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The map label for this gene is ywfF [H]

Identifier: 125625220

GI number: 125625220

Start: 2413060

End: 2414601

Strand: Reverse

Name: ywfF [H]

Synonym: llmg_2464

Alternate gene names: 125625220

Gene position: 2414601-2413060 (Counterclockwise)

Preceding gene: 125625221

Following gene: 125625219

Centisome position: 95.46

GC content: 37.87

Gene sequence:

>1542_bases
ATGATAAATTTAAAAATAGGACAGAAACTTCAACTCGAAATCGAGCGAATGGGGATAAATGGTGAAGGGATTGGTGTCAT
TTCAGGACGACTTGTCTTTATTCCTTATGCGCTTCCTGGTGAAGAAGTGTTAGTTGAAATTACTGAAAATGCTAGAAATT
TCAGTCGTGCAAAATTGGTTAAGATTATTGAAAAATCACCAAATCGTGTGAAACCGCAGGATAGAGCTTATCATGAAATG
AGTCAATCTCACATCATGCATCTTTCTTATCCAATGCAATTGGAATTCAAACGGGATGTTATGCGTCAAGCTTTGGAAAA
ATACAAACCAGCAGGATGGCGTAATTATGAATTGCGCGCGACTTTGGGAATGGAAAATACGCTTGGATATCGTAACAAGC
TCCAGTTCCAAGTACGTCGTTTGAATGATGGAACGGTAATTGCTGGACTTTATCAAGAAGGAACACATCACTTGGTAAAC
CTAGATAACTGTTTGGTTCAAGATCCGAAAATCCAAGAGATTATCAATCACATTTGTCGTTTGATTGAAAAATTTGATTT
GCCAGTTTATGATGAGCGTAAAATTGGCGGAATTCGTACGGTCATGGTTCGTCGTTCACAAAAAACAGGTGAAGTACAAA
TTATTTTTGTGACTTCAACACCAATTATTTTAGATGGACAAGTTTGGCCTGAATTACGTGAAGAAGCTTCAAAACGTCAA
AAAAATTCTTTTGTTAAATTTGACAAAATGTTGTCAGCGCTGACAGAAGAATTTGAAGAAATTGTGACGGTTGCGGTCAA
TTTCCACCCAAAAAAAACCAGTGAAATCTATGGCGAACGCACACAAGTTTTGTTCAATGAAAAAGAAACAATTACCGAAG
GGGTCTTGGACTATGAGTTTGAATTAAGTCCACGTGCTTTCTATCAATTGAACTCTGAGCAAGCAAATGTACTTTATGGT
GAAGCAGTTAAGGCTTTGAATCCTAAAAAAGATGACCGTGTCATCGATGCTTACTGCGGTGTTGGGACGATTGGTTTTGC
AGTTGCCAAAAAAGTCAAATCGGTTCACGGAATGGATATTACACCAGAATCAATCTTTGATGCGCGTGAAAATGCTAAAC
GTTTAGGGCTTAAAAATTGCCACTATGAAATTGGTAAAGCTGAACGTATTATTCCAAACTGGAATAAATCTGGTCACCGT
GCGACTGCCATGATTGTTGACCCACCCCGTACTGGTCTTGATGATGCCTTGCTTGAAACAATTACTAAATTCCCACCGGA
AAAAATGGTTTATGTCAGCTGTAACGTATCAACTTTAGCAAAAGATTTAGTCGTTTTGGCCAATTATTACAAAGTAGAAT
ACATTCAATCAGTGGATATGTTCCCACATACAGCGCGGACTGAAGCAGTTGTTAAATTGACAAAACGCGATGAACCTTTG
AAAATTGTTAAATTTGAACATTTGGAAGAAGAACGTAAAGCTGAAGCATTTGCTAAAGGAAAAAAACGTGGACGTCGTCC
GCAAAAATATGGTAAATACTGA

Upstream 100 bases:

>100_bases
AATGTTATAATAGTGTATCACACAATCCTTTATTGCTGATTTTTTATAATGGATTGAAAATGGTCATTAATTGACGAACT
AGAGGGAAAATAATTAACGA

Downstream 100 bases:

