Click here to switch to the map view.

The map label for this gene is recN [H]

Identifier: 125624483

GI number: 125624483

Start: 1660156

End: 1661823

Strand: Reverse

Name: recN [H]

Synonym: llmg_1685

Alternate gene names: 125624483

Gene position: 1661823-1660156 (Counterclockwise)

Preceding gene: 125624484

Following gene: 125624482

Centisome position: 65.7

GC content: 34.59

Gene sequence:

>1668_bases
ATGTTACAAGAGATTTCAATCAAAAATTTTGCAATTATTGAAGAAATTCATCTTTCATTTGAAAGTGGAATGACTATTTT
AACTGGGGAAACAGGGGCTGGAAAATCAATTATTATTGATGCCATGTCCTTATTATTAGGTGGACGTGCATCAAGTGATT
TCGTGCGTCATGGCGCTAGTAAAGCCGAAATTGAGGGGCTCTTTTTCTTTGAAAAAACACCAGAATTAAATTCTGCTTTA
TTAGAATTGGGCTTTGAAGAATTAGATTCTGAAATTATTTTGCGACGTGAAATTTTTGCGAATGGTCGCTCTGTCTGTCG
AATTAATGGACAAATGGTCAATTTAACAAGACTTCGTCAAATTGGAGAATTTTTAGTAGACATTCATGGTCAACATGACA
GTCAAGAACTGATGAATCCCAAAAGTCATCTTCGTTTATTGGATGAGTTTGGAGATGAAAATTTTGAAGTCATTAAAAAT
AATTACAAAAATAAATTTGAAAACTTTAAAAATCTTCGGCAACAATTGAATATTCGTCAAAAAAATGAACAAGAATTTGC
CCAAAGAATTGAAATTCTCCAATTTCAGGCTGAAGAAATTGAAGCAGCAGAAATTAATCTTGAAGAAGATGAGCTTTTAG
TTAATCGCCGTGAAAAATTAAATAATATAAAAAATATTGCGGATTCTCTAAGTACGGCATATTTAGCACTAGAAGATGAA
GACAACGACTATTCAAGCCTGAATAATATTCGGACGACAATGACCGAATTGGATAGAATTGGCAATTTTGATAAGGATTA
TCAAGAACTTGCTGACAAAACTGCTGAAAGTTATTATATATTAGAAGAAGTTGCCAATCAAATTCAAAGCATCATGAGTG
ATTTGGAATTCAACCCAGCGGAACTCTTGCAGATTGAAGACCGTATTATGACACTGACAACTTTGAGAAAAAAATATGGC
CCTACACTTTCTGATGTCATCAATTATTTGGAAAAAGTTCAACTTGAACTTTCAGAACTCACAGGCTCAGAAAATGATTC
CGTCAGTCTGGAAAAATCAGTTAAAAATGCCCAAGTTGAACTCCTTGAAGCGAGCCAAGAACTCAATAAAGCCCGTCATC
AAATCGCCAAAGAAATTGAAAAAGATATCAAGCATGAACTTTCAGAACTTTATATGGAAAAGGCTGATTTTAAAGTCAGT
TTTGATAGTGCGAAATTTTCAGCAAATGGTAATGAGCATGTCGAATTTTTCATTCAAACTAATCCAGGTGAGGGTTTCAA
ACCATTGGCAAAAACCGCTTCTGGTGGTGAACTTTCACGTTTAATGCTTGCAATAAAATCAAGCTTTTCAAGACGTGAAA
ATAAAACAGCCATTGTTTTTGATGAAGTTGATACAGGAGTTTCTGGGCGTGTGGCTCAAGCAATTGCCAATAAAATACAT
AAAATTGCCAGTCATGGACAAGTTCTTGCGATTTCTCATCTCCCCCAAGTAGTGGCAATTGCTGACAGACAATTTTATAT
TGAAAAAGCTTCGACTGATGATATTACAACCTCGACTGTGCGAAAATTGAATTTTGACGAACGAGTAAAAGAGATTGCAA
AAATGCTTGCTGGTGAAGATTTAACTGAAGAAGCACTTGCTCAAGCAAAACGTTTGTTAACTAATTAA

Upstream 100 bases:

>100_bases
CTGTCAGTAAAAAATATAATGATAGAGATGCCCAAAAGGACAAGTCAAGGCTGTTGGAGTACTGACAGCCTTGTTAATTA
TTTTTGAGAAAGGAAATTTG

Downstream 100 bases:

