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The map label for this gene is rlmH [H]

Identifier: 125625176

GI number: 125625176

Start: 2360578

End: 2361057

Strand: Reverse

Name: rlmH [H]

Synonym: llmg_2418

Alternate gene names: 125625176

Gene position: 2361057-2360578 (Counterclockwise)

Preceding gene: 125625182

Following gene: 125625175

Centisome position: 93.34

GC content: 36.67

Gene sequence:

>480_bases
ATGAAAATTAAATTAGTAGTTGTTGGAAAATTGAAAGAGAAGTATCTAAAAGATGGAATTGCCGAATATGTTAAACGAAT
GGGAACGATGCTTCCACTTGAAATTATTGAGCTAGCTGATGAAAAAATTCCGGATAATGCTTCTGAGAAAGAAGCAGAAG
CTTTAAAGAAGCGAGAAGGGGATAAAATTTTATCTCGCATTCAAACAGGAGATAAGTTAGCAATTTTAGCAATTCAAGGG
AAGTTGATGTCATCAGAGGAATTGGCTGATTTTGTGAAAAAAGCAGAAGTTTATGGAACAGGCAATCTCGTTTTCGTAAT
TGGAGGGTCTTTGGGACTTTCCGAGGAGGTCTATAAAAGAAGTGATTTGCAAATTTCATTTGGTCGGATGACTCTCCCTC
ATCAGCTGATGCGTTTGGTTTTAGTGGAACAGATTTATCGAGCGCAAATGATAAATCGAGGTTCGGTTTATCATAAGTAA

Upstream 100 bases:

>100_bases
TAAATGTTTTTATTGCCTAAGGTGTAAAAGTGAGTAAAAAATTGGGGAAAAAGTTTTTAATTTTTAAGTAATAAAAGCTT
TGATTGGGAGAAAAATAAAG

Downstream 100 bases:

>100_bases
AAATGAAAGAATGAGATATGATTAATATAATAGAAAAATTCCGCAAAAATAAAAATTTAGAAAATGCGGAAAAACAAGCG
GCTTATTTACGCCATCAGTT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGDKILSRIQTGDKLAILAIQG
KLMSSEELADFVKKAEVYGTGNLVFVIGGSLGLSEEVYKRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSVYHK

Sequences:

>Translated_159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGDKILSRIQTGDKLAILAIQG
KLMSSEELADFVKKAEVYGTGNLVFVIGGSLGLSEEVYKRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSVYHK
>Mature_159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGDKILSRIQTGDKLAILAIQG
KLMSSEELADFVKKAEVYGTGNLVFVIGGSLGLSEEVYKRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSVYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17897; Mature: 17897

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREG
CEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCH
DKILSRIQTGDKLAILAIQGKLMSSEELADFVKKAEVYGTGNLVFVIGGSLGLSEEVYKR
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
SDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSVYHK
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREG
CEEEEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCH
DKILSRIQTGDKLAILAIQGKLMSSEELADFVKKAEVYGTGNLVFVIGGSLGLSEEVYKR
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
SDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSVYHK
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]