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The map label for this gene is gshR [H]

Identifier: 125624497

GI number: 125624497

Start: 1676943

End: 1678250

Strand: Reverse

Name: gshR [H]

Synonym: llmg_1702

Alternate gene names: 125624497

Gene position: 1678250-1676943 (Counterclockwise)

Preceding gene: 125624498

Following gene: 125624495

Centisome position: 66.35

GC content: 34.94

Gene sequence:

>1308_bases
ATGTTTGATTATATTATTATTGGAGCAGGACCTGGGGGACTTGGCTTAGCTTATCGCCTTAAAACGAAAGATAATAAAAT
TGCAATGATTGAAAATGATAAGTGGGGTGGAACTTGTCCAAATTATGGTTGTGACCCAACTAAAATGATGATGGCAGTGA
TTGAGGCTAAAAGTCATGTTGAACATTTAAAAGGTCAAGGAATTTCGGGTGATTTAAAGATTGATTGGAAAGGTTTGCGA
AGTCGTAAGTTAAATATTACCGATCCTTACGAAAAATCGACTTTTGAAGGGTTAAAAAATGCTGATATTGAAACGATATA
TGGTAGCGCAAGTTTTACTGAGCAAGGAGAACTGCAAGTTGCAGGAGAGATTTATCAGGCCAAAACTTATATTATTGCGG
CAGGAAGTCGTCCACGTCGTCTAGAACTTGATGGTGACGAATTTCTAAAAACAAGTAATGATTTTTTAGCTCTTGAAGAA
TTACCTGCTAAGATTAGTTTCATGGGGAGTGGGCCAATATCATTAGAATTAGCTCAAATTGCTAAAGCTGCTGGAAGTGA
TGTAACGATTATTTCTCGTAAGAAAAGTAATGTTGCTCATTTTGATGAAGAAATGGGACAAGAATTTATCAATTACCTCA
AAAATCAGGGCATTAATTTTATTGAAAATATTTCTGTAAGCAAAGTTGAAAAATTTACTGACGGTTTTTTGTTGACTGAT
GGAGCTGGTTTTGAACATAAAACGGATTTAGTGATTGCCGGAGTTGGTCGTCAGCCAAATAGCGATCAGCTTAATTTAGA
TAAAGTTGGCGTTGAAACAGACCACAAAGGGATTAAAGTAAACGACTATCTACAAACAAGCAATCCTAAAATTTATGCCA
TGGGAGATGTCCTCTCTAAAGCGGAGCCTCATTTAACTCCTGTTTCTTCATTTGAAGGAAATTATTTGGGTCAAAATTTG
CCAAATGCTAATAAAGTACCAATCCATTATCCACAGATTCCGACAATTATTTTTGGAACGGCAAAATTAGCTGAGGTGGG
GATTTTGACAGGGACTGGAATTCGCACAAAAAGTTTGAATTTAAAATCTTGGTATACTTATAAGAGAATTAATGACCCCT
TAGCAAAACTAAAAGTTGCCATCAATGAAAATCAAGAAATTGTCGGTGCTTCAACAGTAAGTTCAGTAGCTGATGAAGTG
ATTAATTTAATCAGTATTTTAATTCAACAAAAAATGACATTGGCTGATGTTGAAAAAATGATTTTTACTTATCCAACAGT
AGCTAGTGACTTAGAATATTTTTACTGA

Upstream 100 bases:

>100_bases
GCTAAATAGTTTGATATTTTGGAGAAAAAAAGTAGAATTAATCTAAAGCCTTGTAAGTTATTACATTAAAAGTTTAATTT
GAAAATTAGAAGGAGAAAGA

Downstream 100 bases:

>100_bases
CAGTTTGCTTGGCTGATGTGTAACATTATAATAGAAGAAACCATAAGTCAAAGCTGAAGAAAATTTTTTACTGACAGAAT
ATATTTTCAAGTGGTCAGTA

Product: glutathione reductase

Products: NA

Alternate protein names: GR; GRase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIAMIENDKWGGTCPNYGCDPTKMMMAVIEAKSHVEHLKGQGISGDLKIDWKGLR
SRKLNITDPYEKSTFEGLKNADIETIYGSASFTEQGELQVAGEIYQAKTYIIAAGSRPRRLELDGDEFLKTSNDFLALEE
LPAKISFMGSGPISLELAQIAKAAGSDVTIISRKKSNVAHFDEEMGQEFINYLKNQGINFIENISVSKVEKFTDGFLLTD
GAGFEHKTDLVIAGVGRQPNSDQLNLDKVGVETDHKGIKVNDYLQTSNPKIYAMGDVLSKAEPHLTPVSSFEGNYLGQNL
PNANKVPIHYPQIPTIIFGTAKLAEVGILTGTGIRTKSLNLKSWYTYKRINDPLAKLKVAINENQEIVGASTVSSVADEV
INLISILIQQKMTLADVEKMIFTYPTVASDLEYFY

Sequences:

