Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 125624145

GI number: 125624145

Start: 1301096

End: 1302163

Strand: Reverse

Name: potD [H]

Synonym: llmg_1325

Alternate gene names: 125624145

Gene position: 1302163-1301096 (Counterclockwise)

Preceding gene: 125624146

Following gene: 125624143

Centisome position: 51.48

GC content: 34.36

Gene sequence:

>1068_bases
TTGAAAAAATTATTATACTTTTTTGTTGGAATTCTGGTCATTACTGCTTCACTAGGACTGATTACCAATAGAATGAAAGC
TGCTGACGGAGTCAAAACTCAGAATAATAGCTTGACAATTTTTAACTGGGGTGAATATATCGATCCAGACTTGATTAGTA
AATTTCAAAAAGAAACAGGTTATAAAGTTAACTATATGACTTTTGATTCTAATGAAGCCATGTATTCAAAAGTTAAACAA
GGAGGAACAGCTTACGACCTGATTGTTCCTTCTGATTACATGATTGAAAAAATGGCCAAAGAAAATCTGCTCCTTAAGCT
AGACCACAGCAAAATTAAAGGTTTAAAAGACGATGACCCTAAACTACTTAATCCAGCTTTCGACCAAGGAAATCAATATT
CTGTTCCATACTTTTGGGGAACATTAGGCATAGTTTATAACTCAAAAACAGTAAAAACTCCGCCAAAAACGTGGAATGAT
ATCTGGTCACCAACTTACAAAAACTCGATTTTACTGACAGACAGTGTGCGCGACGTCATGGCAATGGTTCTTAGTAAACA
TGGCTATTCATTAAATACAACCAACCAAAAAGAGTTAGACATAGCCTATCAAGACCTCCTTAAGCTTACGCCAAATGTAA
AAGCAATTCTGGGTGATGAAATCATGAATTACATGATTAATAATGAGACACCTTTAGCCGTTGTTTATAGCGGACAAGCG
GCAGAGATGACCTCTGAAAATAAAAATTTGAAATATGTCATCCCTGAACGTACAAATATTTGGTATGATAATTTAGCTAT
TCCGAAAACAGCAAAAAATGTCAAAGCAGCTTACGCTTTTATTAATTTCATGCAGGAACCCAAAAATGCAGCTCAAAATG
CAGAGTGGATTGGTTATGCAACTCCGAACTTGAAAGCAAAAGCTCTCTTACCCAAAGAAATTCGTGATAATAAATCGTTT
TACCCAGATGAAGCAACTATCAAAGATGATGAAGCTTACAAAAACCTCAATCCATATTGGACTGGAATTTATAATGATTT
GTATTTAGAATTTAAGATGAATAAATAA

Upstream 100 bases:

>100_bases
ATTATATTATGAAAGATTCAGAAACCGTTGGAAAATCTTCAAATCTGACCTAAATAGTTCTTATTGATACTTTGTAGTTC
AAAGTATAGAAAGGAGAAAC

Downstream 100 bases:

>100_bases
TTGGAGAGAGTTTGCCTAATTTGAATGTGTCTGCAGAACTAGTAAAACCACTAAAATTATCAAGTCTATAATACAGTTAA
GTGCAAAATTAAAAATAAAT

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKKLLYFFVGILVITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLLKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVKTPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQA
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAEWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK

Sequences:

>Translated_355_residues
MKKLLYFFVGILVITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLLKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVKTPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQA
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAEWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
>Mature_355_residues
MKKLLYFFVGILVITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLLKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVKTPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQA
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAEWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=43.8650306748466, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1787078, Length=342, Percent_Identity=32.4561403508772, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40573; Mature: 40573

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLYFFVGILVITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCC
YKVNYMTFDSNEAMYSKVKQGGTAYDLIVPSDYMIEKMAKENLLLKLDHSKIKGLKDDDP
CEEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECHHHHCCCCCCCC
KLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVKTPPKTWNDIWSPTYKNSILLTDSVRDVM
CCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHH
AMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQA
HHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCH
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAEWIGYA
HHHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TPNLKAKALLPKEIRDNKSFYPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
CCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECC
>Mature Secondary Structure
MKKLLYFFVGILVITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCC
YKVNYMTFDSNEAMYSKVKQGGTAYDLIVPSDYMIEKMAKENLLLKLDHSKIKGLKDDDP
CEEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECHHHHCCCCCCCC
KLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVKTPPKTWNDIWSPTYKNSILLTDSVRDVM
CCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHH
AMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQA
HHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCH
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAEWIGYA
HHHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TPNLKAKALLPKEIRDNKSFYPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
CCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]