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The map label for this gene is vraA [H]

Identifier: 125624743

GI number: 125624743

Start: 1945938

End: 1947182

Strand: Direct

Name: vraA [H]

Synonym: llmg_1965

Alternate gene names: 125624743

Gene position: 1945938-1947182 (Clockwise)

Preceding gene: 125624722

Following gene: 125624753

Centisome position: 76.93

GC content: 32.21

Gene sequence:

>1245_bases
ATGGAACACAAAAATCAATTTAATTTCTCACATTTAGTTTCTCTTTATGCTGATAAAACAGCTTTAATTATCGGACCAGA
TAGATATACTTATCAGCAACTTGGAACTATGATTAAGAAAAGTGCTCAAAAATATAAAAATTCTTTTAAAAAAATTTTGA
TTGTAAAAAATGAAAACATCATAAATTTTATTACCGAATTTTTCGCAATTATTGAAAGTGGAAATTATCCCCTTGTGATA
GATTCTTTTACTCCCAAAACAAATATTGATAACCACTCTCTTCCCGATAACACCTTCTTTTTAGCTGCCACTTCTGGTAC
AACTGGTCAGCCTAAAATCTACTTACGAGACTGGCTTTCTTGGAAATCAGGCTTTGAAACAATTAATGACTTATTTCAGC
TCAAAAAAATTGAAAGTCTGGCAACTACCAGCCCTATGACTACCTCTTTGGGACTTCATAGTTTGGTTTGCTCCTTATAT
CTTGGTAAAAGCTTCCTCCCCATTACTAGTCCATCACAATTCGGCAAAATTAAAAAAAGTTGGGCTTTATTTACTGTTCC
AACCTATCTTTTGAATATTTTTAATGAACTCAGTGCTTTATCTTCTCTCAATACTTTATTTTTAAGCGGAGGAACTTTAA
GCCCAGAATCGATTGATAAAATAAAAAATCAACTTCCCTTAACAAAGATGATTGAATTTTATGGGAGCTCCGAAACTAGT
TTTATTTCTTGGCAATTTGTAGAAAATGGGAAAAAAACTGCAGCTGTCGGAAAACTTTTCCCGCATACACAAGTTGAATT
TGACGATAATCATAGCATCATTGTCAAAAGTCCTTATTTATTTTCTGGATATCTCAATCAAGCTAGCCCTCAGTCATGGA
CGACTGACGATTTAGGAACTATTAAAAATGACACTCTCTATTTATTTGGTAGACGTGCTGACATTATTGAACACAGGGCT
AACAAAATTTTTCCAGAAGAAATTGAGCAATTTGTAAAAGATTTATGTCAAGAATGTGCAGCTTTTGAAATATCCGATCA
AAAATATGGTCAGAAAATTGCGCTCTTAATTGTAAAACCACTAGAAAAAGAACTAGTGAAAGAAAGACTAAATCAAGAAC
TTCCAAAATATAAACGTCCACAAATTTATTTAGAAGCCGAAACACTCTCGCTAACTTCAAATCAAAAACTATCCAGAAAA
GAATTAGAACAAAACTACTTTAAAGGAATCTATCATGACTTTTAA

Upstream 100 bases:

>100_bases
TTTTTTTATTATACTGGTATCATATCAAATAGTAACCCTATAATCAAATTTTGGTTAACTGTTTTTAAAATTATAAGCTT
ATTGAATAAGGAAAACAGCA

Downstream 100 bases:

>100_bases
TAACTCTGTAGGAATAATTGCCGCAAATCGTTTACCAATTGGTAAAATAAATGGTTTTTATAAAAATATTTCCCCTGAAA
GTTTGTATCAGAATCTTATT

Product: putative AMP-binding enzyme

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEHKNQFNFSHLVSLYADKTALIIGPDRYTYQQLGTMIKKSAQKYKNSFKKILIVKNENIINFITEFFAIIESGNYPLVI
DSFTPKTNIDNHSLPDNTFFLAATSGTTGQPKIYLRDWLSWKSGFETINDLFQLKKIESLATTSPMTTSLGLHSLVCSLY
LGKSFLPITSPSQFGKIKKSWALFTVPTYLLNIFNELSALSSLNTLFLSGGTLSPESIDKIKNQLPLTKMIEFYGSSETS
FISWQFVENGKKTAAVGKLFPHTQVEFDDNHSIIVKSPYLFSGYLNQASPQSWTTDDLGTIKNDTLYLFGRRADIIEHRA
NKIFPEEIEQFVKDLCQECAAFEISDQKYGQKIALLIVKPLEKELVKERLNQELPKYKRPQIYLEAETLSLTSNQKLSRK
ELEQNYFKGIYHDF

