHFDRNEQGYWVFTKPQRQKPTGDSREQVRAKRFLLESGIFSPLIDKLAEII
TGSITPDACLLDYECAQGFFLRALASKLAEVENPLNVQYVGVADAENAIFAAAKAQTPGILCLSSSKKLPFIDNCVDFIT
VIDRPLKGKESVRILKDDGTMLLVMPGPRHLWQLREYIYPDLAEKPFKVNLPSALSVKDTQALSFTLSVTGEQALTLLDM
APYAWRATDKMKHDIQANHFESLEIDYVLVSAHKRVVESL
>Mature_279_residues
GLEYQCPTCGLVLKQHKASQGFYCVNKHHFDRNEQGYWVFTKPQRQKPTGDSREQVRAKRFLLESGIFSPLIDKLAEIIT
GSITPDACLLDYECAQGFFLRALASKLAEVENPLNVQYVGVADAENAIFAAAKAQTPGILCLSSSKKLPFIDNCVDFITV
IDRPLKGKESVRILKDDGTMLLVMPGPRHLWQLREYIYPDLAEKPFKVNLPSALSVKDTQALSFTLSVTGEQALTLLDMA
PYAWRATDKMKHDIQANHFESLEIDYVLVSAHKRVVESL

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=268, Percent_Identity=29.1044776119403, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 31422; Mature: 31290

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLEYQCPTCGLVLKQHKASQGFYCVNKHHFDRNEQGYWVFTKPQRQKPTGDSREQVRAK
CCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
RFLLESGIFSPLIDKLAEIITGSITPDACLLDYECAQGFFLRALASKLAEVENPLNVQYV
HHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GVADAENAIFAAAKAQTPGILCLSSSKKLPFIDNCVDFITVIDRPLKGKESVRILKDDGT
EECCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCC
MLLVMPGPRHLWQLREYIYPDLAEKPFKVNLPSALSVKDTQALSFTLSVTGEQALTLLDM
EEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCHHEEHHHC
APYAWRATDKMKHDIQANHFESLEIDYVLVSAHKRVVESL
CCCCCCCHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHCC
>Mature Secondary Structure 
GLEYQCPTCGLVLKQHKASQGFYCVNKHHFDRNEQGYWVFTKPQRQKPTGDSREQVRAK
CCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
RFLLESGIFSPLIDKLAEIITGSITPDACLLDYECAQGFFLRALASKLAEVENPLNVQYV
HHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GVADAENAIFAAAKAQTPGILCLSSSKKLPFIDNCVDFITVIDRPLKGKESVRILKDDGT
EECCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCC
MLLVMPGPRHLWQLREYIYPDLAEKPFKVNLPSALSVKDTQALSFTLSVTGEQALTLLDM
EEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCHHEEHHHC
APYAWRATDKMKHDIQANHFESLEIDYVLVSAHKRVVESL
CCCCCCCHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]