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The map label for this gene is priA [H]

Identifier: 125624924

GI number: 125624924

Start: 2113093

End: 2115438

Strand: Reverse

Name: priA [H]

Synonym: llmg_2153

Alternate gene names: 125624924

Gene position: 2115438-2113093 (Counterclockwise)

Preceding gene: 125624925

Following gene: 125624923

Centisome position: 83.63

GC content: 37.98

Gene sequence:

>2346_bases
ATGAAAATTGCTAAAGTAATCGTTGACCTGCCTCTGATGCAGACTGATAAGCCTTTTTCTTATGCCATTTCAAAAGAATT
TCAAAGTTTGATTGAACTGGGAATGCGGGTTCATGTTCCTTTTGGGACAGGAAATCGTTTGGTTCAAGGAATTATTGTTG
GGATTGAGGAAGAAAAGGATGCAGAAAATTCGTCAGTAAATTTGAAAGATATTGCTGAGTTACTGGATGTTGAACCGGTG
CTGACCGAAGAACAACTATGGTTAGCTGATGAAATGCGCCACTCGGTTTTCTCATATAAAATTTCTTGTTTGAAAGCGAT
GCTGCCTAATCTTTTGAATTCTAATTATGACAAACTCTTGAAATCTGCTGACGGAGCTGTCAGCAAATGGTCACAGCTGA
CAGAAGAAGAAAAAATTTCGTCAGCAAAAGCGGTGCGTGCTGGACAGCTGTCAGTAGAATATTTAGCTAAATCCAAAGAA
AATCGGAGGACCGAAAAATATTTATCTATTCCGGATTTGGAAAAATTGGCTGAATTTGAAATTGAAAAACGAGCGAAAAA
ACGCTTGGAGCTAAAATATTTTCTCTTACGAATGACCGAAGAAAAAATTGCTCTAAAATCTTTAAATTTTTCTTCACAAA
TTATCAAATTTTTCATTGAGCATGATTTGGTAAAAATTGAGGAAGTTGAAATTTCTCGGACAAAAGATAAATTTGAAGCG
ATTGAAAATGATAGCGCCAAAGTGCTGAATTCTGAACAAGAATCAGCTTATGAAAAAATAATTGCTTCTTCTGACAAACC
ATTTTTGTTGGAAGGCATCACTGGCTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTACAAATGGGAA
AAACGGCCATCATGCTTGTGCCGGAAATTTCACTGACGCCACAGATTACCAATCGATTTATTGCTCGTTTTGGTGACAAA
GTGGCCATCATGCACTCAGGTTTGTCTGACGGTGAAAAATATGATGAATGGCGTAAAATTAAGTCTGGCAAAGCTCAAGT
CGTTGTCGGTGCTCGCTCGGCAATTTTTACGCCAGTCGAAAATCTGGGTATTATCATCATTGATGAGGAGCACGAAACTT
CCTATAAGCAAGATTCTAGTCCGCGTTATCATGCCAGAGATATTGCACTTTTGCGAGCAAAATGGCATGGAGCTACCCTT
GTTTTGGGTTCAGCAACGCCAAGTTTAGAATCACGGGCGCGTGCCATGCGTGGCGTTTATGAGCTTTTATTACTGACAAA
GCGGGCAAATGAAGCAGCAATCTTGCCAGAAATTAAAATCATTGATATGCGCAGTGAAATTTCTGACCAGTCAGCAAATT
TCTCACAATCATTACTGGCAAAAATTAAAGAAAAAATTGACAAAGACGAACAAGTCATTTTGATGCTGAATCGTCGAGGT
TATTCTTCATTTGTCATGTGTCGAGAATGTGGTTTTGTTGTTGATTGCCCCAACTGTGACATTTCATTAACCCTTCACAT
GGACACCAGAACTCTGAATTGTCATTATTGTGGTCACGTTCAGGGCATTCCCCAAACTTGCCCCAACTGTCAATCGCGAA
AAATTCGTTATTATGGTTCTGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAGGCTCGAGTTTTACGA
ATGGATGTGGATACAACTCGAACGAAAAATGCTCACGAAAAAATTCTTGAAAAATTTGGTAACCATGAAGCTGATATCCT
TTTAGGAACTCAGATGATTGCCAAAGGATTAGATTTTCCAAACGTGACTTTAGTTGGTGTCATTAATGCAGATACCGGTC
TAAATTTACCTGATTTTCGGGCCAGTGAACGCTCGTTTGAATTGCTGATGCAGGTGGCTGGACGAGCTGGACGTGCGGAT
AAAAAAGGTGAAGTTTTGATTCAAACCTTTAATCCACATCATTATGCCATTGAAATGACCAAAGCTCAAGATTATGAAAA
ATTTTATGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACGGTTCAAGTGCTTGTTTCTC
ATCAAGATGAAGAAGTTGCCATTCAAAAATCCTATGAAATTTCCCAAATTTTAAAGCAAAATCTGACAGAAAAAGCCAAA
ATTTTGGGACCAACTGCCAGACCAATTGCCAGAACACATAATCTTTATCATTATCAAATTTTAGTTAAATATCGTTTTGA
AGAAGAATTAGAGTCCGCTTTGAATCAAGTGCTTGAATTAACCCAACTTCGTGACAATAAAAATTTACGAATCTTGATTG
ATAGCGAACCACAAAATTTTGTATAA

Upstream 100 bases:

>100_bases
GACAGAATCAAAGTTTATTCCATTGTCTAGTCGTAATTGGCAAATATCAAATAGTGAACTGTGTTATAATTAAAAGAAGA
ATTACGGAGGTCAAAACTGA

Downstream 100 bases:

>100_bases
AATCAGAGTTGATGAGGTTAAAAATGATTAGATTAACCAAAGAAGAAGCCAGAAATTTTTTATTGGCTAAGCAGGGTTTG
CTTGGTCATAAACAGTTTTC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 781; Mature: 781

Protein sequence:

>781_residues
MKIAKVIVDLPLMQTDKPFSYAISKEFQSLIELGMRVHVPFGTGNRLVQGIIVGIEEEKDAENSSVNLKDIAELLDVEPV
LTEEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSADGAVSKWSQLTEEEKISSAKAVRAGQLSVEYLAKSKE
NRRTEKYLSIPDLEKLAEFEIEKRAKKRLELKYFLLRMTEEKIALKSLNFSSQIIKFFIEHDLVKIEEVEISRTKDKFEA
IENDSAKVLNSEQESAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLQMGKTAIMLVPEISLTPQITNRFIARFGDK
VAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFTPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGATL
VLGSATPSLESRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLAKIKEKIDKDEQVILMLNRRG
YSSFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVQGIPQTCPNCQSRKIRYYGSGTQKVEEELREIMPEARVLR
MDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRAD
KKGEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEISQILKQNLTEKAK
ILGPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRILIDSEPQNFV

Sequences:

>Translated_781_residues
MKIAKVIVDLPLMQTDKPFSYAISKEFQSLIELGMRVHVPFGTGNRLVQGIIVGIEEEKDAENSSVNLKDIAELLDVEPV
LTEEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSADGAVSKWSQLTEEEKISSAKAVRAGQLSVEYLAKSKE
NRRTEKYLSIPDLEKLAEFEIEKRAKKRLELKYFLLRMTEEKIALKSLNFSSQIIKFFIEHDLVKIEEVEISRTKDKFEA
IENDSAKVLNSEQESAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLQMGKTAIMLVPEISLTPQITNRFIARFGDK
VAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFTPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGATL
VLGSATPSLESRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLAKIKEKIDKDEQVILMLNRRG
YSSFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVQGIPQTCPNCQSRKIRYYGSGTQKVEEELREIMPEARVLR
MDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRAD
KKGEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEISQILKQNLTEKAK
ILGPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRILIDSEPQNFV
>Mature_781_residues
MKIAKVIVDLPLMQTDKPFSYAISKEFQSLIELGMRVHVPFGTGNRLVQGIIVGIEEEKDAENSSVNLKDIAELLDVEPV
LTEEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSADGAVSKWSQLTEEEKISSAKAVRAGQLSVEYLAKSKE
NRRTEKYLSIPDLEKLAEFEIEKRAKKRLELKYFLLRMTEEKIALKSLNFSSQIIKFFIEHDLVKIEEVEISRTKDKFEA
IENDSAKVLNSEQESAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLQMGKTAIMLVPEISLTPQITNRFIARFGDK
VAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFTPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGATL
VLGSATPSLESRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLAKIKEKIDKDEQVILMLNRRG
YSSFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVQGIPQTCPNCQSRKIRYYGSGTQKVEEELREIMPEARVLR
MDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRAD
KKGEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEISQILKQNLTEKAK
ILGPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRILIDSEPQNFV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=792, Percent_Identity=33.9646464646465, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 89159; Mature: 89159

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAKVIVDLPLMQTDKPFSYAISKEFQSLIELGMRVHVPFGTGNRLVQGIIVGIEEEKD
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHEEEHHEEEECCCCC
AENSSVNLKDIAELLDVEPVLTEEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KSADGAVSKWSQLTEEEKISSAKAVRAGQLSVEYLAKSKENRRTEKYLSIPDLEKLAEFE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHHHCCCCCHHHHHHHH
IEKRAKKRLELKYFLLRMTEEKIALKSLNFSSQIIKFFIEHDLVKIEEVEISRTKDKFEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHH
IENDSAKVLNSEQESAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLQMGKTAIMLV
HCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEE
PEISLTPQITNRFIARFGDKVAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFTPVE
ECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHCCHH
NLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGATLVLGSATPSLESRARAMRGVY
HCCEEEEECCCCCCCCCCCCCCEEHHHEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
ELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLAKIKEKIDKDEQVILMLNRRG
HHHHHHHCCCCCEECCCEEEEECHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
YSSFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVQGIPQTCPNCQSRKIRYYGS
CCHHHHHHHCCCEEECCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEECC
GTQKVEEELREIMPEARVLRMDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFP
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCC
NVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKKGEVLIQTFNPHHYAIEMT
CEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEEC
KAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEISQILKQNLTEKAK
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHH
ILGPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRILIDSEPQNF
HCCCCCCHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
V
C
>Mature Secondary Structure
MKIAKVIVDLPLMQTDKPFSYAISKEFQSLIELGMRVHVPFGTGNRLVQGIIVGIEEEKD
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHEEEHHEEEECCCCC
AENSSVNLKDIAELLDVEPVLTEEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KSADGAVSKWSQLTEEEKISSAKAVRAGQLSVEYLAKSKENRRTEKYLSIPDLEKLAEFE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHHHCCCCCHHHHHHHH
IEKRAKKRLELKYFLLRMTEEKIALKSLNFSSQIIKFFIEHDLVKIEEVEISRTKDKFEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHH
IENDSAKVLNSEQESAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLQMGKTAIMLV
HCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHCCCEEEEEE
PEISLTPQITNRFIARFGDKVAIMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFTPVE
ECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHCCHH
NLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGATLVLGSATPSLESRARAMRGVY
HCCEEEEECCCCCCCCCCCCCCEEHHHEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
ELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLAKIKEKIDKDEQVILMLNRRG
HHHHHHHCCCCCEECCCEEEEECHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
YSSFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVQGIPQTCPNCQSRKIRYYGS
CCHHHHHHHCCCEEECCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEECC
GTQKVEEELREIMPEARVLRMDVDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFP
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCC
NVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKKGEVLIQTFNPHHYAIEMT
CEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEEEC
KAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEISQILKQNLTEKAK
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHH
ILGPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRILIDSEPQNF
HCCCCCCHHHHHCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]