Sequences:

>Translated_414_residues
MEHKNQFNFSHLVSLYADKTALIIGPDRYTYQQLGTMIKKSAQKYKNSFKKILIVKNENIINFITEFFAIIESGNYPLVI
DSFTPKTNIDNHSLPDNTFFLAATSGTTGQPKIYLRDWLSWKSGFETINDLFQLKKIESLATTSPMTTSLGLHSLVCSLY
LGKSFLPITSPSQFGKIKKSWALFTVPTYLLNIFNELSALSSLNTLFLSGGTLSPESIDKIKNQLPLTKMIEFYGSSETS
FISWQFVENGKKTAAVGKLFPHTQVEFDDNHSIIVKSPYLFSGYLNQASPQSWTTDDLGTIKNDTLYLFGRRADIIEHRA
NKIFPEEIEQFVKDLCQECAAFEISDQKYGQKIALLIVKPLEKELVKERLNQELPKYKRPQIYLEAETLSLTSNQKLSRK
ELEQNYFKGIYHDF
>Mature_414_residues
MEHKNQFNFSHLVSLYADKTALIIGPDRYTYQQLGTMIKKSAQKYKNSFKKILIVKNENIINFITEFFAIIESGNYPLVI
DSFTPKTNIDNHSLPDNTFFLAATSGTTGQPKIYLRDWLSWKSGFETINDLFQLKKIESLATTSPMTTSLGLHSLVCSLY
LGKSFLPITSPSQFGKIKKSWALFTVPTYLLNIFNELSALSSLNTLFLSGGTLSPESIDKIKNQLPLTKMIEFYGSSETS
FISWQFVENGKKTAAVGKLFPHTQVEFDDNHSIIVKSPYLFSGYLNQASPQSWTTDDLGTIKNDTLYLFGRRADIIEHRA
NKIFPEEIEQFVKDLCQECAAFEISDQKYGQKIALLIVKPLEKELVKERLNQELPKYKRPQIYLEAETLSLTSNQKLSRK
ELEQNYFKGIYHDF

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17558820, Length=351, Percent_Identity=25.3561253561254, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 47319; Mature: 47319

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKNQFNFSHLVSLYADKTALIIGPDRYTYQQLGTMIKKSAQKYKNSFKKILIVKNENI
CCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCH
INFITEFFAIIESGNYPLVIDSFTPKTNIDNHSLPDNTFFLAATSGTTGQPKIYLRDWLS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHH
WKSGFETINDLFQLKKIESLATTSPMTTSLGLHSLVCSLYLGKSFLPITSPSQFGKIKKS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
WALFTVPTYLLNIFNELSALSSLNTLFLSGGTLSPESIDKIKNQLPLTKMIEFYGSSETS
EEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCC
FISWQFVENGKKTAAVGKLFPHTQVEFDDNHSIIVKSPYLFSGYLNQASPQSWTTDDLGT
EEEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCC
IKNDTLYLFGRRADIIEHRANKIFPEEIEQFVKDLCQECAAFEISDQKYGQKIALLIVKP
CCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEECCCHHHCCEEEEEEECH
LEKELVKERLNQELPKYKRPQIYLEAETLSLTSNQKLSRKELEQNYFKGIYHDF
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEHKNQFNFSHLVSLYADKTALIIGPDRYTYQQLGTMIKKSAQKYKNSFKKILIVKNENI
CCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCH
INFITEFFAIIESGNYPLVIDSFTPKTNIDNHSLPDNTFFLAATSGTTGQPKIYLRDWLS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHH
WKSGFETINDLFQLKKIESLATTSPMTTSLGLHSLVCSLYLGKSFLPITSPSQFGKIKKS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
WALFTVPTYLLNIFNELSALSSLNTLFLSGGTLSPESIDKIKNQLPLTKMIEFYGSSETS
EEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCC
FISWQFVENGKKTAAVGKLFPHTQVEFDDNHSIIVKSPYLFSGYLNQASPQSWTTDDLGT
EEEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCC
IKNDTLYLFGRRADIIEHRANKIFPEEIEQFVKDLCQECAAFEISDQKYGQKIALLIVKP
CCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCEECCCHHHCCEEEEEEECH
LEKELVKERLNQELPKYKRPQIYLEAETLSLTSNQKLSRKELEQNYFKGIYHDF
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA