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The map label for this gene is gcvH [H]

Identifier: 126172867

GI number: 126172867

Start: 703503

End: 703892

Strand: Direct

Name: gcvH [H]

Synonym: Sbal_0618

Alternate gene names: 126172867

Gene position: 703503-703892 (Clockwise)

Preceding gene: 126172866

Following gene: 126172868

Centisome position: 13.72

GC content: 50.26

Gene sequence:

>390_bases
ATGAGCAATATTCCGACAGAACTGAAATACGCCTCTTCACACGAATGGATCCGCAAGGAAGAAGACGGCAGCTACACAGT
AGGTATCACTGAGCACGCCCAAGAGCTGTTAGGTGATATGGTTTTCGTTGAACTCCCAGAAGTTGGCGACACTGTAACTG
CGGGTGAAGACTGTGCTGTAGCTGAATCAGTAAAAGCGGCGTCTGACATTTATGCCCCCATTTCTGGCGAAGTGATTGCC
GTGAACGAAGCGCTGGAAGATTCTCCAGAACTCGTTAATAGCAGCGCTTACGGCGAAGGCTGGTTCTTCCGCGTAATGCC
TTCTGACGAATCAGAAGTCGACGCCCTGCTGGATGCCGAAGGCTACCAAGCTGTAATCGACGAAGAATAA

Upstream 100 bases:

>100_bases
AAATGCGTAAAAAACGTGTGGCTGTTAGAGTGGTAGCGCCAAACTTTGTGCGCAACGGTAAACAAGCATTTTAAAAAAAA
CGGAACAAGGAACAAGAACA

Downstream 100 bases:

>100_bases
TCGTCGACGCTAACGAAGCCCTGAGGGGCTTCGTTTGTTTTAGCGCAAGATTGACATAACAACAGTCCGTTAATCAGTAC
CCTACAGTTCTGAATTTCGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGEDCAVAESVKAASDIYAPISGEVIA
VNEALEDSPELVNSSAYGEGWFFRVMPSDESEVDALLDAEGYQAVIDEE

Sequences:

>Translated_129_residues
MSNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGEDCAVAESVKAASDIYAPISGEVIA
VNEALEDSPELVNSSAYGEGWFFRVMPSDESEVDALLDAEGYQAVIDEE
>Mature_128_residues
SNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGEDCAVAESVKAASDIYAPISGEVIAV
NEALEDSPELVNSSAYGEGWFFRVMPSDESEVDALLDAEGYQAVIDEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=46.218487394958, Blast_Score=112, Evalue=1e-25,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=45.3781512605042, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=72.0930232558139, Blast_Score=201, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=44.1666666666667, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=44.1666666666667, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=39.2, Blast_Score=97, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=43.2, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13978; Mature: 13847

Theoretical pI: Translated: 3.55; Mature: 3.55

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGEDCAV
CCCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCEEECCCCCHH
AESVKAASDIYAPISGEVIAVNEALEDSPELVNSSAYGEGWFFRVMPSDESEVDALLDAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCC
GYQAVIDEE
CCEEEECCC
>Mature Secondary Structure 
SNIPTELKYASSHEWIRKEEDGSYTVGITEHAQELLGDMVFVELPEVGDTVTAGEDCAV
CCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCEEECCCCCHH
AESVKAASDIYAPISGEVIAVNEALEDSPELVNSSAYGEGWFFRVMPSDESEVDALLDAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCC
GYQAVIDEE
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA