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The map label for this gene is mpl [H]

Identifier: 126172967

GI number: 126172967

Start: 812211

End: 813602

Strand: Direct

Name: mpl [H]

Synonym: Sbal_0719

Alternate gene names: 126172967

Gene position: 812211-813602 (Clockwise)

Preceding gene: 126172966

Following gene: 126172968

Centisome position: 15.84

GC content: 47.99

Gene sequence:

>1392_bases
ATGCACGTACATATTTTAGGAATTTGTGGAACCTTCATGGGTGGTCTAGCGTTATTGGCTAGGGCAAAAGGGCACAGAGT
GACGGGCTCGGATGCCAATGTCTATCCTCCAATGAGCACGCAACTAGAGGACCAAGGCATTGAGCTTATTCAAGGTTTTG
ATCCCAGCCAGCTGGGTGAAGCAGGGCATTATCCTGACTTAGTTGTCATTGGTAATGCCATGAGCCGTGGTAATCCTTGC
GTGGAAGCCGTGTTGAACATTGGCATCCCTTATACCTCTGGCCCGCAGTTTTTAGCCGATCATATTTTGCCTGAGCGTTG
GGTACTGGCAGTGTCTGGTACTCACGGTAAAACCTCGACTTCTAGTATGTTAGCTTGGATTTTAGATGATTGCGGTTATG
AGCCTGGGTTCTTAATCGGTGGCGTACCGCAAAACTTTGGTGTGTCGGCGCGTTTAGGCAAGACGGCTTTTTTTGTGGTT
GAGGCCGATGAATACGACAGTGCCTTCTTCGATAAACGTTCGAAATTCGTGCATTATCATCCACGTACCTTAGTGATCAA
CAATCTTGAGTTCGATCATGCCGATATTTTTGCCGATCTGGCCGCGATTCAAAAACAGTTTAATCATTTAGTTCGCACTG
TACCTGGCGAAGGAAAGATCGTTTGGCCCGCCGATTCCCATGCTGTTAAGCAGACGATTGATATGGGGTGCTGGAGCGAG
CAAGAAACCTATCATTTAGCCGAATCTGCCAACGGTTGGTATGGCAAAGCCTTAAGTGATGATTGCCATCGCTTTGAAGT
GTTTTTTGATGGCGAGTCCCAAGGTATTCTCGAATGGGATTTGATTGGTCAGCACAATATTGAAAATGCCATGATGGCCA
TTGCTGCCGCGCGTCATGTGGGCGTTAAGCCGAGCGCTGCAATTGAAGCCTTAGCTAAGTTTATGCCACCTAAACGTCGA
CTCGAATTACTCGGAACCGTTAACGGCGTGAGTGTATACGACGACTTTGCCCATCATCCAACGGCGATTGCGACCACGTT
AAAAGGCATGCGGGCTAAGGTTGGTCAGAGCAAGATTACTGTGGTACTCGAGCCGCGCTCAAATACCATGAAGAGCGGTG
TCCATAAAGACACTTTAGCTTTTTCTATGATGCAAGCCGACGAAGCCTTTTTATATCAAGCCGATACCATTGACTGGAAT
ATGAAAGAAGCCATGGAAGCGGCGGTGATCCCTGTGACTGTGTTACACCAAATTGATGATGTGGTCGCCAAAGTAACGGC
AACGGCCAAAGCGGGCGACACCATAGTGGTTATGAGTAATGGCGGTTTTGGCGGGTTACATAAGAAGCTGCTGGCCGCAT
TAGAGCTGCGTTCAACTGTAGAGTTGGTTTAA

Upstream 100 bases:

>100_bases
TGTCATTTGCGATTGCAGTGCTTTTCCCCGATTGTTACCTGCCAATCTGTCGCCCGATGGATTAGAATGGCGTTGAATTC
AAAACATCAGAGCGAGCGGT

Downstream 100 bases:

>100_bases
GATGAGCTACACAAAGTCGACAAAGGCAATTAGCTTAGCTTGGACGGGCGCCTCGGGCGCCCCCTATGGCCTTAAGTTGC
TCGAGTGTCTGTTGGTATCG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESANGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALELRSTVELV

Sequences:

>Translated_463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESANGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALELRSTVELV
>Mature_463_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGEAGHYPDLVVIGNAMSRGNPC
VEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
QETYHLAESANGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHVGVKPSAAIEALAKFMPPKRR
LELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKITVVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALELRSTVELV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=459, Percent_Identity=62.962962962963, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1786279, Length=458, Percent_Identity=26.2008733624454, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50370; Mature: 50370

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCC
AGHYPDLVVIGNAMSRGNPCVEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETYHLAESANGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHV
CCCEEHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GVKPSAAIEALAKFMPPKRRLELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALELRSTVELV
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEDQGIELIQGFDPSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCC
AGHYPDLVVIGNAMSRGNPCVEAVLNIGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILDDCGYEPGFLIGGVPQNFGVSARLGKTAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFNHLVRTVPGEGKIVWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETYHLAESANGWYGKALSDDCHRFEVFFDGESQGILEWDLIGQHNIENAMMAIAAARHV
CCCEEHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
GVKPSAAIEALAKFMPPKRRLELLGTVNGVSVYDDFAHHPTAIATTLKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
VVLEPRSNTMKSGVHKDTLAFSMMQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVTATAKAGDTIVVMSNGGFGGLHKKLLAALELRSTVELV
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]