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The map label for this gene is leuC [H]

Identifier: 125624108

GI number: 125624108

Start: 1253875

End: 1255251

Strand: Reverse

Name: leuC [H]

Synonym: llmg_1284

Alternate gene names: 125624108

Gene position: 1255251-1253875 (Counterclockwise)

Preceding gene: 125624110

Following gene: 125624107

Centisome position: 49.62

GC content: 42.19

Gene sequence:

>1377_bases
ATGTCAGGTCAAACTATATTTGATAAACTTTGGAACCAGCATGTGATTGCTGGACAGGAGGGTGAACCTCAACTTTTGTA
TATTGATCTTCATGTTATACACGAAGTAACGAGTCCACAGGCATTTCAAGGCTTGCGTGATGCAGGGCGTAGCGTTCGAC
GCAGGGATTTAACTTATGGAACACTTGACCATAATGTTCCAACAAAAGATATTTTTAATATTCAAGATTTGATTTCTAAA
AAGCAAATTGATACCTTTACTAAAAATGTCAAGGAATTTGGTATACCGGCAGAAGCACATGGCGGCAAAGGACAAGGAAT
TGTTCACATGGTCGCCCCTGAATCTGGCAGAACTCAACCGGGGAAAATAATTGTTTGTGGCGATAGTCATACCGCAACAA
ATGGGGCTTTTGGCGCTATTGCGTTCGGAATTGGAACCAGTGAAGTAGAGCATGTCCTTGCGACTCAAACCATCTGGCAA
GTCAAACCTAAACGTATGAAAATTGAATTTCAAGGTCATCCTCAAAAAGGAGTTTATAGTAAAGATTTTATTCTCGCTCT
CATTGCCAAATATGGGGTTGATGCAGGTGTAGGTTATGCGGTTGAGTATACTGGGAATGCCATTAGTGATTTAAGCATGG
AAGAACGGATGACAATTTGTAACATGTCCATTGAATTTGGGGCAAAAATGGGCCTGATGAATCCTGACGAAAAAACTTAT
GATTATGTCAAAGGACGCGAATATGCTCCTAAAGATTTTGACGAAGCTGTCAGTAAATGGGAAAAACTTGTCAGTGATTC
TGACGCGGTTTATGACAAGGTCTTAACCCTTGATGTTAGTCAATTAAAACCCATGGTGACTTGGGGAACCAATCCGGGCA
TGGGACTAGAATTTGGTGAAGAATTTCCTAAAATTAATGACGACTTGAACTACGAACGTGCTTATCAGTATATGGATTTA
AAACCGGGACAAACCGCTTCTGATATTGACTTGGGTTATATTTTCATCGGTTCTTGTACCAATGCACGGCTTGGCGATTT
AGAAGAAGCAGCAAAAATTATCAAAGGCAATCATATTGCTGACGGACTGACAGGAATTGTTGTACCCGGCAGTCGCCCTG
TCAAAAGGGCAGCCGAAGAACTGGGTCTTGATAAAATCTTCAAGGACGCAGGTTTTGAATGGCGTGAACCCGGATGTTCG
GCTTGTCTGGGCATGAATCCTGACCAAATCCCAGCTTATGTCCATTGCGCATCTACAAGTAATCGAAACTTTGAGGGACG
TCAAGGACATAATGCCAGAACGCATTTATGCTCTCCAGCAATGGCGGCCGCAGCCGCAATCTCCGGCAAATTTGTTGATG
TTAGAGAAATTGTCTAA

Upstream 100 bases:

>100_bases
TCAGCAATGCTGAGAGTTCTGTCAGTGAATTTTACTGACGAAAAGTTAAAAAATACTGACAGCTCTGTCAGTAAAATTTT
ATAAAGAAAAGAGATAAAAA

Downstream 100 bases:

>100_bases
TGAATACGAACCAATTTAATCAGGTGCTCAACACTGACATTCAAGCTTTCATTCAGAACTTTGCACCAGCGATTTTGGGG
ATTCTCATCATCTTCTGGGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYGTLDHNVPTKDIFNIQDLISK
KQIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQPGKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQ
VKPKRMKIEFQGHPQKGVYSKDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTY
DYVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGEEFPKINDDLNYERAYQYMDL
KPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIADGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCS
ACLGMNPDQIPAYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV

Sequences:

>Translated_458_residues
MSGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYGTLDHNVPTKDIFNIQDLISK
KQIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQPGKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQ
VKPKRMKIEFQGHPQKGVYSKDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTY
DYVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGEEFPKINDDLNYERAYQYMDL
KPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIADGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCS
ACLGMNPDQIPAYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV
>Mature_457_residues
SGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYGTLDHNVPTKDIFNIQDLISKK
QIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQPGKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQV
KPKRMKIEFQGHPQKGVYSKDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTYD
YVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGEEFPKINDDLNYERAYQYMDLK
PGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIADGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCSA
CLGMNPDQIPAYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=360, Percent_Identity=28.8888888888889, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI8659555, Length=394, Percent_Identity=26.3959390862944, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI41352693, Length=365, Percent_Identity=24.9315068493151, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=54.7413793103448, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.4847645429363, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081781, Length=359, Percent_Identity=23.9554317548747, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=361, Percent_Identity=30.1939058171745, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=361, Percent_Identity=30.1939058171745, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=460, Percent_Identity=27.6086956521739, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=30.0653594771242, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=50.8510638297872, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.0193236714976, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=365, Percent_Identity=30.6849315068493, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=28.8828337874659, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=27.2264631043257, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=27.2264631043257, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=27.2264631043257, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137564, Length=456, Percent_Identity=26.3157894736842, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645686, Length=454, Percent_Identity=26.431718061674, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50245; Mature: 50113

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYG
CCCCHHHHHHCCCEEEECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCEEE
TLDHNVPTKDIFNIQDLISKKQIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
GKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGVYS
CEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
KDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTY
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHEEEECCHHHCCEECCCCCCHHHH
DYVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGE
HHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHCCCEEEECCCCCCCCCHHH
EFPKINDDLNYERAYQYMDLKPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIA
HCCCCCCCCCHHHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
DGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCSACLGMNPDQIPAYVHCASTS
CCCCEEEECCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEEEECCC
NRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV
CCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEHHHCC
>Mature Secondary Structure 
SGQTIFDKLWNQHVIAGQEGEPQLLYIDLHVIHEVTSPQAFQGLRDAGRSVRRRDLTYG
CCCHHHHHHCCCEEEECCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCEEE
TLDHNVPTKDIFNIQDLISKKQIDTFTKNVKEFGIPAEAHGGKGQGIVHMVAPESGRTQP
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCC
GKIIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGVYS
CEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
KDFILALIAKYGVDAGVGYAVEYTGNAISDLSMEERMTICNMSIEFGAKMGLMNPDEKTY
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHEEEECCHHHCCEECCCCCCHHHH
DYVKGREYAPKDFDEAVSKWEKLVSDSDAVYDKVLTLDVSQLKPMVTWGTNPGMGLEFGE
HHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHCCCEEEECCCCCCCCCHHH
EFPKINDDLNYERAYQYMDLKPGQTASDIDLGYIFIGSCTNARLGDLEEAAKIIKGNHIA
HCCCCCCCCCHHHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
DGLTGIVVPGSRPVKRAAEELGLDKIFKDAGFEWREPGCSACLGMNPDQIPAYVHCASTS
CCCCEEEECCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEEEECCC
NRNFEGRQGHNARTHLCSPAMAAAAAISGKFVDVREIV
CCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]