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The map label for this gene is murE [H]

Identifier: 125624766

GI number: 125624766

Start: 1976229

End: 1977680

Strand: Direct

Name: murE [H]

Synonym: llmg_1989

Alternate gene names: 125624766

Gene position: 1976229-1977680 (Clockwise)

Preceding gene: 125624763

Following gene: 125624769

Centisome position: 78.13

GC content: 36.78

Gene sequence:

>1452_bases
ATGATTAAACTCGAACAAGTTGTAGAAATACTAAAAAAAGATAATAACTTTCGTGAGATTTCTTCTGCTGGCGAATATTA
TTTCAATTGGCCAAAAGAAGTAAACTTTGACCAGCTCTCTTATGATTCACGAAAATCAACTAAAGACACCCTTTTTTTTG
CCAAGGGTTTAAATTTTAAAAAGGAGTATCTGACAGATTTAGAAGCTGCTTTTTATGTTTCAGAATTTGATTATGAAGTA
GCTCTTCCAGCAATTATCGTAACCGATGTTAAAAGAGCAATGGCTCTAATTGCCGCTAACTTTTATGAATTCCCTCAAAA
TAAATTAAAAACCCTTGCCTTAACTGGGACCAAAGGAAAAACAACTTCTGCTTACTTTGCTAAAGCCATTCTTGATAAAA
TGAATGGAGGTAAAACTGCCCTTTTATCAACTGCCCAAACGACTCTTGATGGACAAAATTATTTCAAATCAGAACTGACA
ACACCTGAAAGTCTTGATTTATTGGAAATGATGGCAAAAGCTCTGGAAAATGGAATGACACATTTAGTAATGGAAGTTTC
AAGCCAAGCTTACAAGACTGAACGAGTTTATGGTTTAACTTTTGATGTTGGTGTTTTCTTAAATATTTCTCCTGACCACA
TCGGTCCAGTTGAGCACCCAACTCTTGAAGATTATTTTTACTGCAAACGTCAACTTCTTAAAAATTCACGTTATTTTGTT
GCCAATGCCGAAATGAACCACTTTGCAATCATTAAAGAAGAACTCAATGAACGGAAAATCCCTCATGCTTTCTATGGTGC
AGATTCTGAAAATAAAATTATAGAATCAAAAGGACTTCATTTTGTTACTGACGGCTCTGTCAGTGGAGAATTTGATATTC
GCTTGTTAGGACGATTCAACCAAGAAAACGCCTTGGCTACAGCATTAGCAACCAAAGCACTTGGTGCTTCTTTTGAAAAT
ATTCGTCAAGGTTTAGCTTCAGCTATCGTTCCAGGAAGAATGGAATTACTCACCGCCAAAAATGGGGCACACATTTACAT
TGACTATGCCCACAATGGTTTATCGTTAGAAAATCTCGTTGAAGTTGTTGAAGACCATCATGCTGGTCAGCTTTTCTTAG
TTCTTGGTTCAACCGGAAATAAAGGAGAAAGTCGCCGTAAAGATTTCGGACAAGTCATTGAAAATCATCCCCGTCTAAAT
GTAATCTTAACAACGGACGATTCAAACCGAGAAAATCCCAAAACAATTGCTGACGAGATTGCTTCATTTGTTAGTCGTGA
GCTGGACTTTGAATTGGACCGTGAATTTGCAATTAAAAAAGCTATCAGTAAAACTCAAAATTCTGATGATGCTGTCATTA
TCGCTGGAAAAGGCGCGGATATGTTCCAATTGAAGGACGGGAAACGTGAACCTTATATCGGAGATAGTCCTGCTGCTCAA
AAATATCTCTAA

Upstream 100 bases:

>100_bases
GTCTTTGCTTATGCTATAATATAGGGTAATGGGATTTTTCCCTTAGTTCCTTGCTTTTAGAAAGGTATCGCACGCTTCGC
GATAAATAGGCATTCCTCAC

Downstream 100 bases:

>100_bases
TTTACTGACAGAAGTTCTATCAGTAAATTAACTAGAGAAAAACGACTCGAAATTGAGTCGTTTTTCTCTTTTATCCTTTA
ACTAAGCAAAAGAATTAACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MIKLEQVVEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTKDTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAANFYEFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGQNYFKSELT
TPESLDLLEMMAKALENGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANAEMNHFAIIKEELNERKIPHAFYGADSENKIIESKGLHFVTDGSVSGEFDIRLLGRFNQENALATALATKALGASFEN
IRQGLASAIVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEDHHAGQLFLVLGSTGNKGESRRKDFGQVIENHPRLN
VILTTDDSNRENPKTIADEIASFVSRELDFELDREFAIKKAISKTQNSDDAVIIAGKGADMFQLKDGKREPYIGDSPAAQ
KYL