>100_bases
GAAATTTACTGACAGAATGGCTATTTGGCTATAAATTCATTTATTTGAGGAGAAATTTTCGTCAGTAATTTTTAGGAAAA
ATGAGAGAAAAAATGAAAAA

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKIQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEASKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPKKTSEIYGERTQVLFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANYYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY

Sequences:

>Translated_513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKIQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEASKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPKKTSEIYGERTQVLFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANYYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
>Mature_513_residues
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLVKIIEKSPNRVKPQDRAYHEM
SQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRATLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVN
LDNCLVQDPKIQEIINHICRLIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEASKRQ
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPKKTSEIYGERTQVLFNEKETITEGVLDYEFELSPRAFYQLNSEQANVLYG
EAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDITPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHR
ATAMIVDPPRTGLDDALLETITKFPPEKMVYVSCNVSTLAKDLVVLANYYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI269784680, Length=530, Percent_Identity=25.0943396226415, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI269784676, Length=530, Percent_Identity=25.0943396226415, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI20149688, Length=530, Percent_Identity=25.0943396226415, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI269784678, Length=376, Percent_Identity=25.7978723404255, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1787083, Length=349, Percent_Identity=25.214899713467, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789148, Length=476, Percent_Identity=22.6890756302521, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790403, Length=155, Percent_Identity=28.3870967741935, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=26.0606060606061, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322909, Length=442, Percent_Identity=25.1131221719457, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24666579, Length=450, Percent_Identity=30.2222222222222, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 58880; Mature: 58880

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLV
CEECCCCCEEEEEEEEECCCCCCEEEEECEEEEEEECCCCCCEEEEEHHCCCCHHHHHHH
KIIEKSPNRVKPQDRAYHEMSQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRA
HHHHCCCCCCCCHHHHHHHHHHHCEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVNLDNCLVQDPKIQEIINHICR
EECCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHH
LIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEASKRQ
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHH
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPKKTSEIYGERTQVLFNEKETITEGVLDYEF
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCCHHHEEECCHHHHHHCCCCEEE
ELSPRAFYQLNSEQANVLYGEAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDI
ECCCCEEEEECCCCCCEEHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCC
TPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHRATAMIVDPPRTGLDDALLET
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ITKFPPEKMVYVSCNVSTLAKDLVVLANYYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
HHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCE
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MINLKIGQKLQLEIERMGINGEGIGVISGRLVFIPYALPGEEVLVEITENARNFSRAKLV
CEECCCCCEEEEEEEEECCCCCCEEEEECEEEEEEECCCCCCEEEEEHHCCCCHHHHHHH
KIIEKSPNRVKPQDRAYHEMSQSHIMHLSYPMQLEFKRDVMRQALEKYKPAGWRNYELRA
HHHHCCCCCCCCHHHHHHHHHHHCEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TLGMENTLGYRNKLQFQVRRLNDGTVIAGLYQEGTHHLVNLDNCLVQDPKIQEIINHICR
EECCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHCCCCCHHHHHHHHHH
LIEKFDLPVYDERKIGGIRTVMVRRSQKTGEVQIIFVTSTPIILDGQVWPELREEASKRQ
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHH
KNSFVKFDKMLSALTEEFEEIVTVAVNFHPKKTSEIYGERTQVLFNEKETITEGVLDYEF
HHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCCHHHEEECCHHHHHHCCCCEEE
ELSPRAFYQLNSEQANVLYGEAVKALNPKKDDRVIDAYCGVGTIGFAVAKKVKSVHGMDI
ECCCCEEEEECCCCCCEEHHHHHHHCCCCCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCC
TPESIFDARENAKRLGLKNCHYEIGKAERIIPNWNKSGHRATAMIVDPPRTGLDDALLET
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCHHHHHHH
ITKFPPEKMVYVSCNVSTLAKDLVVLANYYKVEYIQSVDMFPHTARTEAVVKLTKRDEPL
HHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCE
KIVKFEHLEEERKAEAFAKGKKRGRRPQKYGKY
EEEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]