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The map label for this gene is leuD

Identifier: 125624106

GI number: 125624106

Start: 1252718

End: 1253293

Strand: Reverse

Name: leuD

Synonym: llmg_1282

Alternate gene names: 125624106

Gene position: 1253293-1252718 (Counterclockwise)

Preceding gene: 125624107

Following gene: 125624105

Centisome position: 49.55

GC content: 34.72

Gene sequence:

>576_bases
ATGGAAAAATTCACGATTTACAAAGGAACAAGCGTTCCATTAATGAACGACAATATTGACACTGACCAAATTATTCCTAA
ACAATTTTTAAAAGCGATTGATAAAAAAGGTTTTGGGAAAAACTTATTTTATGAATGGCGCTATAGCAAAGATTATGAGG
AAAATCCCGATTTTATGTTAAATAAGCCGCAATATCGTAAGGCCAGCTTGTTAATTTCTGGCGATAATTTTGGCTCAGGA
TCTTCCAGAGAACATGCGGCTTGGGCATTAGCAGATTATGGTTTTCGAGCCATTATTGCAGGTTCTTACTCAGACATTTT
TTATAATAACAGCTTGAAAAATGGACTTTTACCCATTGTCCAACCCAAAGAGGCGCTAAAAAGTCTAGCCCAACTTTCAA
GCCAAGAAGAAATCACAATTGATTTGCCTCATCAGTTAATTCAAACGAGCACAGAAAACTTTTATTTTGAGATTGACCCC
ATTTGGAAAGATAAACTGATTAATGGTTTAGATGATATTGGGATTACTTTGCAATATGAACAAGCTATTACTGCCTATGA
GCAAAAACATCAATAA

Upstream 100 bases:

>100_bases
GCGCAAGCAATTTCCAAAAGAAATTGAGAATAATCGAAAAGCAAAAGAATGAAAATCAACAGACAGTTATTTAAATATAA
AAAATAATAGAAAGATAATA

Downstream 100 bases:

>100_bases
AAGAGAGAAAAAGATGACAATTATTAGCTTAAAAAATGTGAATCTTACTCGAAATAAAACAGAAATTCTCAAAAATATCA
CTTGGCAGGTTAATCAAGGG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEKFTIYKGTSVPLMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYSKDYEENPDFMLNKPQYRKASLLISGDNFGSG
SSREHAAWALADYGFRAIIAGSYSDIFYNNSLKNGLLPIVQPKEALKSLAQLSSQEEITIDLPHQLIQTSTENFYFEIDP
IWKDKLINGLDDIGITLQYEQAITAYEQKHQ

Sequences:

>Translated_191_residues
MEKFTIYKGTSVPLMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYSKDYEENPDFMLNKPQYRKASLLISGDNFGSG
SSREHAAWALADYGFRAIIAGSYSDIFYNNSLKNGLLPIVQPKEALKSLAQLSSQEEITIDLPHQLIQTSTENFYFEIDP
IWKDKLINGLDDIGITLQYEQAITAYEQKHQ
>Mature_191_residues
MEKFTIYKGTSVPLMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYSKDYEENPDFMLNKPQYRKASLLISGDNFGSG
SSREHAAWALADYGFRAIIAGSYSDIFYNNSLKNGLLPIVQPKEALKSLAQLSSQEEITIDLPHQLIQTSTENFYFEIDP
IWKDKLINGLDDIGITLQYEQAITAYEQKHQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=47.1502590673575, Blast_Score=184, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=51.530612244898, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_LACLM (A2RKR1)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032589.1
- ProteinModelPortal:   A2RKR1
- SMR:   A2RKR1
- STRING:   A2RKR1
- GeneID:   4799063
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1282
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21948; Mature: 21948

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTIYKGTSVPLMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYSKDYEENPDFML
CCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEE
NKPQYRKASLLISGDNFGSGSSREHAAWALADYGFRAIIAGSYSDIFYNNSLKNGLLPIV
CCCCCCEEEEEEECCCCCCCCCCCHHEEEHHHCCEEEEEECCCCCEEECCCCCCCCCCCC
QPKEALKSLAQLSSQEEITIDLPHQLIQTSTENFYFEIDPIWKDKLINGLDDIGITLQYE
CCHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEEEHH
QAITAYEQKHQ
HHHHHHHHCCC
>Mature Secondary Structure
MEKFTIYKGTSVPLMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYSKDYEENPDFML
CCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEE
NKPQYRKASLLISGDNFGSGSSREHAAWALADYGFRAIIAGSYSDIFYNNSLKNGLLPIV
CCCCCCEEEEEEECCCCCCCCCCCHHEEEHHHCCEEEEEECCCCCEEECCCCCCCCCCCC
QPKEALKSLAQLSSQEEITIDLPHQLIQTSTENFYFEIDPIWKDKLINGLDDIGITLQYE
CCHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCEEEEHH
QAITAYEQKHQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA