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The map label for this gene is rcfB [H]

Identifier: 125625266

GI number: 125625266

Start: 2466928

End: 2467611

Strand: Reverse

Name: rcfB [H]

Synonym: llmg_2512

Alternate gene names: 125625266

Gene position: 2467611-2466928 (Counterclockwise)

Preceding gene: 125625267

Following gene: 125625265

Centisome position: 97.55

GC content: 31.14

Gene sequence:

>684_bases
ATGAGTTTTAAAATGAAAGAAATGGCAAACGTAAACATTGAATATCTAATCAATACACTGGAACAAAAAAAAGTGAGTGT
TGTAACACGCAAAAAACATAGTTATATCATGTATCAAGGGATTGAATCAGAATATATCTATGTACTCAAAGATGGTGTAG
CGAAGATTAGCAATATTTTAAGAGATGGTCGTGAATTTAATATTGCTTATGTTGCGGAGCCAGACTTTGTTTCTTTATTG
GAAGAGAAACAAAACGATGGAATTTCAGCATTATTTAATGTACGAATTGAGTCTCCAACAGCCAGTTTTTACAAAATTTC
ACGCAGTGATTTTTGGAATTGGGTTCGTGAGGATTTGAATTTATTCAGAGTTGTTGATGACTTTTATAAACGAAGACTAG
CACTTAATTTAGAAATTCTTCAAAAGATGACAATCAATGGTAAGAAGGGAGCGGTTTGCGCTTGCCTTCACAGTTTGATT
GATGATTTCGGAATAAGAAAAAAAGATGGAATTCTGATTGATTTTACCGTCACTAATGAAGATATTGCAGGTTTTTGTGG
TATTTCTACACGAAATAGTGTTAACCGTATTCTTCATGATTTAAAGGATGAAAAAGTAATTGGAGTGATTGATAATAAAA
TTATGATTTATAATCCTCAATACTTAGAAGAATATATTAGTTAA

Upstream 100 bases:

>100_bases
GCTCTGAAGTTTGATTTAAATGATTTACATAAAACATGTTATAATAAAGGGGTTACAGCCCTGTATATGGCGAAATAAAT
GAATAAAAAATAGCGAGTAG

Downstream 100 bases:

>100_bases
TATAAATAAATAAAAAAGCTACTTTAAGTAGCTTTTTTGCTATAATTAAGTCAGCGTGATATCGAAATATTCAAGGAGGT
AAAATGGAAAATTTAAAAAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSFKMKEMANVNIEYLINTLEQKKVSVVTRKKHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAEPDFVSLL
EEKQNDGISALFNVRIESPTASFYKISRSDFWNWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLI
DDFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIGVIDNKIMIYNPQYLEEYIS

Sequences:

>Translated_227_residues
MSFKMKEMANVNIEYLINTLEQKKVSVVTRKKHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAEPDFVSLL
EEKQNDGISALFNVRIESPTASFYKISRSDFWNWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLI
DDFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIGVIDNKIMIYNPQYLEEYIS
>Mature_226_residues
SFKMKEMANVNIEYLINTLEQKKVSVVTRKKHSYIMYQGIESEYIYVLKDGVAKISNILRDGREFNIAYVAEPDFVSLLE
EKQNDGISALFNVRIESPTASFYKISRSDFWNWVREDLNLFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLID
DFGIRKKDGILIDFTVTNEDIAGFCGISTRNSVNRILHDLKDEKVIGVIDNKIMIYNPQYLEEYIS

Specific function: It is involved in the activation of genes necessary for anaerobic respiration [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26304; Mature: 26172

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKMKEMANVNIEYLINTLEQKKVSVVTRKKHSYIMYQGIESEYIYVLKDGVAKISNIL
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHH
RDGREFNIAYVAEPDFVSLLEEKQNDGISALFNVRIESPTASFYKISRSDFWNWVREDLN
HCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCHHEEEHHHHHHHHHHHHHH
LFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLIDDFGIRKKDGILIDFTVTNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DIAGFCGISTRNSVNRILHDLKDEKVIGVIDNKIMIYNPQYLEEYIS
CCCEECCCCCHHHHHHHHHHCCCCCEEEEECCEEEEECHHHHHHHCC
>Mature Secondary Structure 
SFKMKEMANVNIEYLINTLEQKKVSVVTRKKHSYIMYQGIESEYIYVLKDGVAKISNIL
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHH
RDGREFNIAYVAEPDFVSLLEEKQNDGISALFNVRIESPTASFYKISRSDFWNWVREDLN
HCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCHHEEEHHHHHHHHHHHHHH
LFRVVDDFYKRRLALNLEILQKMTINGKKGAVCACLHSLIDDFGIRKKDGILIDFTVTNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
DIAGFCGISTRNSVNRILHDLKDEKVIGVIDNKIMIYNPQYLEEYIS
CCCEECCCCCHHHHHHHHHHCCCCCEEEEECCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377 [H]