Click here to switch to the map view.

The map label for this gene is busAB [H]

Identifier: 125623880

GI number: 125623880

Start: 1012207

End: 1013928

Strand: Direct

Name: busAB [H]

Synonym: llmg_1049

Alternate gene names: 125623880

Gene position: 1012207-1013928 (Clockwise)

Preceding gene: 125623879

Following gene: 125623881

Centisome position: 40.02

GC content: 36.82

Gene sequence:

>1722_bases
ATGATTGATTTAGTGATTGGAAAAATACCATTAGCCAACTGGGTATCGTCAGCAACAGATTGGATTACAAGTACATTTAG
TTCGGGTTTTGATGTGATTCAAAAATCAGGAACAGTGTTGATGAACGGAATAACAGGTGCTTTAACTGCAGTTCCATTCT
GGCTGATGATTGCTGTTGTTACTATCCTAGCAATTTTGGTTTCAGGTAAAAAGTTTGCCTTCCCATTGTTTGCTTTTATT
GGTCTTTGTTTAATTGCCAATCAAGGCTTATGGTCAGATTTGATGAGTACGATTACCCTTGTCTTACTTTCAAGTTTGTT
ATCAATTATCATAGGGGTCCCTCTAGGAATTTGGATGGCAAAATCTGAACTAGTAGCTAAGATTGTTCAACCTATATTGG
ACTTCATGCAAACCATGCCTGGTTTTGTTTATTTGATTCCAGCAGTTGCCTTCTTTGGTATTGGGGTTGTTCCTGGGGTA
TTTGCTTCAGTAATCTTTGCTTTGCCACCGACTGTCAGAATGACTAACTTAGGTATTCGCCAAGTTTCAACTGAATTAGT
TGAAGCAGCAGATTCATTTGGTTCGACTGCCCGTCAGAAGTTATTTAAATTGGAATTTCCTTTAGCTAAAGGAACAATTA
TGGCTGGTGTTAACCAAACAATTATGTTGGCTCTTTCTATGGTCGTTATTGCCTCTATGATTGGTGCACCTGGTTTAGGT
CGTGGAGTACTAGCTGCTGTTCAATCAGCTGATATTGGTAAGGGATTTGTAAGTGGGATTTCATTAGTTATTTTGGCTAT
TATTATTGACCGCTTTACGCAAAAACTTAATGTTTCTCCTTTAGAAAAACAAGGAAATCCTAAGCTCAAAAAATGGAAAC
GTTGGATTGCTATCGTTTCATTGTTAGCCTTAATTGTTGGTGCTTTTTCGGGAATGTCTTTTGGGAAAAAATCTTCTGAT
AAAAAAGTGGATTTAGTTTATATGAACTGGGACTCAGAAGTTGCGTCAATCAATGTTTTGACCCAAGCAATGGAAGAACA
TAGTTTTGATGTTACAACGACTGCACTTGATAACGCAGTTGCTTGGCAAACTGTGGCTAATAGTCAAGCTGATGGAATGG
TTTCTGCTTGGTTGCCAAACACTCATAAGACCCAATGGAAAAAATATGGTAAGTCTGTTGAGTTATTAGGTCCAAATCTT
AAAGGCGCTAAAGTAGGATTTGTTGTGCCAAGTTATATGAATGTGAATTCAATTGAAGATTTGACTAATCAGGCCAATAA
AACAATCACAGGAATTGAGCCTGGTGCAGGTGTCATGGCTGCCAGTGAAAATACACTTAAGAGCTATAGTAATCTTAAAG
ATTGGAAGCTTGTTCCTTCATCATCTGGTGCAATGACTGTTGCTCTTGGTGAAGCCATTAAACAACATAAAGACATTGTA
ATCACTGGTTGGTCTCCTCATTGGATTTTCAATAAATATGATTTGAAATACTTAGCTGATCCGAAAGGAACAATGGGGAC
TTCAGAAAATATTAATACAATTGTGCGCAAAGGATTGAAAAAAGAAAATCCTGAAGCTTATAAGGTTTTGAATAATTTTA
ATTGGACCACTAAAGATATGGAATCTGTTATGCTTGATATTCAAAATGGTAAGACACCAGAAGCAGCTGCAAAAGCTTGG
ATTAAAGACCATCAAAAACAAGTTGATAAATGGTTTAAATAA

Upstream 100 bases:

>100_bases
CAAGGCTACTTAAAAGGAATTCTGATTCGGGGAATTGTCCTAGAAGCATTGGCAGACATTCCTGATGAAGTAGAAGAAAT
AGAGAAAGAGGAGGAGAAGA

Downstream 100 bases:

>100_bases
TAAGATTATTATTAACTTAAAAAAACTTTCTGACATTATAGTTAGAAGGTTTTTTATTATTTCATTCTAAGTTTATTATT
TTTAGATTTATAACATTTAT

Product: glycine betaine-binding periplasmic protein precursor

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIDLVIGKIPLANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKFAFPLFAFI
GLCLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSELVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPKLKKWKRWIAIVSLLALIVGAFSGMSFGKKSSD
KKVDLVYMNWDSEVASINVLTQAMEEHSFDVTTTALDNAVAWQTVANSQADGMVSAWLPNTHKTQWKKYGKSVELLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASENTLKSYSNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWIFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLNNFNWTTKDMESVMLDIQNGKTPEAAAKAW
IKDHQKQVDKWFK

Sequences:

>Translated_573_residues
MIDLVIGKIPLANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKFAFPLFAFI
GLCLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSELVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPKLKKWKRWIAIVSLLALIVGAFSGMSFGKKSSD
KKVDLVYMNWDSEVASINVLTQAMEEHSFDVTTTALDNAVAWQTVANSQADGMVSAWLPNTHKTQWKKYGKSVELLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASENTLKSYSNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWIFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLNNFNWTTKDMESVMLDIQNGKTPEAAAKAW
IKDHQKQVDKWFK
>Mature_573_residues
MIDLVIGKIPLANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKFAFPLFAFI
GLCLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSELVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPKLKKWKRWIAIVSLLALIVGAFSGMSFGKKSSD
KKVDLVYMNWDSEVASINVLTQAMEEHSFDVTTTALDNAVAWQTVANSQADGMVSAWLPNTHKTQWKKYGKSVELLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASENTLKSYSNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWIFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLNNFNWTTKDMESVMLDIQNGKTPEAAAKAW
IKDHQKQVDKWFK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG2113

COG function: function code E; ABC-type proline/glycine betaine transport systems, periplasmic components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=287, Percent_Identity=47.0383275261324, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=28.8557213930348, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788449, Length=182, Percent_Identity=29.1208791208791, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786564, Length=161, Percent_Identity=29.1925465838509, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 62129; Mature: 62129

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLVIGKIPLANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
TILAILVSGKKFAFPLFAFIGLCLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSELVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGVFASVIFALPPTVRMTNLGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCHHH
QVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPKLKKWKRWIAIVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LLALIVGAFSGMSFGKKSSDKKVDLVYMNWDSEVASINVLTQAMEEHSFDVTTTALDNAV
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHCHH
AWQTVANSQADGMVSAWLPNTHKTQWKKYGKSVELLGPNLKGAKVGFVVPSYMNVNSIED
HHHHHHCCCCCCCEEECCCCCCHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHH
LTNQANKTITGIEPGAGVMAASENTLKSYSNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
HHHHHCCEECCCCCCCCEEECCCHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHCCCEE
ITGWSPHWIFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLNNFNWTTKDM
EECCCCCEEEECCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHH
ESVMLDIQNGKTPEAAAKAWIKDHQKQVDKWFK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDLVIGKIPLANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVV
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
TILAILVSGKKFAFPLFAFIGLCLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSELVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGVFASVIFALPPTVRMTNLGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCHHH
QVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPKLKKWKRWIAIVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LLALIVGAFSGMSFGKKSSDKKVDLVYMNWDSEVASINVLTQAMEEHSFDVTTTALDNAV
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEHHHHHCHH
AWQTVANSQADGMVSAWLPNTHKTQWKKYGKSVELLGPNLKGAKVGFVVPSYMNVNSIED
HHHHHHCCCCCCCEEECCCCCCHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCCHHHHH
LTNQANKTITGIEPGAGVMAASENTLKSYSNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
HHHHHCCEECCCCCCCCEEECCCHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHCCCEE
ITGWSPHWIFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLNNFNWTTKDM
EECCCCCEEEECCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHH
ESVMLDIQNGKTPEAAAKAWIKDHQKQVDKWFK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]