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The map label for this gene is emrB [H]

Identifier: 126172733

GI number: 126172733

Start: 546802

End: 548430

Strand: Direct

Name: emrB [H]

Synonym: Sbal_0483

Alternate gene names: 126172733

Gene position: 546802-548430 (Clockwise)

Preceding gene: 126172732

Following gene: 126172734

Centisome position: 10.66

GC content: 51.32

Gene sequence:

>1629_bases
ATGAGCGCAACTGGCTCTATCACTGCGTCTGCCGATATTGAGGCGCAGGGCATCAGTGTCCCTAACAAGCCTACTGATTA
TGAGCGTGGCAGTCGCCGCTCATGGATCGCGGTATTTGGTGGGTTGATCGGCGCCTTTATGGCGATCCTCGATATCCAAA
TCACCAATGCATCGATGAAGGAGATTCAAGGCAGCCTTGGGGCAACGCTCGAAGAAGGCAGTTGGATTTCAACCGCGTAT
CTGGTGGCTGAGATGATCGCGATCCCGCTTTCTGGCTGGCTCAGTACGGGATTGTCGGTCAGGCGTTATCTGTTATGGAC
CACGGCCGCTTTTATCTTTGCTTCCGTACTTTGTTCTATGGCGTGGAACTTAGAGGCCATGATCGCCTTTCGGGCGTTAC
AGGGCTTCTTTGGTGGTGCGCTTATTCCGCTGGCCTTTAGGTTGATCCTTGAGTTTTTACCCGATAACAAACGTGCCGTC
GGCATGGCGTTATTTGGCGTGACGGCGACCTTTGCGCCTTCAATCGGCCCGACCTTAGGCGGTTGGTTGACTGAGCAATT
TAGCTGGCATTATCTGTTTTATATCAATGTGCCACCGGGTTTGTTAGTCATGGCCATGTTGGCCTACGGTTTAGAGAAAC
AGTCGGTCGTTTGGGATAAGCTGAAGAATGTCGATCTGGCGGGCATAGTCACTATGGCGCTAGGTATGGGTTGCCTTGAA
GTGGTGCTGGAAGAAGGTAACCGCAAGGACTGGTTTGGCTCGGAGCTCATTCGTAATCTGGCCATTATTGCCGTAGTCAA
TCTGGTGTTGTTTGTTTGGATCCAGTTAAGGCGTAAAGAACCGTTAGTGAATTTGCGTCTGCTCGGTAAGCGTGATTTTG
TGCTCTCCACCGTGGCGTATTTTTTACTGGGGATGGCACTATTCGGCGCTATCTATTTGATCCCGCTTTATCTATCGCAA
GTCCATGATTACACACCGTTAGAGATTGGTGGCGTGATTATGTGGATGGGATTTCCGCAGCTATTGGTATTGCCCTTAGT
GCCGAAATTGATGGAGCGCTTCGATAGTCGATATCTCGCCGCCTTTGGTTTTTTAATGTTCGCCATTAGCTATTACATGA
ATAGTCAGATGACCGCAGATTATGCCGGACCGCAGATGATCGCCTCGCAAGTGGTGCGGGCCTTAGGTCAGCCGTTTATT
CTGGTGCCTATTGGTATGCTTGCGACCATGCATTTGAAACCCCATGAAAATGCTTCGGCGTCGACGGTATTAAACGTAAT
GCGTAACTTAGGCGGCGCTTTTGGTATTGCGCTGGTCGCGACCCTATCAGACACGCTGGCGCGCGGGCATTTGGCCCATA
TTAAGGAATCCTTACCCGCAGTGAGCACGCAGGCACAGGACTATTTAGCCCAAAGCGCAAGCATGTTACAGGCGGGTGGT
TCAGATCCCTATACCGCCGCCGCGCAGGCGAAAGCTTTGTTGGGCAAAACCATGAGTCAGCAAGCCTATATTCAGGCCTA
TAACGATGTGTTTTACATCATGGGCGCTCTGCTACTCATTGCTGTGTTCGCCGTGCTGTTGATCCGTAAACCGACTGGGC
CTGTGGCCCACGATGCCATGGTCGAGTAA

Upstream 100 bases:

>100_bases
GAAGAAGCACACATGCTACCGGGCTTAAGTGCGGTCGTGAAAGTGGATACGGCGTCGGGTACTGCAATATCGGCAATCGC
TGCAAATACAGGTCGTTAGT

Downstream 100 bases:

>100_bases
TAACGTTTTTCGTTATCCTGTTGTCGCCAGAGATTTCCCCAGATCTATTGGTTTGCACAACAGCTTTGCGCCTTAGTCCT
CGCAGATTAAGGCGCTTTTT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSATGSITASADIEAQGISVPNKPTDYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAY
LVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASVLCSMAWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPDNKRAV
GMALFGVTATFAPSIGPTLGGWLTEQFSWHYLFYINVPPGLLVMAMLAYGLEKQSVVWDKLKNVDLAGIVTMALGMGCLE
VVLEEGNRKDWFGSELIRNLAIIAVVNLVLFVWIQLRRKEPLVNLRLLGKRDFVLSTVAYFLLGMALFGAIYLIPLYLSQ
VHDYTPLEIGGVIMWMGFPQLLVLPLVPKLMERFDSRYLAAFGFLMFAISYYMNSQMTADYAGPQMIASQVVRALGQPFI
LVPIGMLATMHLKPHENASASTVLNVMRNLGGAFGIALVATLSDTLARGHLAHIKESLPAVSTQAQDYLAQSASMLQAGG
SDPYTAAAQAKALLGKTMSQQAYIQAYNDVFYIMGALLLIAVFAVLLIRKPTGPVAHDAMVE

