Click here to switch to the map view.

The map label for this gene is potF [H]

Identifier: 126173349

GI number: 126173349

Start: 1274617

End: 1275717

Strand: Direct

Name: potF [H]

Synonym: Sbal_1108

Alternate gene names: 126173349

Gene position: 1274617-1275717 (Clockwise)

Preceding gene: 126173348

Following gene: 126173350

Centisome position: 24.86

GC content: 44.5

Gene sequence:

>1101_bases
ATGAAGCTATTTAATAAGATGACCACTCTAGCTCTGGTAACTGCGAGCGTATTAGCGAGCGCAGCGGCCCAAGCGGAAGA
AGTGGTTCGCGTGTATAACTGGTCAGATTATATCGCGGAAGATACCTTAGAAAACTTCAAGAAAGAAACGGGCATTCGGG
TAATTTACGATGTGTTCGATAGTAACGAAGTGCTTGAGGCTAAATTACTGTCTGGTCGTAGTGGCTACGATATCGTTGTC
CCTTCTAACCACTTTCTCGCTAAGCAAATCAAAGCGGGTGCTTTCAAACCTTTAGACCGCGCTAAGCTACCTAACTTCAA
AAATTTAAATCCAGCCCTGATGAAGCTACTTGAGAAAGCCGATCCGGGTAACCAGTATGCCGTGCCTTATTTATGGGGAA
CCAATGGTATTGGTTACAACATAGATAAAGTGAAAGCGGCTGTGGGTGAAGATGCGCCATTCAACTCAATGGAACTGATC
TTCAATCCTAAATATGCTGAAAAAATATCTAAGTGTGGCTTTGCTATGCTGGACTCTGCCGACGATATGGTGCCCCAAGC
ACTGATTTATTTAGGTTTAGATCCTAACAGTTCCAACCCAAGCGATTATGAAAAAGCCGGTGAGTTACTGGAAAAAATCC
GTCCTTACGTGACCTATTTCCACTCATCTCGCTATATTTCTGATTTAGCAAACGGTGACATTTGTGTGGCCTTTGGTTTC
TCTGGCGACGTATTCCAAGCTAAAGCACGTGCTGAAGAGGCGGGTAATGGCAATAAGATTGGTTACTCGATTCCAAAAGA
AGGCGCCAACCTGTGGTTTGATATGTTAGCTATCCCAGCCGATTCGACTAACGCAGATAATGCACTGACGCTGATTAACT
ATTTTCTCCGTCCAGAAGTCATAGCGCCTATCTCTAACTATGTGGCCTATGCTAACCCGAACGATCCTGCCCAACCTCTG
GTTGATGAGGCTATCCGCACCGATCCCGCGATTTATCCACCACAAGAAGTGTTAGATAAACTTTATATTGGTGAAATCCG
TCCTTTGAAAATCCAACGCGTATTAACCCGTGTTTGGACCAAAGTGAAGTCAGGACAATAG

Upstream 100 bases:

>100_bases
GGTGTAGCATTGGTCGAAAATAACAACAATATCCGGATAGCCCGGTGACACAATGTCAGAATTTGCTTAAGCAATTCTGC
TACCCGAGAAAGGAGAAAGC

Downstream 100 bases:

>100_bases
TCGATAAGGGGCAAGCCTAGCTTGCCCCTAGCTTGGCAGACTTGCTATGTCCAATCCTATGCATAGTTCATCCACGTATA
GGATTTGACACAGTTTGGTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLPNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Sequences:

>Translated_366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLPNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ
>Mature_366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLPNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=370, Percent_Identity=53.5135135135135, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=33.6283185840708, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787711, Length=355, Percent_Identity=23.3802816901408, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40486; Mature: 40486

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRAKLPNFKNLNPALMKLLEKA
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHC
DPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELIFNPKYAEKISKCGFAMLDSA
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHCCC
DDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
HHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEV
CCCHHHHHHHHHHCCCCCEECCCCCCCCHHHEEHHHEECCCCCCCCHHHHHHHHHHCCHH
IAPISNYVAYANPNDPAQPLVDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWT
HHCHHHCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVKSGQ
HHCCCC
>Mature Secondary Structure
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRAKLPNFKNLNPALMKLLEKA
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHC
DPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELIFNPKYAEKISKCGFAMLDSA
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHCCC
DDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
HHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEV
CCCHHHHHHHHHHCCCCCEECCCCCCCCHHHEEHHHEECCCCCCCCHHHHHHHHHHCCHH
IAPISNYVAYANPNDPAQPLVDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWT
HHCHHHCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVKSGQ
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]