>Translated_435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIAMIENDKWGGTCPNYGCDPTKMMMAVIEAKSHVEHLKGQGISGDLKIDWKGLR
SRKLNITDPYEKSTFEGLKNADIETIYGSASFTEQGELQVAGEIYQAKTYIIAAGSRPRRLELDGDEFLKTSNDFLALEE
LPAKISFMGSGPISLELAQIAKAAGSDVTIISRKKSNVAHFDEEMGQEFINYLKNQGINFIENISVSKVEKFTDGFLLTD
GAGFEHKTDLVIAGVGRQPNSDQLNLDKVGVETDHKGIKVNDYLQTSNPKIYAMGDVLSKAEPHLTPVSSFEGNYLGQNL
PNANKVPIHYPQIPTIIFGTAKLAEVGILTGTGIRTKSLNLKSWYTYKRINDPLAKLKVAINENQEIVGASTVSSVADEV
INLISILIQQKMTLADVEKMIFTYPTVASDLEYFY
>Mature_435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIAMIENDKWGGTCPNYGCDPTKMMMAVIEAKSHVEHLKGQGISGDLKIDWKGLR
SRKLNITDPYEKSTFEGLKNADIETIYGSASFTEQGELQVAGEIYQAKTYIIAAGSRPRRLELDGDEFLKTSNDFLALEE
LPAKISFMGSGPISLELAQIAKAAGSDVTIISRKKSNVAHFDEEMGQEFINYLKNQGINFIENISVSKVEKFTDGFLLTD
GAGFEHKTDLVIAGVGRQPNSDQLNLDKVGVETDHKGIKVNDYLQTSNPKIYAMGDVLSKAEPHLTPVSSFEGNYLGQNL
PNANKVPIHYPQIPTIIFGTAKLAEVGILTGTGIRTKSLNLKSWYTYKRINDPLAKLKVAINENQEIVGASTVSSVADEV
INLISILIQQKMTLADVEKMIFTYPTVASDLEYFY

Specific function: Maintains High Levels Of Reduced Glutathione In The Cytosol. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=28.4463894967177, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=26.4367816091954, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=26.2068965517241, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=26.2068965517241, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=26.2068965517241, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=26.2068965517241, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=26.2068965517241, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI22035672, Length=431, Percent_Identity=26.4501160092807, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=326, Percent_Identity=29.1411042944785, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=24.8908296943231, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=28.538283062645, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=26.7857142857143, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI87082354, Length=450, Percent_Identity=26.2222222222222, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=24.4541484716157, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789065, Length=232, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=29.8901098901099, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=29.2682926829268, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=25.8547008547009, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=24.2424242424242, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=433, Percent_Identity=25.4041570438799, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=26.0869565217391, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321091, Length=463, Percent_Identity=24.622030237581, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=22.2929936305732, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640553, Length=464, Percent_Identity=26.7241379310345, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24640549, Length=464, Percent_Identity=26.7241379310345, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24640551, Length=464, Percent_Identity=26.7241379310345, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=25.1599147121535, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=26.0869565217391, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006324
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.7 [H]

Molecular weight: Translated: 47838; Mature: 47838

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYIIIGAGPGGLGLAYRLKTKDNKIAMIENDKWGGTCPNYGCDPTKMMMAVIEAKSHV
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EHLKGQGISGDLKIDWKGLRSRKLNITDPYEKSTFEGLKNADIETIYGSASFTEQGELQV
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCEEE
AGEIYQAKTYIIAAGSRPRRLELDGDEFLKTSNDFLALEELPAKISFMGSGPISLELAQI
ECEEEEEEEEEEECCCCCCEEECCCHHHHHCCCCEEEEECCCCEEEEECCCCCEEEHHHH
AKAAGSDVTIISRKKSNVAHFDEEMGQEFINYLKNQGINFIENISVSKVEKFTDGFLLTD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCEEEEC
GAGFEHKTDLVIAGVGRQPNSDQLNLDKVGVETDHKGIKVNDYLQTSNPKIYAMGDVLSK
CCCCCCCCCEEEEECCCCCCCCCCCHHEECCCCCCCCEEEEEEEECCCCEEEEECHHHHC
AEPHLTPVSSFEGNYLGQNLPNANKVPIHYPQIPTIIFGTAKLAEVGILTGTGIRTKSLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHEEEEECCCCEEEECC
LKSWYTYKRINDPLAKLKVAINENQEIVGASTVSSVADEVINLISILIQQKMTLADVEKM
CCCEEEHHHHCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IFTYPTVASDLEYFY
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MFDYIIIGAGPGGLGLAYRLKTKDNKIAMIENDKWGGTCPNYGCDPTKMMMAVIEAKSHV
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EHLKGQGISGDLKIDWKGLRSRKLNITDPYEKSTFEGLKNADIETIYGSASFTEQGELQV
HHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEEECCCCCCCCCCEEE
AGEIYQAKTYIIAAGSRPRRLELDGDEFLKTSNDFLALEELPAKISFMGSGPISLELAQI
ECEEEEEEEEEEECCCCCCEEECCCHHHHHCCCCEEEEECCCCEEEEECCCCCEEEHHHH
AKAAGSDVTIISRKKSNVAHFDEEMGQEFINYLKNQGINFIENISVSKVEKFTDGFLLTD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCEEEEC
GAGFEHKTDLVIAGVGRQPNSDQLNLDKVGVETDHKGIKVNDYLQTSNPKIYAMGDVLSK
CCCCCCCCCEEEEECCCCCCCCCCCHHEECCCCCCCCEEEEEEEECCCCEEEEECHHHHC
AEPHLTPVSSFEGNYLGQNLPNANKVPIHYPQIPTIIFGTAKLAEVGILTGTGIRTKSLN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHEEEEECCCCEEEECC
LKSWYTYKRINDPLAKLKVAINENQEIVGASTVSSVADEVINLISILIQQKMTLADVEKM
CCCEEEHHHHCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IFTYPTVASDLEYFY
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7538273 [H]