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The map label for this gene is recF

Identifier: 125624996

GI number: 125624996

Start: 2191274

End: 2192353

Strand: Direct

Name: recF

Synonym: llmg_2229

Alternate gene names: 125624996

Gene position: 2191274-2192353 (Clockwise)

Preceding gene: 125624995

Following gene: 125624998

Centisome position: 86.63

GC content: 35.46

Gene sequence:

>1080_bases
ATGAAACTCAAGCAAATTGAACTTAAAAATTTTCGGAATTATGAAGATTTAAAACTCGACTTCCATCCCAATCTCAATAT
CTTCTTGGGTCAAAATGCTCAAGGAAAGACAAATATTCTTGAAGCTATTCACTTTTTAGCCCTTACTCGTAGTCACAGAA
CGAGTCATGACAAAGAGTTAATTCGTTGGTCTGGGCAAGAAATGAAAGTTTCAGGATTGGTTGAAAAGGCTCATGCAACC
GTTCCTCTTGAAGTTCAATTAAGTTCCAAAGGACGAATTGCTAAGGCTAATCATCTGAAGGAAAATCGCTTGGCTGACTA
TATCGGACAACTAAAAATCTTGATGTTTGCGCCTGAAAATTTAGAGTTGGTCAAAGGTTCGCCGGCCACTCGCAGACGAT
TTATGGATATTGAACTCGGACAAATTCACGCTGTTTATCTTTATGATTCCATGCGTTATAATCGTGCTTTAAAAGAGCGA
AATGCTTATTTGAAATTTGACCAAGCAAAGATTGACAAAAACTTTTTGACGGTTCTTGATGAACAATTGGCTGAACACGG
CAATAAAATCATGTTTGAACGTAAAACTTTCATTGAAAAGCTTGAAATTCATGCAAAAAAAATTCATGAACAGTTAACTC
ATGGTTTGGAAACATTAAAAATCACTTATAATCAAAATGTAAAAACTGATTTTTCAAAAGAATTATTGAGTCGACAAGAT
CATGATATTTTTAGACATCAGACTACCGTCGGCCCCCATCGAGATGATTTACAGTTCTTTATCAATGATATCAATGTTGC
TGATTTTGGTAGTCAGGGACAACAACGAACCGTTGCTCTTTCGATTAAATTGGCCGAAATTGATTTGATTTTTGAGGAAA
CGGGTGAGTATCCTATTTTACTTTTAGACGATGTCATGAGTGAACTTGACAATCATCGTCAGCTGGATTTGATTGAAACA
AGTTTAGGTAAAACCCAGACTTTTATTACCACAACCACTCTTGACCATCTCAAAAATTTGCCGGAAAATTTGAGTATTTT
CCATGTTACTGATGGGACAATTGAGAAAGAAAAAGAATAA

Upstream 100 bases:

>100_bases
AGGAAGCTCGTGTCAAAGCCATTGTTAAAAAAATGAATGCAGAAAACAAAAGCACGAAGCCTACTAAAAAAACACCCCCT
CATTTTCCTGGTAGAAAATA

Downstream 100 bases:

>100_bases
AAAATTACTGATGAAACCACTATCATATTCGTAAAAGCTTAAGCGTCAACGACTATCCTATGATGGAAATTTTGTCAGTA
ATTTTTTGTATCTGTCAGTA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKLKQIELKNFRNYEDLKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELIRWSGQEMKVSGLVEKAHAT
VPLEVQLSSKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRRFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDQAKIDKNFLTVLDEQLAEHGNKIMFERKTFIEKLEIHAKKIHEQLTHGLETLKITYNQNVKTDFSKELLSRQD
HDIFRHQTTVGPHRDDLQFFINDINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVTDGTIEKEKE

Sequences:

>Translated_359_residues
MKLKQIELKNFRNYEDLKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELIRWSGQEMKVSGLVEKAHAT
VPLEVQLSSKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRRFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDQAKIDKNFLTVLDEQLAEHGNKIMFERKTFIEKLEIHAKKIHEQLTHGLETLKITYNQNVKTDFSKELLSRQD
HDIFRHQTTVGPHRDDLQFFINDINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVTDGTIEKEKE
>Mature_359_residues
MKLKQIELKNFRNYEDLKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELIRWSGQEMKVSGLVEKAHAT
VPLEVQLSSKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRRFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDQAKIDKNFLTVLDEQLAEHGNKIMFERKTFIEKLEIHAKKIHEQLTHGLETLKITYNQNVKTDFSKELLSRQD
HDIFRHQTTVGPHRDDLQFFINDINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVTDGTIEKEKE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=374, Percent_Identity=24.8663101604278, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LACLM (A2RNA8)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001033479.1
- STRING:   A2RNA8
- GeneID:   4797336
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_2229
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41641; Mature: 41641

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKQIELKNFRNYEDLKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKEL
CCCCEEHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IRWSGQEMKVSGLVEKAHATVPLEVQLSSKGRIAKANHLKENRLADYIGQLKILMFAPEN
HHHCCCEEEHHHHHHHHCCCCEEEEEECCCCCEEHHHCCHHHHHHHHHCCEEEEEECCCC
LELVKGSPATRRRFMDIELGQIHAVYLYDSMRYNRALKERNAYLKFDQAKIDKNFLTVLD
CEEEECCCHHHHEEEEEEECCEEEEEEECCHHHHHHHHHCCCEEEEHHHHHCHHHHHHHH
EQLAEHGNKIMFERKTFIEKLEIHAKKIHEQLTHGLETLKITYNQNVKTDFSKELLSRQD
HHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCC
HDIFRHQTTVGPHRDDLQFFINDINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPIL
CHHHHHCCCCCCCCCCHHHEEECCCEECCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEE
LLDDVMSELDNHRQLDLIETSLGKTQTFITTTTLDHLKNLPENLSIFHVTDGTIEKEKE
EHHHHHHHHCCCCEEEHHHHCCCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKLKQIELKNFRNYEDLKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKEL
CCCCEEHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IRWSGQEMKVSGLVEKAHATVPLEVQLSSKGRIAKANHLKENRLADYIGQLKILMFAPEN
HHHCCCEEEHHHHHHHHCCCCEEEEEECCCCCEEHHHCCHHHHHHHHHCCEEEEEECCCC
LELVKGSPATRRRFMDIELGQIHAVYLYDSMRYNRALKERNAYLKFDQAKIDKNFLTVLD
CEEEECCCHHHHEEEEEEECCEEEEEEECCHHHHHHHHHCCCEEEEHHHHHCHHHHHHHH
EQLAEHGNKIMFERKTFIEKLEIHAKKIHEQLTHGLETLKITYNQNVKTDFSKELLSRQD
HHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCC
HDIFRHQTTVGPHRDDLQFFINDINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPIL
CHHHHHCCCCCCCCCCHHHEEECCCEECCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEE
LLDDVMSELDNHRQLDLIETSLGKTQTFITTTTLDHLKNLPENLSIFHVTDGTIEKEKE
EHHHHHHHHCCCCEEEHHHHCCCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA