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The map label for this gene is pyk [H]

Identifier: 125623950

GI number: 125623950

Start: 1079292

End: 1080800

Strand: Direct

Name: pyk [H]

Synonym: llmg_1119

Alternate gene names: 125623950

Gene position: 1079292-1080800 (Clockwise)

Preceding gene: 125623949

Following gene: 125623951

Centisome position: 42.67

GC content: 40.69

Gene sequence:

>1509_bases
ATGAATAAACGTGTAAAAATCGTCTCAACTCTTGGACCTGCTGTCGAAATTCGTGGTGGCAAAAAATTTGGTGAAAGCGG
CTATTGGGGAGAATCTCTTGACGTCGAAGCATCAGCAAAAAACATTGCAGCTTTGATTGAAGAAGGCGCTAATGTCTTCC
GTTTCAACTTCTCACACGGTGACCATCCTGAACAAGGTGCTCGTATGGCAACTGTTCACCGCGCTGAAGAAATCGCTGGT
CACAAAGTAGGTTTCTTGCTTGATACTAAAGGTCCTGAAATGCGTACAGAACTCTTCACTGACGGTGCTGATTCAATCAG
CGTTGTTACTGGAGATAAATTCCGTGTTGCAACTAAACAAGGTCTTAAATCAACTCCTGAATTGATTGCCTTGAACGTAG
CTGGCGGACTTGACATCTTTGATGATGTTGAAATCGGTCAAACTATCTTGATTGATGATGGTAAACTTGGTTTGTCATTG
ACTGGTAAAGATGCAGCAACTCGTGAATTCGAAGTTGAAGCACAAAATGATGGTGTTATCGGTAAACAAAAAGGTGTTAA
CATTCCTAACACTAAAATTCCTTTCCCAGCCCTTGCTGAACGTGACGATGCAGATATCCGTTTCGGTCTTTCACAACCTG
GTGGAATTAACTTTATCGCTATCTCATTCGTACGTACTGCAAATGACGTTAAAGAAGTACGTCGTATTTGTGAAGAAACT
GGTAATCCACACGTACAACTTTTGGCTAAAATTGAAAACCAACAAGGGATTGAAAACCTTGATGAAATCATCGAAGCAGC
TGATGGTATCATGATTGCTCGTGGTGACATGGGTATCGAAGTTCCATTTGAAATGGTTCCAGTTTACCAAAAATTGATTA
TCAGCAAAGTTAACAAAGCAGGTAAAATTGTTGTAACTGCAACAAACATGCTTGAATCAATGACTTACAACCCACGTGCT
ACACGTTCAGAAATTTCAGACGTATTTAACGCTGTTATTGACGGAACTGATGCTACAATGCTTTCAGGTGAGTCTGCTAA
TGGTAAATACCCACGCGAATCAGTTCGTACAATGGCTACTGTTAACAAAAATGCTCAAACAATGTTGAAAGAATACGGAC
GTCTTCACCCAGAACGTTACGACAAATCAACTGTAACAGAAGTTGTTGCTGCATCAGTTAAAAATGCTGCAGAAGCTATG
GATGTTAAATTGATTGTTGCTTTGACTGAATCTGGTAACACAGCTCGTTTGATTTCTAAACACCGTCCAGATGCTGATAT
CTTGGCTATCACATTTGACGAAAAAGTTGAACGTGGATTGATGATCAACTGGGGTGTTATCCCAATGATGACTGAAAAAC
CTGCATCTACTGATGACATGTTTGAAGTTGCTGAAAAAGTTGCATTGGCTTCTGGTTTGGTTGAAGCTGGCGACAATATC
ATCATCGTTGCTGGTGTTCCAGTTGGTACTGGTCGCACTAATACAATGCGTATCCGCACTGTTAAATAA

Upstream 100 bases:

>100_bases
TAACCTTAACTAAGTTACTGCAAATCTGTTTCTAGTTAAGTGTTAAACGCATAATTAGGGCAGAGATATATAATTAATCA
TTATAGGAGAAAAACACAAA

Downstream 100 bases:

>100_bases
TTTGTCAATTAATGATCTTAAAACAATACAGCCTGCCGCTATCGACAGGCCCTATTGTTGAAAAGATTATATTTTATAAA
ATACTCGTTTATAATTTGAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFTDGADSISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DVKLIVALTESGNTARLISKHRPDADILAITFDEKVERGLMINWGVIPMMTEKPASTDDMFEVAEKVALASGLVEAGDNI
IIVAGVPVGTGRTNTMRIRTVK

Sequences:

>Translated_502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFTDGADSISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DVKLIVALTESGNTARLISKHRPDADILAITFDEKVERGLMINWGVIPMMTEKPASTDDMFEVAEKVALASGLVEAGDNI
IIVAGVPVGTGRTNTMRIRTVK
>Mature_502_residues
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAG
HKVGFLLDTKGPEMRTELFTDGADSISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSL
TGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRA
TRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAM
DVKLIVALTESGNTARLISKHRPDADILAITFDEKVERGLMINWGVIPMMTEKPASTDDMFEVAEKVALASGLVEAGDNI
IIVAGVPVGTGRTNTMRIRTVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=485, Percent_Identity=37.5257731958763, Blast_Score=298, Evalue=7e-81,
Organism=Homo sapiens, GI33286422, Length=481, Percent_Identity=37.2141372141372, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI33286420, Length=481, Percent_Identity=37.2141372141372, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI10835121, Length=485, Percent_Identity=36.2886597938144, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI32967597, Length=485, Percent_Identity=36.2886597938144, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI310128732, Length=282, Percent_Identity=37.5886524822695, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128730, Length=282, Percent_Identity=37.5886524822695, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128736, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI310128734, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI310128738, Length=223, Percent_Identity=34.0807174887892, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787965, Length=499, Percent_Identity=39.4789579158317, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788160, Length=508, Percent_Identity=32.0866141732283, Blast_Score=212, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17544584, Length=513, Percent_Identity=35.672514619883, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI71984413, Length=512, Percent_Identity=35.15625, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=512, Percent_Identity=35.15625, Blast_Score=274, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=512, Percent_Identity=35.15625, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=510, Percent_Identity=35.2941176470588, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=477, Percent_Identity=37.5262054507338, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=511, Percent_Identity=36.2035225048924, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24648964, Length=515, Percent_Identity=37.4757281553398, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI28571814, Length=515, Percent_Identity=37.4757281553398, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=37.7403846153846, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=32.8482328482328, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24646914, Length=264, Percent_Identity=35.9848484848485, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54287; Mature: 54287

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHG
CCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCC
DHPEQGARMATVHRAEEIAGHKVGFLLDTKGPEMRTELFTDGADSISVVTGDKFRVATKQ
CCCCCCCEEHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCCEEEEHHH
GLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSLTGKDAATREFEVEAQNDGVI
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEEEECCCCCE
GKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHC
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKA
CCCEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
GKIVVTATNMLESMTYNPRATRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMAT
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHH
VNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAMDVKLIVALTESGNTARLISK
CCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEHHHC
HRPDADILAITFDEKVERGLMINWGVIPMMTEKPASTDDMFEVAEKVALASGLVEAGDNI
CCCCCCEEEEEECHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IIVAGVPVGTGRTNTMRIRTVK
EEEEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHG
CCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCC
DHPEQGARMATVHRAEEIAGHKVGFLLDTKGPEMRTELFTDGADSISVVTGDKFRVATKQ
CCCCCCCEEHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCCEEEEHHH
GLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSLTGKDAATREFEVEAQNDGVI
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEEEEEEECCCCCE
GKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEET
ECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHC
GNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKA
CCCEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
GKIVVTATNMLESMTYNPRATRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMAT
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHH
VNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAMDVKLIVALTESGNTARLISK
CCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEHHHC
HRPDADILAITFDEKVERGLMINWGVIPMMTEKPASTDDMFEVAEKVALASGLVEAGDNI
CCCCCCEEEEEECHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IIVAGVPVGTGRTNTMRIRTVK
EEEEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471 [H]