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The map label for this gene is hisH [H]

Identifier: 125624114

GI number: 125624114

Start: 1262312

End: 1262920

Strand: Reverse

Name: hisH [H]

Synonym: llmg_1292

Alternate gene names: 125624114

Gene position: 1262920-1262312 (Counterclockwise)

Preceding gene: 125624115

Following gene: 125624113

Centisome position: 49.93

GC content: 37.11

Gene sequence:

>609_bases
ATGGCAAATATTGTTATCATCGACTACAACCTTGGAAATCTTCAAAGTGTCCAAGCAGCCTTTTTACGATTGGGACAAGA
AACAGTAATTTCGAGAGATTTAGAAGAAATTCGCAGGGCAGATGCACTTATTCTTCCAGGAGTGGGGGCTTTTCCTACCG
CAATGAATAACCTGAAAAAGTTTAATTTAATTGATGTTTTAAAGGAGCGAGCTGATGCCGGAATACCAATATTAGGGATT
TGTCTAGGCATGCAAGTCCTTTTTGAAAAAGGAATGGAAATAAAGGAATGCCAAGGGTTAGGACTTTTAAAAGGGGAAGT
CATTCCAATAAAATCAAGTGAGAAAATTCCACACATGGGTTGGAATCAATTAAAACTAGTCAAAACTCAGCCAATAACTG
CTTTTTTGGCTGATAGTGATGAAGTTTATTTTGTTCACTCCTATCAAGCGACTTGTCCTGATGATGAACTCATTGCCTAT
ACAACCTATGGAGAAGCAATAATCCCTGCCATTGTGGGAAAAAATAACATTATTGGTTGTCAATTTCACCCAGAAAAAAG
TGGAGAAATTGGCAGAAAAATACTAAGAGCGTTTTTGGAGGAAATTTAA

Upstream 100 bases:

>100_bases
TTAATATTGACCGATTGAAATTAATTGGCTGTTGTGAGGTTTTTGGATAATTTTAAATATAAATAGGAATTTATTTCCAT
TTACTATTGAAAGGAGAAGT

Downstream 100 bases:

>100_bases
TGGAAATTATCCCAGCGATTGATTTACAAAATGGAGAAGCTGTGCGTCTCTACAAGGGGGATTATGATAAGAAAACGGTC
TACTCTAAAAATCCACTAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MANIVIIDYNLGNLQSVQAAFLRLGQETVISRDLEEIRRADALILPGVGAFPTAMNNLKKFNLIDVLKERADAGIPILGI
CLGMQVLFEKGMEIKECQGLGLLKGEVIPIKSSEKIPHMGWNQLKLVKTQPITAFLADSDEVYFVHSYQATCPDDELIAY
TTYGEAIIPAIVGKNNIIGCQFHPEKSGEIGRKILRAFLEEI

Sequences:

>Translated_202_residues
MANIVIIDYNLGNLQSVQAAFLRLGQETVISRDLEEIRRADALILPGVGAFPTAMNNLKKFNLIDVLKERADAGIPILGI
CLGMQVLFEKGMEIKECQGLGLLKGEVIPIKSSEKIPHMGWNQLKLVKTQPITAFLADSDEVYFVHSYQATCPDDELIAY
TTYGEAIIPAIVGKNNIIGCQFHPEKSGEIGRKILRAFLEEI
>Mature_201_residues
ANIVIIDYNLGNLQSVQAAFLRLGQETVISRDLEEIRRADALILPGVGAFPTAMNNLKKFNLIDVLKERADAGIPILGIC
LGMQVLFEKGMEIKECQGLGLLKGEVIPIKSSEKIPHMGWNQLKLVKTQPITAFLADSDEVYFVHSYQATCPDDELIAYT
TYGEAIIPAIVGKNNIIGCQFHPEKSGEIGRKILRAFLEEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=39.6984924623116, Blast_Score=130, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=33.8028169014084, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22313; Mature: 22182

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIVIIDYNLGNLQSVQAAFLRLGQETVISRDLEEIRRADALILPGVGAFPTAMNNLKK
CCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
FNLIDVLKERADAGIPILGICLGMQVLFEKGMEIKECQGLGLLKGEVIPIKSSEKIPHMG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCEECCCEEECCCCCCCCCCC
WNQLKLVKTQPITAFLADSDEVYFVHSYQATCPDDELIAYTTYGEAIIPAIVGKNNIIGC
CCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCCCEEEEEECCCHHHHHHCCCCCEEEE
QFHPEKSGEIGRKILRAFLEEI
EECCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
ANIVIIDYNLGNLQSVQAAFLRLGQETVISRDLEEIRRADALILPGVGAFPTAMNNLKK
CEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
FNLIDVLKERADAGIPILGICLGMQVLFEKGMEIKECQGLGLLKGEVIPIKSSEKIPHMG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCEECCCEEECCCCCCCCCCC
WNQLKLVKTQPITAFLADSDEVYFVHSYQATCPDDELIAYTTYGEAIIPAIVGKNNIIGC
CCCEEEEEECCEEEEEECCCCEEEEEECCCCCCCCCEEEEEECCCHHHHHHCCCCCEEEE
QFHPEKSGEIGRKILRAFLEEI
EECCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400209; 11337471 [H]