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The map label for this gene is exoA [H]

Identifier: 125624555

GI number: 125624555

Start: 1750191

End: 1751048

Strand: Direct

Name: exoA [H]

Synonym: llmg_1765

Alternate gene names: 125624555

Gene position: 1750191-1751048 (Clockwise)

Preceding gene: 125624551

Following gene: 125624556

Centisome position: 69.19

GC content: 40.09

Gene sequence:

>858_bases
ATGAACTATAAATTTATCTCATGGAATATTGACTCACTCAATGCTGCATTGACTGGAACCTCAGAACGTGCTGCCCTTTC
TCTTGCTGTTGTTAAAAATCTTGCCCAAGCACAACCAGACGTTTTAGCCATTCAAGAAACCAAACTTCCTTCTACTGGAC
CTAAAAAAGCTCATTTGGAAGTTTTGGAAGAACTGTTTAAAGGTTATAAAATCGTCTGGCGTTCATCAGTAGAACCTGCT
CGAAAAGGATATTCTGGAACAATGGTTCTTTATAAAGAAACTCTACCAGAACCAGTCATCACTTTTCCTGAAATTGATGC
TCCTGAACCTATGGATGCCGAAGGTCGAATCATCACCCTTGAATTTCCTGATTTCTTTGTCACTACGGTTTATACACCAA
ATGCGCAAGAAGGCCTGACACGACTTGATTTACGTGGAATTTGGGATGATAACTATCGTAACTATCTCACAAGCCTTGAT
GCTCAAAAACCTGTCTTTGCTTGTGGAGATTTCAATGCGGCCTATACAGAAATCGACTTAGCCAATCCCCGTGGTAATCA
CAACTCAGCTGGATTTACTGACCAAGAGCGTGAAAAATTTGGTCAACTTTTAGAGGCTGGATTTACTGATACATTTCGTC
AACTCCATGGTCAAGTTGAGAAATTTTATGAAGAAGGACGTAGTATTTATACTTGGTTTGCCCAACGTGCAAAAACTTCA
AAACTAAATAATTCAGGTTGGAGAATTGACTACTGGCTGGTTTCCAATCGTCTTGCCCAAGCAGTTAAAGTTTCTGAACC
CCTTGATTCTGGTGAACGTTTAGACCACGTCCCCATTCTCTTAGAATTTGAACTCTAA

Upstream 100 bases:

>100_bases
GCATTGTCTGATTAAGTTTGTTATAATTATCATAGAAAAGTTAAGAATTCCTTTAAGTCACAGCTCTTTGGAAATTCTTT
TAAAAAATAAAGGAACGATT

Downstream 100 bases:

>100_bases
AATAAAAGAACCTTAAAGGTTCTTTTTTATTGTCAATAAAACAAATAGAGACTATAATTGAGTAAAAATAGTTGAGGAGA
ACATGTTTATGGTTAATATA

Product: 3'-exo-deoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNYKFISWNIDSLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPA
RKGYSGTMVLYKETLPEPVITFPEIDAPEPMDAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLD
AQKPVFACGDFNAAYTEIDLANPRGNHNSAGFTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTS
KLNNSGWRIDYWLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL

Sequences:

>Translated_285_residues
MNYKFISWNIDSLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPA
RKGYSGTMVLYKETLPEPVITFPEIDAPEPMDAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLD
AQKPVFACGDFNAAYTEIDLANPRGNHNSAGFTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTS
KLNNSGWRIDYWLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL
>Mature_285_residues
MNYKFISWNIDSLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLEVLEELFKGYKIVWRSSVEPA
RKGYSGTMVLYKETLPEPVITFPEIDAPEPMDAEGRIITLEFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLD
AQKPVFACGDFNAAYTEIDLANPRGNHNSAGFTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTS
KLNNSGWRIDYWLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=283, Percent_Identity=36.3957597173145, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI18375503, Length=283, Percent_Identity=36.3957597173145, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI18375501, Length=283, Percent_Identity=36.3957597173145, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788046, Length=298, Percent_Identity=29.5302013422819, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71989536, Length=285, Percent_Identity=35.0877192982456, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71989539, Length=165, Percent_Identity=29.6969696969697, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330655, Length=286, Percent_Identity=34.2657342657343, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17136678, Length=286, Percent_Identity=34.2657342657343, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32295; Mature: 32295

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKFISWNIDSLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLE
CCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
VLEELFKGYKIVWRSSVEPARKGYSGTMVLYKETLPEPVITFPEIDAPEPMDAEGRIITL
HHHHHHCCEEEEECCCCCHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEE
EFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDL
ECCCEEEEEEECCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEE
ANPRGNHNSAGFTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTS
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLNNSGWRIDYWLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL
CCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MNYKFISWNIDSLNAALTGTSERAALSLAVVKNLAQAQPDVLAIQETKLPSTGPKKAHLE
CCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
VLEELFKGYKIVWRSSVEPARKGYSGTMVLYKETLPEPVITFPEIDAPEPMDAEGRIITL
HHHHHHCCEEEEECCCCCHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEE
EFPDFFVTTVYTPNAQEGLTRLDLRGIWDDNYRNYLTSLDAQKPVFACGDFNAAYTEIDL
ECCCEEEEEEECCCHHHCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCEEEEEEE
ANPRGNHNSAGFTDQEREKFGQLLEAGFTDTFRQLHGQVEKFYEEGRSIYTWFAQRAKTS
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLNNSGWRIDYWLVSNRLAQAVKVSEPLDSGERLDHVPILLEFEL
CCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]