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The map label for this gene is purR [H]

Identifier: 126173314

GI number: 126173314

Start: 1231593

End: 1232609

Strand: Reverse

Name: purR [H]

Synonym: Sbal_1071

Alternate gene names: 126173314

Gene position: 1232609-1231593 (Counterclockwise)

Preceding gene: 126173316

Following gene: 126173311

Centisome position: 24.04

GC content: 42.38

Gene sequence:

>1017_bases
ATGAAAGTCACTATCAACGACGTAGCTAAATATGCTGGAGTGTCTATCAAGACAGTATCCCGTGTCACAAATAACGAACC
TTCCGTGAAGCAGGCCACAGTTGACAAAGTGAACGAGGCGATAAAAGCGCTTAACTACCAACCGAATTTAGCGGCGAGAA
ACCTCGCGGGCACTAAATCCTATGCTATTGGTTTTATTTACGATAATCCAAACGCTTACTACATTATTGATATGCAAAAT
GGGATCTTGGCAGCCTGTAAAGACAAGGGCTATGAACTTGTCATCCATCCTTGCAATGCCAAATCAGAGACCATTTGTGA
CGAGCTTACAGCACTAGTCAAATATTCTAGGCTAGCGGGATTAGTACTGACACCTCCCTTGTCAGAAGATCCGAAAGTAC
TCAAAGCGCTTACTGAGATTGATGCTAACTATGTGCGCATTATTGCAGGTGAAAAGGACAACGATCAGAATGGATTGACT
ATTTTAGTCAACGATAAATTTGGTGCAGTTGAAATCACCCAGCATCTGATTGACTTAGGCCACAAGCATATTGCGTTTTT
AAGTGGGGATTTACACCATGAATCCACCAAAGAACGGTTACTTGGTTTTAAACAAGCATTAACAATAAATGGCCTAAAAC
TGAATGAAAACTACATAATTGAAGGGAAGTACTCATTTGAGTCTGGTGTTGACGGTGCGAATAAGCTTATTAAACTAAAA
CATCGTCCGACTGCGATTGTAGCTTGTAACGATGAAATTGCCGCTGGTGCACTTTTTGCTGCTCGTTTAGCGGGTTTAGA
TATCCCAGTAGACTTATCGATTGTCGGTTTCGAAGATAGCCCATTCTCACGTCAAACCTGGCCTAAACTGACCACAGTGC
ATCAGCCCAATGAGAAAATTGCCCAAATAGCCACTGAGCTGTTAATAGCTAATCGCCGTGAGCAAAAAATTGAGCAATTT
AAAGTGTTTACACCAGAGCCTGTGATTCGCGACTCTTCCGCGGTGCCGCACAAATAA

Upstream 100 bases:

>100_bases
AAATACTGATAAAAATGTAAGCTTGTAAATTGCTGGTAAAGAATTTACGTTCATAGAACAGTTAACCCTTCGCGACCTTA
GACAAAGAGTTTTTACATCA

Downstream 100 bases:

>100_bases
GCAATGTTGAGAGACAAAAAAACGCACTCGATTGAGTGCGTTTTTGTTATATAAAGCCAGTGACTTAATGCAGTTTTAAT
TTAGGCCTGTGCCAGCGCAG

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILAACKDKGYELVIHPCNAKSETICDELTALVKYSRLAGLVLTPPLSEDPKVLKALTEIDANYVRIIAGEKDNDQNGLT
ILVNDKFGAVEITQHLIDLGHKHIAFLSGDLHHESTKERLLGFKQALTINGLKLNENYIIEGKYSFESGVDGANKLIKLK
HRPTAIVACNDEIAAGALFAARLAGLDIPVDLSIVGFEDSPFSRQTWPKLTTVHQPNEKIAQIATELLIANRREQKIEQF
KVFTPEPVIRDSSAVPHK

Sequences:

>Translated_338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILAACKDKGYELVIHPCNAKSETICDELTALVKYSRLAGLVLTPPLSEDPKVLKALTEIDANYVRIIAGEKDNDQNGLT
ILVNDKFGAVEITQHLIDLGHKHIAFLSGDLHHESTKERLLGFKQALTINGLKLNENYIIEGKYSFESGVDGANKLIKLK
HRPTAIVACNDEIAAGALFAARLAGLDIPVDLSIVGFEDSPFSRQTWPKLTTVHQPNEKIAQIATELLIANRREQKIEQF
KVFTPEPVIRDSSAVPHK
>Mature_338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILAACKDKGYELVIHPCNAKSETICDELTALVKYSRLAGLVLTPPLSEDPKVLKALTEIDANYVRIIAGEKDNDQNGLT
ILVNDKFGAVEITQHLIDLGHKHIAFLSGDLHHESTKERLLGFKQALTINGLKLNENYIIEGKYSFESGVDGANKLIKLK
HRPTAIVACNDEIAAGALFAARLAGLDIPVDLSIVGFEDSPFSRQTWPKLTTVHQPNEKIAQIATELLIANRREQKIEQF
KVFTPEPVIRDSSAVPHK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=29.7297297297297, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787948, Length=316, Percent_Identity=31.3291139240506, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789068, Length=353, Percent_Identity=30.5949008498584, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789202, Length=341, Percent_Identity=29.3255131964809, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=30.0291545189504, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=29.2899408284024, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=26.9592476489028, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787906, Length=267, Percent_Identity=27.7153558052434, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789456, Length=331, Percent_Identity=26.8882175226586, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790715, Length=314, Percent_Identity=22.6114649681529, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790689, Length=300, Percent_Identity=25.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37186; Mature: 37186

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
YAIGFIYDNPNAYYIIDMQNGILAACKDKGYELVIHPCNAKSETICDELTALVKYSRLAG
EEEEEEEECCCEEEEEECCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKVLKALTEIDANYVRIIAGEKDNDQNGLTILVNDKFGAVEITQHLIDLG
EEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKHIAFLSGDLHHESTKERLLGFKQALTINGLKLNENYIIEGKYSFESGVDGANKLIKLK
CCEEEEEECCCCCHHHHHHHHHHHHHEEECCEEECCCEEEEEEECCCCCCCCCHHEEEEE
HRPTAIVACNDEIAAGALFAARLAGLDIPVDLSIVGFEDSPFSRQTWPKLTTVHQPNEKI
CCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCHHHH
AQIATELLIANRREQKIEQFKVFTPEPVIRDSSAVPHK
HHHHHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
YAIGFIYDNPNAYYIIDMQNGILAACKDKGYELVIHPCNAKSETICDELTALVKYSRLAG
EEEEEEEECCCEEEEEECCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKVLKALTEIDANYVRIIAGEKDNDQNGLTILVNDKFGAVEITQHLIDLG
EEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKHIAFLSGDLHHESTKERLLGFKQALTINGLKLNENYIIEGKYSFESGVDGANKLIKLK
CCEEEEEECCCCCHHHHHHHHHHHHHEEECCEEECCCEEEEEEECCCCCCCCCHHEEEEE
HRPTAIVACNDEIAAGALFAARLAGLDIPVDLSIVGFEDSPFSRQTWPKLTTVHQPNEKI
CCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCHHHH
AQIATELLIANRREQKIEQFKVFTPEPVIRDSSAVPHK
HHHHHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA