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The map label for this gene is mdh [H]

Identifier: 126172858

GI number: 126172858

Start: 694627

End: 695562

Strand: Direct

Name: mdh [H]

Synonym: Sbal_0609

Alternate gene names: 126172858

Gene position: 694627-695562 (Clockwise)

Preceding gene: 126172856

Following gene: 126172860

Centisome position: 13.55

GC content: 48.93

Gene sequence:

>936_bases
ATGAAAGTTGCTGTACTTGGTGCTGCTGGTGGTATCGGCCAGGCTCTTGCCCTACTGTTAAAAACTCAACTGCCTGCGGG
TTCACATTTGTCTCTATATGATATCGCACCGGTTACTCCGGGTGTTGCGGTTGACTTGAGCCACATCCCTACTGCTGTTG
AAATCAAAGGTTTTGCTGGTGAAGATCCAACGCCTGCCCTCGTGGGTGCTGACGTTGTTCTAATTTCTGCTGGTGTTGCG
CGTAAGCCTGGTATGGATCGTTCAGATCTGTTCAATATCAATGCCGGTATCGTTCGCAACCTGATCGAAAAAGTAGCGGC
AACTTGCCCTACGGCTTTGGTTGGCATTATCACTAACCCAGTCAACACGACTGTCGCTATCGCTGCAGAAGTGATGAAAA
AAGCGGGTGTTTACGATAAGAACCGTCTGTTCGGTATCACGACATTAGACGTGATCCGTTCTGAAACCTTCATCGCCGAA
TTAAAAGGCCTGAATGTTGCTGACGTTAAAGTGAATGTGATCGGCGGCCACAGCGGCGTGACCATTCTGCCATTACTGTC
TCAAGTTGAAGGCGTGACTTTCACTGACGAAGAAGTGGCAAGTCTGACTACTCGCATCCAAAACGCGGGTACAGAAGTGG
TTGAAGCTAAAGCTGGTGGCGGTAGCGCGACTCTGTCTATGGGTCAAGCAGCTTGCCGTTTCGGCCTGTCTTTGGTTCGT
GGTCTGCAAGGCGAAGCTAACATCGTTGAATGCGCCTATGTTGACGGCGGTAGCGAGCACGCTGAATTCTTCGCACAACC
TGTGCTGTTAGGCAAAAACGGTATTGAAAAAGTACTGCCTTACGGTGAAGTGAGTGCATTCGAAGCTAACGCTCGTGATT
CTATGTTAGATACGCTGAAAGGCGATATCAAACTAGGCGTTGATTTCGTTAAATAA

Upstream 100 bases:

>100_bases
CGACTAAGGGATAATTGAAATTGTTGTGACATTCCAGTAATGTGACGCCAACGTAAATTAGATCCATGTCACACATATCC
GAGAAATAACGGAGATAACT

Downstream 100 bases:

>100_bases
TCTTTCGGATAATCTTTTTAGATTATGACGAATTAAAAAACGGAGCCATTGAGCTCCGTTTTTTTATGCCTGCGTTTTAC
ACTTTTTAAAGCTCATTCAG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKGLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKGLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAE
LKGLNVADVKVNVIGGHSGVTILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=55.7692307692308, Blast_Score=323, Evalue=1e-88,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=79.6774193548387, Blast_Score=497, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=57.1884984025559, Blast_Score=327, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.1587301587302, Blast_Score=279, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6320125, Length=345, Percent_Identity=36.8115942028986, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324446, Length=367, Percent_Identity=35.9673024523161, Blast_Score=179, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=54.1935483870968, Blast_Score=316, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=47.6038338658147, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44.6666666666667, Blast_Score=243, Evalue=8e-65,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32074; Mature: 32074

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAELKGLNVADVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCEEECEEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSHLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPTALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGITTLDVIRSETFIAELKGLNVADVKVNVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVTFTDEEVASLTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVR
CHHHHHHHHCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANIVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA