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The map label for this gene is ps428 [H]

Identifier: 125624885

GI number: 125624885

Start: 2094491

End: 2094910

Strand: Reverse

Name: ps428 [H]

Synonym: llmg_2114

Alternate gene names: 125624885

Gene position: 2094910-2094491 (Counterclockwise)

Preceding gene: 125624886

Following gene: 125624884

Centisome position: 82.82

GC content: 38.33

Gene sequence:

>420_bases
ATGACAAGAGGATTTAAAAAACTAGACGGAAATGCGACTATTCCAGAACGAGCGACAGAACATAGCGCAGGGTATGACAT
TTCATCAAGTGAAACAGTTACGATTCAACCTGATGAAATTAAAATGGTAAGCACTGGGCTAGCTGTTCAACTTGGTGATG
ATGAAGTATTGAAATTATACGACCGTTCAAGTAATCCAGTTAAGCGTGGCATTGCATTGATTAATTCAGTAGGGATTATC
GATTCAGATTACTATCCGCAAGAATTTAAAGGCTTATTTATGAACATCTCAAAAGAGCCTGTAACCATTTCTAAAGGTCA
AAGAATAATGCAAGGGGTATTTGTCAAACACCTTACAATAGACGATGACAACGCAAATGGAAAGCGTACAGGCGGTTTTG
GAAGTACTGGGGAGAAATAA

Upstream 100 bases:

>100_bases
GTTCTACCATAAATTTAACTAATGATTTCAAATTCACCCAGCAAGAAATCGACAGCATGCAAACTGGGAGCTATGAGCTT
GTGCCTGTGGAGGACGGAGA

Downstream 100 bases:

>100_bases
GTGACAACAACAGATAAAATCGTGCAAGACTACCAAATAAAGTTGTTAAAGATTATATTCAAAGAAATTGATAATCTGAT
GACGAAAAAAGAAAATGCGG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MTRGFKKLDGNATIPERATEHSAGYDISSSETVTIQPDEIKMVSTGLAVQLGDDEVLKLYDRSSNPVKRGIALINSVGII
DSDYYPQEFKGLFMNISKEPVTISKGQRIMQGVFVKHLTIDDDNANGKRTGGFGSTGEK

Sequences:

>Translated_139_residues
MTRGFKKLDGNATIPERATEHSAGYDISSSETVTIQPDEIKMVSTGLAVQLGDDEVLKLYDRSSNPVKRGIALINSVGII
DSDYYPQEFKGLFMNISKEPVTISKGQRIMQGVFVKHLTIDDDNANGKRTGGFGSTGEK
>Mature_138_residues
TRGFKKLDGNATIPERATEHSAGYDISSSETVTIQPDEIKMVSTGLAVQLGDDEVLKLYDRSSNPVKRGIALINSVGIID
SDYYPQEFKGLFMNISKEPVTISKGQRIMQGVFVKHLTIDDDNANGKRTGGFGSTGEK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Escherichia coli, GI1790071, Length=134, Percent_Identity=34.3283582089552, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15156; Mature: 15025

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGFKKLDGNATIPERATEHSAGYDISSSETVTIQPDEIKMVSTGLAVQLGDDEVLKLY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCEEEEECCCCEEEEE
DRSSNPVKRGIALINSVGIIDSDYYPQEFKGLFMNISKEPVTISKGQRIMQGVFVKHLTI
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCCCEEECCCHHHHHHHHEEEEEE
DDDNANGKRTGGFGSTGEK
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRGFKKLDGNATIPERATEHSAGYDISSSETVTIQPDEIKMVSTGLAVQLGDDEVLKLY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCEEEEECCCCEEEEE
DRSSNPVKRGIALINSVGIIDSDYYPQEFKGLFMNISKEPVTISKGQRIMQGVFVKHLTI
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCCCEEECCCHHHHHHHHEEEEEE
DDDNANGKRTGGFGSTGEK
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]