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The map label for this gene is metK [H]

Identifier: 125624931

GI number: 125624931

Start: 2119789

End: 2120988

Strand: Reverse

Name: metK [H]

Synonym: llmg_2160

Alternate gene names: 125624931

Gene position: 2120988-2119789 (Counterclockwise)

Preceding gene: 125624932

Following gene: 125624930

Centisome position: 83.85

GC content: 41.08

Gene sequence:

>1200_bases
ATGTCAGAAAAACATTTATTTACATCAGAATCAGTATCAGAAGGGCATCCAGATAAGGTTGCTGACCAAATCTCAGATGC
CATTCTTGATGCCATTCTCGCACAAGACCCACATGCACACGTTGCTTGTGAAACGGTCGTATATACAGGGACAGTCAATG
TCTTTGGAGAAATATCAACATCAGCTTACGTTGATATTGCCCATGTCGTTCGCGAAACCATCAAAAAAATTGGCTATACT
GATAGCGAAAATGGTTTTGATTACAAATCAGTCGGTGTTCACGTTTCACTCGTTGAGCAATCAAGCGATATTGCTCAAGG
GGTAAACGAAGCTGAAGAAGTTCGTGATAAAAATGGTCAGTTAGTTGACCCACTTGATTTGATTGGTGCTGGTGACCAAG
GAATGATGTTCGGTTTTGCAACTAACGAAACTGCCGAATACATGCCACTTGCCATTTCACTTTCACATAAATTAGTTAAG
AAATTGGCTGATTTGCGTAAATCAGGTGAAATTTCATACTTGCGTCCAGATGCAAAATCACAAGTGACTGTAGAATATGA
TAATAATGGAAAAGCAAAACGTGTTGATACCGTTGTTATCTCGACTCAACATGCTGCTACGGCAACAAATGAAGAAATTC
ATGATGATGTGATTAACAAAGTGATTAAGGCTATTATTCCTGCCGAACTTTTGGATGATGAAACAAAATACTTTATCAAT
CCAACAGGACGTTTTGTTATCGGTGGTCCTCAAGGAGACTCAGGTTTGACTGGCCGTAAGATTATTGTTGATACTTACGG
TGGTTATGCACCACATGGTGGTGGGGCCTTCTCAGGTAAGGATGCAACTAAGGTTGACCGTTCAGCTTCTTACGCGGCTC
GTTATGTCGCAAAAAACATTGTGGCTGCTGGACTTGCTGATAAAGCTCAAATTCAACTTTCTTATGCGATTGGTGTGGCC
ACTCCTACATCAATCAATGTTGAGACTTTTGGTACAGGTAAAGTTTCAGATGATGAACTTTTGGCCGCGATCCGCAAGGT
CTTTGACCTCCGTCCAGCAGGAATTATCCAAATGCTTGACTTGCTTCGTCCAATTTATGGACAAACAGCAGCTTATGGGC
ACTTTGGCCGTACGGATATTGAATTGCCATGGGAACAAACAGACAAGGTTGAAGAATTGAAAGCTGTTTTAGGTAAATAA

Upstream 100 bases:

>100_bases
GAAGCAATAACAGCTATCCTATGACAGAACTTTCTGTCAGTAAAATTGAATAAGTTTAAAGACGCCTTGTAACCAAAATT
TAGATTGGGGGACATTTTTA

Downstream 100 bases:

>100_bases
AATGCCTCGCCGAACTATGTTCGGTCTGACCTGACTTTGTCAGCTCCGCTGTCCTTTTTATCTATCCGCTAAAGCGAATG
ACAAAAAGGCACTTCGGCCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYT
DSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVK
KLADLRKSGEISYLRPDAKSQVTVEYDNNGKAKRVDTVVISTQHAATATNEEIHDDVINKVIKAIIPAELLDDETKYFIN
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLADKAQIQLSYAIGVA
TPTSINVETFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDIELPWEQTDKVEELKAVLGK

Sequences:

>Translated_399_residues
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYT
DSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVK
KLADLRKSGEISYLRPDAKSQVTVEYDNNGKAKRVDTVVISTQHAATATNEEIHDDVINKVIKAIIPAELLDDETKYFIN
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLADKAQIQLSYAIGVA
TPTSINVETFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDIELPWEQTDKVEELKAVLGK
>Mature_398_residues
SEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEISTSAYVDIAHVVRETIKKIGYTD
SENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQLVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKK
LADLRKSGEISYLRPDAKSQVTVEYDNNGKAKRVDTVVISTQHAATATNEEIHDDVINKVIKAIIPAELLDDETKYFINP
TGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNIVAAGLADKAQIQLSYAIGVAT
PTSINVETFGTGKVSDDELLAAIRKVFDLRPAGIIQMLDLLRPIYGQTAAYGHFGRTDIELPWEQTDKVEELKAVLGK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=57.7319587628866, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=57.5835475578406, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=62.5316455696203, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=59.3264248704663, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=407, Percent_Identity=57.985257985258, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=57.8005115089514, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=402, Percent_Identity=54.9751243781095, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=407, Percent_Identity=52.8255528255528, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=56.2130177514793, Blast_Score=353, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=54.5189504373178, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=329, Percent_Identity=55.0151975683891, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=66.6666666666667, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=85, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=57.840616966581, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=56.7430025445293, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=56.0102301790281, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=55.2699228791774, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=55.2699228791774, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580541, Length=391, Percent_Identity=52.9411764705882, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=54.6875, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42974; Mature: 42843

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCC
SAYVDIAHVVRETIKKIGYTDSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECCCHHHHHCCCHHHHHHHCCCC
LVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKKLADLRKSGEISYLRPDAKS
EECCHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCCCC
QVTVEYDNNGKAKRVDTVVISTQHAATATNEEIHDDVINKVIKAIIPAELLDDETKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEC
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLADKAQIQLSYAIGVATPTSINVETFGTGKVSDDELLAAIRKVFDLRPAGIIQMLD
HHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LLRPIYGQTAAYGHFGRTDIELPWEQTDKVEELKAVLGK
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SEKHLFTSESVSEGHPDKVADQISDAILDAILAQDPHAHVACETVVYTGTVNVFGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEECCC
SAYVDIAHVVRETIKKIGYTDSENGFDYKSVGVHVSLVEQSSDIAQGVNEAEEVRDKNGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECCCHHHHHCCCHHHHHHHCCCC
LVDPLDLIGAGDQGMMFGFATNETAEYMPLAISLSHKLVKKLADLRKSGEISYLRPDAKS
EECCHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCCCC
QVTVEYDNNGKAKRVDTVVISTQHAATATNEEIHDDVINKVIKAIIPAELLDDETKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEEC
PTGRFVIGGPQGDSGLTGRKIIVDTYGGYAPHGGGAFSGKDATKVDRSASYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLADKAQIQLSYAIGVATPTSINVETFGTGKVSDDELLAAIRKVFDLRPAGIIQMLD
HHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LLRPIYGQTAAYGHFGRTDIELPWEQTDKVEELKAVLGK
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]