>100_bases
AGATAATCTTGGCCTTGGTCAAGGTTTTTTTATTTTTACAATTTTAATGATATGCATAAAAAAGAAATATTGCAACCACA
AATGTGTATATACCAAAACA

Product: DNA repair protein recN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGASKAEIEGLFFFEKTPELNSAL
LELGFEELDSEIILRREIFANGRSVCRINGQMVNLTRLRQIGEFLVDIHGQHDSQELMNPKSHLRLLDEFGDENFEVIKN
NYKNKFENFKNLRQQLNIRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDELLVNRREKLNNIKNIADSLSTAYLALEDE
DNDYSSLNNIRTTMTELDRIGNFDKDYQELADKTAESYYILEEVANQIQSIMSDLEFNPAELLQIEDRIMTLTTLRKKYG
PTLSDVINYLEKVQLELSELTGSENDSVSLEKSVKNAQVELLEASQELNKARHQIAKEIEKDIKHELSELYMEKADFKVS
FDSAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIH
KIASHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNFDERVKEIAKMLAGEDLTEEALAQAKRLLTN

Sequences:

>Translated_555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGASKAEIEGLFFFEKTPELNSAL
LELGFEELDSEIILRREIFANGRSVCRINGQMVNLTRLRQIGEFLVDIHGQHDSQELMNPKSHLRLLDEFGDENFEVIKN
NYKNKFENFKNLRQQLNIRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDELLVNRREKLNNIKNIADSLSTAYLALEDE
DNDYSSLNNIRTTMTELDRIGNFDKDYQELADKTAESYYILEEVANQIQSIMSDLEFNPAELLQIEDRIMTLTTLRKKYG
PTLSDVINYLEKVQLELSELTGSENDSVSLEKSVKNAQVELLEASQELNKARHQIAKEIEKDIKHELSELYMEKADFKVS
FDSAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIH
KIASHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNFDERVKEIAKMLAGEDLTEEALAQAKRLLTN
>Mature_555_residues
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGASKAEIEGLFFFEKTPELNSAL
LELGFEELDSEIILRREIFANGRSVCRINGQMVNLTRLRQIGEFLVDIHGQHDSQELMNPKSHLRLLDEFGDENFEVIKN
NYKNKFENFKNLRQQLNIRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDELLVNRREKLNNIKNIADSLSTAYLALEDE
DNDYSSLNNIRTTMTELDRIGNFDKDYQELADKTAESYYILEEVANQIQSIMSDLEFNPAELLQIEDRIMTLTTLRKKYG
PTLSDVINYLEKVQLELSELTGSENDSVSLEKSVKNAQVELLEASQELNKARHQIAKEIEKDIKHELSELYMEKADFKVS
FDSAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIH
KIASHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNFDERVKEIAKMLAGEDLTEEALAQAKRLLTN

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=565, Percent_Identity=33.4513274336283, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62871; Mature: 62871

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGAS
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFFFEKTPELNSALLELGFEELDSEIILRREIFANGRSVCRINGQMVNLTRLRQ
HHHCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHH
IGEFLVDIHGQHDSQELMNPKSHLRLLDEFGDENFEVIKNNYKNKFENFKNLRQQLNIRQ
HHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KNEQEFAQRIEILQFQAEEIEAAEINLEEDELLVNRREKLNNIKNIADSLSTAYLALEDE
CCHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
DNDYSSLNNIRTTMTELDRIGNFDKDYQELADKTAESYYILEEVANQIQSIMSDLEFNPA
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELLQIEDRIMTLTTLRKKYGPTLSDVINYLEKVQLELSELTGSENDSVSLEKSVKNAQVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LLEASQELNKARHQIAKEIEKDIKHELSELYMEKADFKVSFDSAKFSANGNEHVEFFIQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCEEEEEEEE
NPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIH
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
KIASHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNFDERVKEIAKMLAGED
HHHCCCCEEEEHHCCHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC
LTEEALAQAKRLLTN
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLLLGGRASSDFVRHGAS
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFFFEKTPELNSALLELGFEELDSEIILRREIFANGRSVCRINGQMVNLTRLRQ
HHHCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHH
IGEFLVDIHGQHDSQELMNPKSHLRLLDEFGDENFEVIKNNYKNKFENFKNLRQQLNIRQ
HHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KNEQEFAQRIEILQFQAEEIEAAEINLEEDELLVNRREKLNNIKNIADSLSTAYLALEDE
CCHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
DNDYSSLNNIRTTMTELDRIGNFDKDYQELADKTAESYYILEEVANQIQSIMSDLEFNPA
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
ELLQIEDRIMTLTTLRKKYGPTLSDVINYLEKVQLELSELTGSENDSVSLEKSVKNAQVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LLEASQELNKARHQIAKEIEKDIKHELSELYMEKADFKVSFDSAKFSANGNEHVEFFIQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCEEEEEEEE
NPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAIVFDEVDTGVSGRVAQAIANKIH
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHH
KIASHGQVLAISHLPQVVAIADRQFYIEKASTDDITTSTVRKLNFDERVKEIAKMLAGED
HHHCCCCEEEEHHCCHHHHHHCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHCCCC
LTEEALAQAKRLLTN
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]