Sequences:

>Translated_542_residues
MSATGSITASADIEAQGISVPNKPTDYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAY
LVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASVLCSMAWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPDNKRAV
GMALFGVTATFAPSIGPTLGGWLTEQFSWHYLFYINVPPGLLVMAMLAYGLEKQSVVWDKLKNVDLAGIVTMALGMGCLE
VVLEEGNRKDWFGSELIRNLAIIAVVNLVLFVWIQLRRKEPLVNLRLLGKRDFVLSTVAYFLLGMALFGAIYLIPLYLSQ
VHDYTPLEIGGVIMWMGFPQLLVLPLVPKLMERFDSRYLAAFGFLMFAISYYMNSQMTADYAGPQMIASQVVRALGQPFI
LVPIGMLATMHLKPHENASASTVLNVMRNLGGAFGIALVATLSDTLARGHLAHIKESLPAVSTQAQDYLAQSASMLQAGG
SDPYTAAAQAKALLGKTMSQQAYIQAYNDVFYIMGALLLIAVFAVLLIRKPTGPVAHDAMVE
>Mature_541_residues
SATGSITASADIEAQGISVPNKPTDYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYL
VAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASVLCSMAWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPDNKRAVG
MALFGVTATFAPSIGPTLGGWLTEQFSWHYLFYINVPPGLLVMAMLAYGLEKQSVVWDKLKNVDLAGIVTMALGMGCLEV
VLEEGNRKDWFGSELIRNLAIIAVVNLVLFVWIQLRRKEPLVNLRLLGKRDFVLSTVAYFLLGMALFGAIYLIPLYLSQV
HDYTPLEIGGVIMWMGFPQLLVLPLVPKLMERFDSRYLAAFGFLMFAISYYMNSQMTADYAGPQMIASQVVRALGQPFIL
VPIGMLATMHLKPHENASASTVLNVMRNLGGAFGIALVATLSDTLARGHLAHIKESLPAVSTQAQDYLAQSASMLQAGGS
DPYTAAAQAKALLGKTMSQQAYIQAYNDVFYIMGALLLIAVFAVLLIRKPTGPVAHDAMVE

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=30.1010101010101, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1788710, Length=500, Percent_Identity=30.2, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=25.462962962963, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790195, Length=330, Percent_Identity=25.4545454545455, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=339, Percent_Identity=25.6637168141593, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=177, Percent_Identity=24.2937853107345, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=24.6666666666667, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59012; Mature: 58881

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATGSITASADIEAQGISVPNKPTDYERGSRRSWIAVFGGLIGAFMAILDIQITNASMK
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EIQGSLGATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASVLCSM
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPDNKRAVGMALFGVTATFAPSIGPTLG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHH
GWLTEQFSWHYLFYINVPPGLLVMAMLAYGLEKQSVVWDKLKNVDLAGIVTMALGMGCLE
HHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVLEEGNRKDWFGSELIRNLAIIAVVNLVLFVWIQLRRKEPLVNLRLLGKRDFVLSTVAY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
FLLGMALFGAIYLIPLYLSQVHDYTPLEIGGVIMWMGFPQLLVLPLVPKLMERFDSRYLA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AFGFLMFAISYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLATMHLKPHENASA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHEECCCCCCCHH
STVLNVMRNLGGAFGIALVATLSDTLARGHLAHIKESLPAVSTQAQDYLAQSASMLQAGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
SDPYTAAAQAKALLGKTMSQQAYIQAYNDVFYIMGALLLIAVFAVLLIRKPTGPVAHDAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
VE
CC
>Mature Secondary Structure 
SATGSITASADIEAQGISVPNKPTDYERGSRRSWIAVFGGLIGAFMAILDIQITNASMK
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
EIQGSLGATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASVLCSM
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPDNKRAVGMALFGVTATFAPSIGPTLG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHH
GWLTEQFSWHYLFYINVPPGLLVMAMLAYGLEKQSVVWDKLKNVDLAGIVTMALGMGCLE
HHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVLEEGNRKDWFGSELIRNLAIIAVVNLVLFVWIQLRRKEPLVNLRLLGKRDFVLSTVAY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
FLLGMALFGAIYLIPLYLSQVHDYTPLEIGGVIMWMGFPQLLVLPLVPKLMERFDSRYLA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AFGFLMFAISYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLATMHLKPHENASA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHEECCCCCCCHH
STVLNVMRNLGGAFGIALVATLSDTLARGHLAHIKESLPAVSTQAQDYLAQSASMLQAGG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
SDPYTAAAQAKALLGKTMSQQAYIQAYNDVFYIMGALLLIAVFAVLLIRKPTGPVAHDAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
VE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]