Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 125624346

GI number: 125624346

Start: 1514411

End: 1516579

Strand: Direct

Name: nrdE [H]

Synonym: llmg_1543

Alternate gene names: 125624346

Gene position: 1514411-1516579 (Clockwise)

Preceding gene: 125624345

Following gene: 125624347

Centisome position: 59.87

GC content: 35.78

Gene sequence:

>2169_bases
ATGTCTCTAAAACAACTTAAAGATGTAACTTATTTCAAGCTCAATAACGAGATTAACATCCCCGTGAATGGTAGCATTCC
TCTTGCCAAAGATAAAGAAGCAATTACCGCTTTTTTCAAAGAGAATGTTGAACCTAATCGTTATGAAGCTGACACTTATA
TCGATAAACTTAATTGGTTAGTTAAAGAAGAATACCTAGAAGCTGAATTTCTAGAAAAATATAAGCCAGAATTTATCACA
AATCTTCGTAAAGAATTGAGTAAATTTGAATTTCATTTTGATTCATTTATGGCTGCTTATAAATTTTATAATCAATATGC
CATGAAAAACAATGACGGTTCACTCTATCTTGAAGATTTTGAAGACCGTGTGTTAATGAATGCTTTATACATGGCTGACG
GTGATGAAAAATTAGCTAATAATCTTGCTTTTGCAATGATTAATCGTCGTTATCAACCGGCTACTCCAACTTTCTTAAAT
GCTGGACGTAAACGTCGTGGTGAATTCGTTTCTTGTTTTCTTTTACAATTGACTGATGATATGAATTCAATTGGGCGGAC
GATTAATTCTGCCTTACAACTTTCTAAAAATGGTGGTGGTGTTGGTCTTAATTTGACTAATCTGCGTGCTGCTGGTGCAC
CAATCAAAGGATATGATGGAGTTGCCGCTGGTGTTGTGCCTGTCATGAAACTTTTTGAAGATGCTTTTTCTTATGCCAAT
CAATTGGGGCAACGACAAGGTGCTGGTGTAGTTTATTTATCACTTTTCCATCCAGATATCATGGAATTTTTATCAACTAA
AAAAGAAAATGCTGACGAAAAAATCCGCGTTAAAACACTCTCACTTGGTGTTACTGTTCCTGATAAATTCTACGAATTGG
TTAAAAAAGGCGAAACAATGTATCTTTTTGAACCCTACTTCGTTGAAAAATACTATGGTAAACCTTTTGCCGAAGTCGAT
ATTACAGCTGAATACGACAAGATGGTGGCTAACCCAGAAATTCGCAAGACACCAATTAATGCCCGTGAGCTTGAACAAGA
ACTTTCAAAACTTCAACAAGAATCTGGTTATCCTTATATCGTGAATGTCGATACGGTCAATCAAGCTAATCCTGTTGATG
GGGTGATTTCAATGTCAAACCTTTGTTCTGAAATCCTTCAAGTTCAAACACCTTCTGTTTTGAATGATGACCAAACTTAT
AAAGTCGTTGGAACTGACGTTGCTTGTAACTTAGGTTCAACTAATGTCTTAAATATGATGACTTCCAGTGATGAATTTGG
AACTTCTGTTGAATCAATGGTTCGTGCTTTAACTTTCATTTCTGAAACTTCAAACCTGGATACTGTTCCAACTGTTCGTA
AAGGAAATGATGAAATGCATGCCATTGGGCTTGGAGCAATGGGCTTGCATTCTTTCTTAGCTCAAAATCAAATTCATTAT
GATAGTAAAGAAGCCGTTGAATTCACAAGTGTCTATTTCATGTTGTTGAATTACTACACTTTGGTGGCTTCAAATAAATT
AGCTGTTGAAAAAGGGTCTAGCTTCAAGAATTTTGAAAAATCGGCCTATGCTGATGGTTCTTATTTTGATAAATACTTGA
CTCATGATTATTTTGCAGCTGTTTCTCCTAAAGTTCAAGAACTTTTCAAAGGAATTGCCGTTCCAACAATCGCTGATTGG
ACTGAATTACGCGATACTGTAAAAACTTCTGGTCTTTATAATTCTTATCGTATGGCTGTAGCTCCAAATGGTTCTATTTC
TTATATCAATGACTGCTCTTCTTCTATCCACCCAATCGTCAATCGGATTGAAGAACGTCAAGAAAAGAAAGTTGGGAAAA
TTTATTATCCAGCTGCTGGACTTGCCACTGAAACAATTCCTTACTACAAATCCGCTTATGATACTGATATGCGCGCGGTC
ATTGATGTTTATGCGGCTGCTACAGAACACGTTGACCAAGGATTGTCACTCACATTATTTATGCGTTCAACTTTACCAGA
AGGTTTGTATGAATGGAAAGTTGCAAATAATAAAATGACCACACGTGATTTGTCTATTTTGCGTAACTATGCTTACAAAA
AAGGAATTAAAACAATTTATTACATTCGTACATTTACTGATGACCAAGAAGAAGTCGGCGCTAACCAATGTGAAAGTTGT
GTCATTTAA

