Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 125624112

GI number: 125624112

Start: 1260824

End: 1261603

Strand: Reverse

Name: hisF

Synonym: llmg_1290

Alternate gene names: 125624112

Gene position: 1261603-1260824 (Counterclockwise)

Preceding gene: 125624113

Following gene: 125624111

Centisome position: 49.88

GC content: 36.67

Gene sequence:

>780_bases
ATGCTAACAAAAAGAATTATCCCTTGTCTTGATATTAAAAATGGTAAGGTCGTTAAGGGAATCAATTTTGTTGGTCTAAA
AGAAATTGGTGACCCAGTGGAGCTAGCAAAAATATATGAAGAACAATGTGCAGATGAAATTGTTTTTCTTGATATAACGG
CTTCTTTTGAAGAACGTGAAATTATTGGTGAGTTAATCGGACGTGCAGCACGTGAGCTTTCAATTCCATTAACCGTTGGT
GGAGGAATTCGTTCTATTAACGATTTTAGAAGAATTCTTGCTAGTGGAGCTGACAAAGTGTCCATAAATTCGGCGGCAAT
AGAAAATCCTGAATTTATCCATCAAGCGGCTAATGAATTTGGCGTTCAATGCGTTGTGGTTGCGATTGATGCCAAGGCGA
ATGAATCGGGGTCTAGCTTTGAAGTTTATATCAAAGGTGGGCGAGAAAATGCAGGAATTGATTTAGTTGAATGGGCCAAA
AAATGTGAAAAATTAGGTGCAGGGGAGATTTTATTGACCTCAATGGATAAAGATGGTACAAAAACAGGCTATGATTTAAA
AATGTTAAATGCCGTTTGCTCTGCAGTTAATATTCCCGTTGTTGCAAGTGGTGGTTGTGGTTCTATTTCGGATATTATTG
ATGTTTTTGAAAAGACAAAAAGTGATGCGGCCCTTGTTGCTTCTTTATTTCATTATGGAGAAGCAACAGTGGATGAAGTT
AAAGAAGAACTCATAAAAAATCGTATTCCAGCAAGAATAATTAAGAAGGAGATTATATGA

Upstream 100 bases:

>100_bases
AGTTCGGATATTCAACGAGCAGCAGATTCTGATTTCTATGGAATTATTGTTGGAAAAGCTTACTATGAAGGAAAAATTAA
TCTTGAAAAGGAGTTCAATC

Downstream 100 bases:

>100_bases
AACCAGATTTTCAAAAGCAGGCGCTCATTCCTGTCATTGTGCAAGATTATCAAACGAAGCAAGTTTTGATGATGGCTTAT
ACAAACGAGGAGGCCTATCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLTKRIIPCLDIKNGKVVKGINFVGLKEIGDPVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTVG
GGIRSINDFRRILASGADKVSINSAAIENPEFIHQAANEFGVQCVVVAIDAKANESGSSFEVYIKGGRENAGIDLVEWAK
KCEKLGAGEILLTSMDKDGTKTGYDLKMLNAVCSAVNIPVVASGGCGSISDIIDVFEKTKSDAALVASLFHYGEATVDEV
KEELIKNRIPARIIKKEII

Sequences:

>Translated_259_residues
MLTKRIIPCLDIKNGKVVKGINFVGLKEIGDPVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTVG
GGIRSINDFRRILASGADKVSINSAAIENPEFIHQAANEFGVQCVVVAIDAKANESGSSFEVYIKGGRENAGIDLVEWAK
KCEKLGAGEILLTSMDKDGTKTGYDLKMLNAVCSAVNIPVVASGGCGSISDIIDVFEKTKSDAALVASLFHYGEATVDEV
KEELIKNRIPARIIKKEII
>Mature_259_residues
MLTKRIIPCLDIKNGKVVKGINFVGLKEIGDPVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTVG
GGIRSINDFRRILASGADKVSINSAAIENPEFIHQAANEFGVQCVVVAIDAKANESGSSFEVYIKGGRENAGIDLVEWAK
KCEKLGAGEILLTSMDKDGTKTGYDLKMLNAVCSAVNIPVVASGGCGSISDIIDVFEKTKSDAALVASLFHYGEATVDEV
KEELIKNRIPARIIKKEII

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.0233463035019, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=28.4518828451883, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.5555555555556, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_LACLM (A2RKR7)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032595.1
- ProteinModelPortal:   A2RKR7
- SMR:   A2RKR7
- STRING:   A2RKR7
- GeneID:   4797606
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1290
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27930; Mature: 27930

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDIKNGKVVKGINFVGLKEIGDPVELAKIYEEQCADEIVFLDITASFEERE
CCCCCCCCCEECCCCCEEECCCEEEHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHH
IIGELIGRAARELSIPLTVGGGIRSINDFRRILASGADKVSINSAAIENPEFIHQAANEF
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GVQCVVVAIDAKANESGSSFEVYIKGGRENAGIDLVEWAKKCEKLGAGEILLTSMDKDGT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KTGYDLKMLNAVCSAVNIPVVASGGCGSISDIIDVFEKTKSDAALVASLFHYGEATVDEV
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
KEELIKNRIPARIIKKEII
HHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLTKRIIPCLDIKNGKVVKGINFVGLKEIGDPVELAKIYEEQCADEIVFLDITASFEERE
CCCCCCCCCEECCCCCEEECCCEEEHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHH
IIGELIGRAARELSIPLTVGGGIRSINDFRRILASGADKVSINSAAIENPEFIHQAANEF
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GVQCVVVAIDAKANESGSSFEVYIKGGRENAGIDLVEWAKKCEKLGAGEILLTSMDKDGT
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KTGYDLKMLNAVCSAVNIPVVASGGCGSISDIIDVFEKTKSDAALVASLFHYGEATVDEV
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
KEELIKNRIPARIIKKEII
HHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA