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The map label for this gene is ywjF [H]

Identifier: 125625250

GI number: 125625250

Start: 2451026

End: 2451907

Strand: Reverse

Name: ywjF [H]

Synonym: llmg_2494

Alternate gene names: 125625250

Gene position: 2451907-2451026 (Counterclockwise)

Preceding gene: 125625251

Following gene: 125625248

Centisome position: 96.93

GC content: 40.59

Gene sequence:

>882_bases
ATGTCAAAAATTGCATTTATTGGTACAGGCGTGATGGGAGCTGCTATGGCAGGTCATCTGATGGACGCTGGGCATGATTT
AATTGTCTATAATCGTACAAAATCAAAAACGGATGGTCTTGTAGCGCGTGGGGCGGCTTATGCTGAAAATCCACAAAAGG
CCGCAGCCCAAGCCGATTTTGTCATTACAATTGTTGGATATCCTAAGGATGTTCGCGAAGTTTATTTTGGTACAGAAGGA
ATTTTTAAAAATGATTGTTCAGGAAAAATTCTTATCGATATGACCACAACTGAACCGACTTTGGCTCAAGAAATTTATCA
AGAAGCCAAAAAAGCTGGTGCCTCTGCACTTGATGCTCCTGTTTCTGGTGGAGATTTGGGGGCCAAAAATGCGACATTGA
CCATTATGGTTGGTGGTGATTTGGCAACTTATGAAAAAGCTTTGCCTTTGTTTGAAAAAATGGGAAAAACAATCACTCAT
CAAGGTGTGGCTGGGAGTGGTCAACATACCAAAATGGCTAATCAAATTTGTATTGCAGGAACAATGACTGGAATGACAGA
GCTTTTAGCTTATGCCCAAAAAGCTGGATTAGATTTGGATAAAGTTTTAAATACTGTTGGAGGAGGTTCAGGTGCCACTT
GGTCTTTAGCTAATTATGGTCCTCGTATTCTCCGTGAAGATTATAGTGCTGGATTTTTTGTTAAGCATTTCATCAAGGAC
TTAGGGATTGCACTTGCTGAGGCTGATAAAATGAATCTTGAAATTCCAGCAACTAAAGGGGCAAAGGAACTTTATGAGCA
GTTAGCTGACAAAGGTTTTGAAAATGATGGAACACAAGCTTTGATTAAACTTTGGTGGCCAGAGGGACATCGCCCAGAAT
GA

Upstream 100 bases:

>100_bases
GAAAATCAAACGTTTGCGGGGTGATAAAGCCCGTAAACGTCGTGATTACCAAAACTAAATTAAACAGATTTTAGAAGTCT
GATAAATAAAAGGAGAGCTT

Downstream 100 bases:

>100_bases
TAAAGTCAAAATAAAAATTAAGCCTGATTATTCAGGCTTTTTTTACAGATATCAAAAAACAGGATACTGTCAATAATTAT
GTGTAAACACTCAAGTAGAG

Product: putative 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGAAYAENPQKAAAQADFVITIVGYPKDVREVYFGTEG
IFKNDCSGKILIDMTTTEPTLAQEIYQEAKKAGASALDAPVSGGDLGAKNATLTIMVGGDLATYEKALPLFEKMGKTITH
QGVAGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
LGIALAEADKMNLEIPATKGAKELYEQLADKGFENDGTQALIKLWWPEGHRPE

Sequences:

>Translated_293_residues
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGAAYAENPQKAAAQADFVITIVGYPKDVREVYFGTEG
IFKNDCSGKILIDMTTTEPTLAQEIYQEAKKAGASALDAPVSGGDLGAKNATLTIMVGGDLATYEKALPLFEKMGKTITH
QGVAGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
LGIALAEADKMNLEIPATKGAKELYEQLADKGFENDGTQALIKLWWPEGHRPE
>Mature_292_residues
SKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGAAYAENPQKAAAQADFVITIVGYPKDVREVYFGTEGI
FKNDCSGKILIDMTTTEPTLAQEIYQEAKKAGASALDAPVSGGDLGAKNATLTIMVGGDLATYEKALPLFEKMGKTITHQ
GVAGSGQHTKMANQICIAGTMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKDL
GIALAEADKMNLEIPATKGAKELYEQLADKGFENDGTQALIKLWWPEGHRPE

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=297, Percent_Identity=31.6498316498317, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI40556376, Length=265, Percent_Identity=29.0566037735849, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1786719, Length=281, Percent_Identity=36.6548042704626, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI145693186, Length=271, Percent_Identity=35.0553505535055, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1790315, Length=268, Percent_Identity=30.2238805970149, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=25.3521126760563, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=279, Percent_Identity=34.0501792114695, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24655230, Length=282, Percent_Identity=37.2340425531915, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI19922568, Length=282, Percent_Identity=37.2340425531915, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=23.9285714285714, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31156; Mature: 31025

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGAAYAENPQKAAAQADF
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCCHHHCCCHHHHCCCCE
VITIVGYPKDVREVYFGTEGIFKNDCSGKILIDMTTTEPTLAQEIYQEAKKAGASALDAP
EEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHCCC
VSGGDLGAKNATLTIMVGGDLATYEKALPLFEKMGKTITHQGVAGSGQHTKMANQICIAG
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHCCEEEEH
TMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHCCHHHHHHCCCCCHHHHHHHHH
LGIALAEADKMNLEIPATKGAKELYEQLADKGFENDGTQALIKLWWPEGHRPE
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SKIAFIGTGVMGAAMAGHLMDAGHDLIVYNRTKSKTDGLVARGAAYAENPQKAAAQADF
CCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECCCHHHCCCHHHHCCCCE
VITIVGYPKDVREVYFGTEGIFKNDCSGKILIDMTTTEPTLAQEIYQEAKKAGASALDAP
EEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHCCC
VSGGDLGAKNATLTIMVGGDLATYEKALPLFEKMGKTITHQGVAGSGQHTKMANQICIAG
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHCCEEEEH
TMTGMTELLAYAQKAGLDLDKVLNTVGGGSGATWSLANYGPRILREDYSAGFFVKHFIKD
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHCCHHHHHHCCCCCHHHHHHHHH
LGIALAEADKMNLEIPATKGAKELYEQLADKGFENDGTQALIKLWWPEGHRPE
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]