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The map label for this gene is bar [H]

Identifier: 125624737

GI number: 125624737

Start: 1940757

End: 1941317

Strand: Reverse

Name: bar [H]

Synonym: llmg_1959

Alternate gene names: 125624737

Gene position: 1941317-1940757 (Counterclockwise)

Preceding gene: 125624738

Following gene: 125624736

Centisome position: 76.75

GC content: 36.72

Gene sequence:

>561_bases
ATGGAGTTTAGACTAGCAAAAAAAGCTGATGCTCAGCGACTTTTAGAGATTTATAGACCATATGTTGAAAAAACAGCCAT
TACTTTTGAGTACGAAGTGCCTACACTAGAAGAATTTGAAAATCGAATTGAAAAAATTGGGAGTCAATTTCCTTATCTTT
TAGCGATTGAAAATGGTAAAATCATTGGTTATGCCTATGCAGGGGCCTACCGTGAACGGGCGGCTTATGATTGGGTGGTC
GAATTATCAATTTATTTAGATGAAAAAGAGCGACAACATGGAGCTGGAAGTGCCCTTTATCAAAAGTTACTGACAGCCTT
AGCGGCACTCAATTATCAACGGGCCTATGCTTGTATTACTTATCCAAATCCAGCTAGTGTCGCTTTCCATGAGAAATTTG
GATTTCAACAAATTGGACTTTTCCCCAAGGCTGGTTATAAGTTTGAACAATGGTATGGAATTGTTTGGTTAGAATTAGCA
CTACAAAAATCTCAAGAGGTAAAGGCAACAAAATTACTGACAGATTTATCAGAAGAGGAAATAAAAAAAATCTTGGAATA
A

Upstream 100 bases:

>100_bases
CTTTTATTTATTTTTAAAATTTTATTATCTAGCGATGTGAGAAGTTCTGTCATTAGTTAATAATTGGTGTATAATAGATA
AAAACTATAAAGGGGATAAG

Downstream 100 bases:

>100_bases
TGACCAAGGTTTTTTTATTATATAAATTATAAAAGAAGTAAAACATTTTGCCTTTTGCAAGAAAAACATTAAAATAAAAT
TAAAGAAAATAAATATAAAT

Product: phosphinothricin N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MEFRLAKKADAQRLLEIYRPYVEKTAITFEYEVPTLEEFENRIEKIGSQFPYLLAIENGKIIGYAYAGAYRERAAYDWVV
ELSIYLDEKERQHGAGSALYQKLLTALAALNYQRAYACITYPNPASVAFHEKFGFQQIGLFPKAGYKFEQWYGIVWLELA
LQKSQEVKATKLLTDLSEEEIKKILE

Sequences:

>Translated_186_residues
MEFRLAKKADAQRLLEIYRPYVEKTAITFEYEVPTLEEFENRIEKIGSQFPYLLAIENGKIIGYAYAGAYRERAAYDWVV
ELSIYLDEKERQHGAGSALYQKLLTALAALNYQRAYACITYPNPASVAFHEKFGFQQIGLFPKAGYKFEQWYGIVWLELA
LQKSQEVKATKLLTDLSEEEIKKILE
>Mature_186_residues
MEFRLAKKADAQRLLEIYRPYVEKTAITFEYEVPTLEEFENRIEKIGSQFPYLLAIENGKIIGYAYAGAYRERAAYDWVV
ELSIYLDEKERQHGAGSALYQKLLTALAALNYQRAYACITYPNPASVAFHEKFGFQQIGLFPKAGYKFEQWYGIVWLELA
LQKSQEVKATKLLTDLSEEEIKKILE

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=173, Percent_Identity=28.9017341040462, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 21557; Mature: 21557

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRLAKKADAQRLLEIYRPYVEKTAITFEYEVPTLEEFENRIEKIGSQFPYLLAIENGK
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCC
IIGYAYAGAYRERAAYDWVVELSIYLDEKERQHGAGSALYQKLLTALAALNYQRAYACIT
EEEEEECCHHHHHHHHHEEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
YPNPASVAFHEKFGFQQIGLFPKAGYKFEQWYGIVWLELALQKSQEVKATKLLTDLSEEE
CCCCCCEEEHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
IKKILE
HHHHCC
>Mature Secondary Structure
MEFRLAKKADAQRLLEIYRPYVEKTAITFEYEVPTLEEFENRIEKIGSQFPYLLAIENGK
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCC
IIGYAYAGAYRERAAYDWVVELSIYLDEKERQHGAGSALYQKLLTALAALNYQRAYACIT
EEEEEECCHHHHHHHHHEEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
YPNPASVAFHEKFGFQQIGLFPKAGYKFEQWYGIVWLELALQKSQEVKATKLLTDLSEEE
CCCCCCEEEHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
IKKILE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3240868; 8593056 [H]