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The map label for this gene is glnA [H]

Identifier: 125625240

GI number: 125625240

Start: 2439405

End: 2440745

Strand: Reverse

Name: glnA [H]

Synonym: llmg_2484

Alternate gene names: 125625240

Gene position: 2440745-2439405 (Counterclockwise)

Preceding gene: 125625241

Following gene: 125625238

Centisome position: 96.49

GC content: 39.82

Gene sequence:

>1341_bases
ATGACAATCACAGCAGCAGACATTCGTCGCGACGTCAAAGAAAAGGATATCAAATTCTTGCGTTTGATGTTTACAGACAT
TCTTGGAACACTTAAAAACGTTGAAGTTCCAGCAACAGATGAACAATTGGACAAATTATTTGAAAACAAAATGATGTTTG
ATGGTTCGTCAATTGAAGGATTCGTTCGTATCAATGAATCAGACATGTATCTTTATCCAGACCTTGACACTTGGATTGTT
TTTCCTTGGGGTGACGAGTATGGTAAAGTCGCAGGTGTAATTTGTGATGTTTACACTCCAGAAGGTGAACCTTTCGCTGG
AGACCCACGTGGTGTCTTGAAACGTAACCTTAAATCAATGGAAAAATTAGGTTTTAAGAACTTTAACCTTGGACCTGAGC
CTGAGTTCTTCCTTTTCAAACTTAATGAAAATGACGAACCAACGCTTGAAGTAAATGACAAAGGTGGATATTTTGACCTT
GCCCCAACAGACCTTGCAGGAAATACAAGACGTGAAATCGTTAATGTATTAACAGATCTTGGATTTGAAGTGGAAGCTTC
TCACCATGAGGTAGCTATCGGTCAACATGAAATTGACTTTAAATACACTAATGCTTTAAAAGCGTGTGATAATATTCAAA
TCTTTAAACTTGTTGTTAAAACAATTGCGCGTAAACATGGTTTACATGCAACGTTCATGGCAAAACCAGTTCATGGAATA
AATGGTTCTGGAATGCACTGTAACATGTCTTTATTTACTGAAGATGGTGCCAATGCTTTTGCTGATCCAGCTGGAGATAT
GGGACTTTCAGATGTTGCTCATAGCTTTATCGCCGGACTTTTGAAACATGCTTATAACTTTACAGCCATCACAAACCCAA
CAGTTAACTCATACAAACGTTTGGTTCCTGGCTATGAAGCCCCAGTTTATGTTGCTTGGGCTGGACGTAACAGATCGCCT
TTGATTCGCGTTCCTGCTTCACGTGGCTTGTCAACTCGTGTAGAACTTCGTGCCGTTGACCCAACAGCAAATCCATATCT
TGCTTTGGCTGTTCTTTTGGCAGCAGGTCTTGACGGTGTTGAAAATAAATTGGAAGCACCAGAAGCAATTGAATCAAACA
TCTATGTGATGACTGAAGAAGAACGCAAAGCACATGGAATTACAGATTTGCCTTCTACTTTACACAATGCGGTTAAGGCT
TTGCGCGAAGACACAATTGTAACGGAAGCACTGGGCGAACACGTTTTGGTTAACTTTGTTGATGCGAAGCGTATCGAATG
GGCAAGCTATGCACAATTTGTATCGCAATGGGAAATTGATAATTACTTAGAACTTTACTAA

Upstream 100 bases:

>100_bases
ACTCCTAATCCCACTCATTTTACCCAACCGCGGATGTAAATTTGGTTGACATAATTTGTCAAGCAAGTTTACAGTGAAAA
TTTAACTAGGAGAGTAAAGC

Downstream 100 bases:

>100_bases
TATATAAATATATAAAAAACACTGACAATGTCTGTCAGTGTTTTTAGTTCATACAATTTTTTAATCAATTTCAGTAATTG
TCAAGACGTTGAAAGCAGTT

Product: GlnA protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKNFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDL
APTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYTNALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
NGSGMHCNMSLFTEDGANAFADPAGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSP
LIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVENKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKA
LREDTIVTEALGEHVLVNFVDAKRIEWASYAQFVSQWEIDNYLELY

Sequences:

>Translated_446_residues
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKNFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDL
APTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYTNALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
NGSGMHCNMSLFTEDGANAFADPAGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSP
LIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVENKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKA
LREDTIVTEALGEHVLVNFVDAKRIEWASYAQFVSQWEIDNYLELY
>Mature_445_residues
TITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIVF
PWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKNFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLA
PTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYTNALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGIN
GSGMHCNMSLFTEDGANAFADPAGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSPL
IRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVENKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKAL
REDTIVTEALGEHVLVNFVDAKRIEWASYAQFVSQWEIDNYLELY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=457, Percent_Identity=27.3522975929978, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI1790301, Length=447, Percent_Identity=40.4921700223714, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=27.1929824561404, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49743; Mature: 49612

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCE
FVRINESDMYLYPDLDTWIVFPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSM
EEEECCCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EKLGFKNFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLAPTDLAGNTRREIVNVLTDL
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAIGQHEIDFKYTNALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
CCEEECCCCEEEECCEECCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NGSGMHCNMSLFTEDGANAFADPAGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKR
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
LVPGYEAPVYVAWAGRNRSPLIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGV
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCH
ENKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKALREDTIVTEALGEHVLVNFV
HHHCCCCHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEH
DAKRIEWASYAQFVSQWEIDNYLELY
HHHHCCHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
TITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCE
FVRINESDMYLYPDLDTWIVFPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSM
EEEECCCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EKLGFKNFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLAPTDLAGNTRREIVNVLTDL
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAIGQHEIDFKYTNALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
CCEEECCCCEEEECCEECCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NGSGMHCNMSLFTEDGANAFADPAGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKR
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
LVPGYEAPVYVAWAGRNRSPLIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGV
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCH
ENKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKALREDTIVTEALGEHVLVNFV
HHHCCCCHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEH
DAKRIEWASYAQFVSQWEIDNYLELY
HHHHCCHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]