Sequences:

>Translated_483_residues
MIKLEQVVEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTKDTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAANFYEFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGQNYFKSELT
TPESLDLLEMMAKALENGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANAEMNHFAIIKEELNERKIPHAFYGADSENKIIESKGLHFVTDGSVSGEFDIRLLGRFNQENALATALATKALGASFEN
IRQGLASAIVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEDHHAGQLFLVLGSTGNKGESRRKDFGQVIENHPRLN
VILTTDDSNRENPKTIADEIASFVSRELDFELDREFAIKKAISKTQNSDDAVIIAGKGADMFQLKDGKREPYIGDSPAAQ
KYL
>Mature_483_residues
MIKLEQVVEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTKDTLFFAKGLNFKKEYLTDLEAAFYVSEFDYEV
ALPAIIVTDVKRAMALIAANFYEFPQNKLKTLALTGTKGKTTSAYFAKAILDKMNGGKTALLSTAQTTLDGQNYFKSELT
TPESLDLLEMMAKALENGMTHLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
ANAEMNHFAIIKEELNERKIPHAFYGADSENKIIESKGLHFVTDGSVSGEFDIRLLGRFNQENALATALATKALGASFEN
IRQGLASAIVPGRMELLTAKNGAHIYIDYAHNGLSLENLVEVVEDHHAGQLFLVLGSTGNKGESRRKDFGQVIENHPRLN
VILTTDDSNRENPKTIADEIASFVSRELDFELDREFAIKKAISKTQNSDDAVIIAGKGADMFQLKDGKREPYIGDSPAAQ
KYL

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=498, Percent_Identity=25.3012048192771, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 54114; Mature: 54114

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLEQVVEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTKDTLFFAKGLNFK
CCCHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCH
KEYLTDLEAAFYVSEFDYEVALPAIIVTDVKRAMALIAANFYEFPQNKLKTLALTGTKGK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TTSAYFAKAILDKMNGGKTALLSTAQTTLDGQNYFKSELTTPESLDLLEMMAKALENGMT
CHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
HLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
HHHHHHHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ANAEMNHFAIIKEELNERKIPHAFYGADSENKIIESKGLHFVTDGSVSGEFDIRLLGRFN
EECCCCCHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCEEEECCCCCCEEEEEEEECCC
QENALATALATKALGASFENIRQGLASAIVPGRMELLTAKNGAHIYIDYAHNGLSLENLV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCHHHHH
EVVEDHHAGQLFLVLGSTGNKGESRRKDFGQVIENHPRLNVILTTDDSNRENPKTIADEI
HHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
ASFVSRELDFELDREFAIKKAISKTQNSDDAVIIAGKGADMFQLKDGKREPYIGDSPAAQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCC
KYL
CCC
>Mature Secondary Structure
MIKLEQVVEILKKDNNFREISSAGEYYFNWPKEVNFDQLSYDSRKSTKDTLFFAKGLNFK
CCCHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCH
KEYLTDLEAAFYVSEFDYEVALPAIIVTDVKRAMALIAANFYEFPQNKLKTLALTGTKGK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
TTSAYFAKAILDKMNGGKTALLSTAQTTLDGQNYFKSELTTPESLDLLEMMAKALENGMT
CHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
HLVMEVSSQAYKTERVYGLTFDVGVFLNISPDHIGPVEHPTLEDYFYCKRQLLKNSRYFV
HHHHHHHHHHHHHCEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ANAEMNHFAIIKEELNERKIPHAFYGADSENKIIESKGLHFVTDGSVSGEFDIRLLGRFN
EECCCCCHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCEEEECCCCCCEEEEEEEECCC
QENALATALATKALGASFENIRQGLASAIVPGRMELLTAKNGAHIYIDYAHNGLSLENLV
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCHHHHH
EVVEDHHAGQLFLVLGSTGNKGESRRKDFGQVIENHPRLNVILTTDDSNRENPKTIADEI
HHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
ASFVSRELDFELDREFAIKKAISKTQNSDDAVIIAGKGADMFQLKDGKREPYIGDSPAAQ
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCC
KYL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]