Upstream 100 bases:

>100_bases
TTATTTTAACTTTTATTTTATAATAACAAGTTTGAACTTATCATACATTTCAGATTCACTTATTTTTAAAAGTAAGTGAA
TACTTTATAAAAGGAGTATT

Downstream 100 bases:

>100_bases
TAAAACATAAACAATGATGCTAAAGCACCTATTGTTTATACGAAAGCCGCTAGAGCGGACTTTCTATTTTCTTACTTGTT
TCATTTTTCTGAAAAATCGT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFIT
NLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLN
AGRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVD
ITAEYDKMVANPEIRKTPINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTY
KVVGTDVACNLGSTNVLNMMTSSDEFGTSVESMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSPKVQELFKGIAVPTIADW
TELRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAV
IDVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
VI

Sequences:

>Translated_722_residues
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFIT
NLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLN
AGRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVD
ITAEYDKMVANPEIRKTPINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTY
KVVGTDVACNLGSTNVLNMMTSSDEFGTSVESMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSPKVQELFKGIAVPTIADW
TELRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAV
IDVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
VI
>Mature_721_residues
SLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWLVKEEYLEAEFLEKYKPEFITN
LRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDFEDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNA
GRKRRGEFVSCFLLQLTDDMNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYANQ
LGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETMYLFEPYFVEKYYGKPFAEVDI
TAEYDKMVANPEIRKTPINARELEQELSKLQQESGYPYIVNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYK
VVGTDVACNLGSTNVLNMMTSSDEFGTSVESMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHYD
SKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAAVSPKVQELFKGIAVPTIADWT
ELRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIVNRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVI
DVYAAATEHVDQGLSLTLFMRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESCV
I

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=602, Percent_Identity=26.9102990033223, Blast_Score=167, Evalue=4e-41,
Organism=Escherichia coli, GI1789030, Length=715, Percent_Identity=49.7902097902098, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=569, Percent_Identity=27.4165202108963, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=647, Percent_Identity=26.7387944358578, Blast_Score=189, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320914, Length=614, Percent_Identity=27.5244299674267, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=619, Percent_Identity=27.4636510500808, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17352481, Length=630, Percent_Identity=26.031746031746, Blast_Score=166, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81519; Mature: 81388

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWL
CCHHHHCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VKEEYLEAEFLEKYKPEFITNLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCH
EDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNAGRKRRGEFVSCFLLQLTDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHH
MNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
HHHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEE
YLFEPYFVEKYYGKPFAEVDITAEYDKMVANPEIRKTPINARELEQELSKLQQESGYPYI
EEECHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYKVVGTDVACNLGSTNVLNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHC
TSSDEFGTSVESMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
CCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
VSPKVQELFKGIAVPTIADWTELRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHH
NRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVIDVYAAATEHVDQGLSLTLF
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
EHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHC
VI
CC
>Mature Secondary Structure 
SLKQLKDVTYFKLNNEINIPVNGSIPLAKDKEAITAFFKENVEPNRYEADTYIDKLNWL
CHHHHCCCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
VKEEYLEAEFLEKYKPEFITNLRKELSKFEFHFDSFMAAYKFYNQYAMKNNDGSLYLEDF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCH
EDRVLMNALYMADGDEKLANNLAFAMINRRYQPATPTFLNAGRKRRGEFVSCFLLQLTDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHH
MNSIGRTINSALQLSKNGGGVGLNLTNLRAAGAPIKGYDGVAAGVVPVMKLFEDAFSYAN
HHHHHHHHHHHHHCCCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLSLFHPDIMEFLSTKKENADEKIRVKTLSLGVTVPDKFYELVKKGETM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEE
YLFEPYFVEKYYGKPFAEVDITAEYDKMVANPEIRKTPINARELEQELSKLQQESGYPYI
EEECHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
VNVDTVNQANPVDGVISMSNLCSEILQVQTPSVLNDDQTYKVVGTDVACNLGSTNVLNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHC
TSSDEFGTSVESMVRALTFISETSNLDTVPTVRKGNDEMHAIGLGAMGLHSFLAQNQIHY
CCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCC
DSKEAVEFTSVYFMLLNYYTLVASNKLAVEKGSSFKNFEKSAYADGSYFDKYLTHDYFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
VSPKVQELFKGIAVPTIADWTELRDTVKTSGLYNSYRMAVAPNGSISYINDCSSSIHPIV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHCCHHHHHHH
NRIEERQEKKVGKIYYPAAGLATETIPYYKSAYDTDMRAVIDVYAAATEHVDQGLSLTLF
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEE
MRSTLPEGLYEWKVANNKMTTRDLSILRNYAYKKGIKTIYYIRTFTDDQEEVGANQCESC
EHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCHHHHHC
VI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]