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The map label for this gene is dfrA [H]

Identifier: 125623829

GI number: 125623829

Start: 956790

End: 957827

Strand: Direct

Name: dfrA [H]

Synonym: llmg_0992

Alternate gene names: 125623829

Gene position: 956790-957827 (Clockwise)

Preceding gene: 125623827

Following gene: 125623830

Centisome position: 37.83

GC content: 39.02

Gene sequence:

>1038_bases
ATGACAAATACAAATAAAAAATTAATACTAGTAACTGGTGGTTCGGGTTATATTGCTATGTTCATCATGATTGCTTTATT
AAAAAAAGGCTATCGAGTACGGGCGACCTTGCGGATGATGTCTAGACAAGAAGAAGTGAAGAAAATGATGAAGCAAGGAG
GGATTGCTGATTTTACTGACCTAGACTTTGTTCAAACGGATTTAACTAAAGAAGAAGGATGGAGTCAAGCAATGACTGGA
GTGGATTCTGTCATCCACGTCGCTTCTCCAACACCCTTGCAACGTCCTGACGCTGATGATTTAATGGTAATAATGGCTGT
AGATGGAGTGAAGTTTGTGATGAGAGCAGCCAAGGAAGCAGGAGTTAAACGAGTGGTTTTGACCTCAGCATCTGGTGCTG
TTTTAGCGGGGCATAAGCATCATCCAGAAATCTTTACAGAAAAAGACTGGACAAAGCTTGATGCTCCAATTAATGCTTAT
CAACGTTCTAAAACAATGGCTGAACTGGAGTTTTGGAAACTTGCCAAATCTTATGGTATGGAAGGAGCCTCAGTTCTACC
TACGGCTGTCATGGGACCGATTCTTGGGAATGATTTTTCACATTCTAGCGCGTCAATTAAAAATATGTTTGAAGGAAAAA
TGCCTAGACTTTTAAATTTGGCATTTGACTATGTTGATGTTCGTGATGTTGCTGATTTACATTTGTTATCTCTTGAAAAA
GAAGAAGCCATTAGTCAGCGTTTTATCGCGACTTCAGGGCAAAATATTACTTATCAGGAACAAGCAAAATTTTTGAAAAA
AGAATTTGGTTCAAAAGCCAAGAAAGTTTCTACTCAAGTTATTCCAGACTTTTTAGTGAAGATCTTGGCACGTTTTGATA
AAAGTTTGGCGATGCCAGCCACATTTTTGGGGCAAAATACGGCATGTTCAAATGAGAAATCAACAAGACTTTTAGGTTGG
CAACCCCGTTCGGGAGAAGCAGCCATTATTCAAACAGCGCAAACGATGTTAGATTTGGCTGTGATTAAAGTTGATTAA

Upstream 100 bases:

>100_bases
GCGGATTTTAAGCTTGTATTTTTACTACTTCTATCGTACAATAATTCCACATGAGTTTCAAGTGTAACGGATGTTGTTGA
GAGAGAAAAAGGAGAGAAAA

Downstream 100 bases:

>100_bases
GAAAATATGAATTATAAATTTTACTATTTCCGAACGTTGTAAGCTAAAAAAGACCAAATCTCTGCCTTTACCCTTTTGAT
TCTCTGAAAAAAAGTCTAAA

Product: putative nucleoside-diphosphate-sugar epimerases

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTNTNKKLILVTGGSGYIAMFIMIALLKKGYRVRATLRMMSRQEEVKKMMKQGGIADFTDLDFVQTDLTKEEGWSQAMTG
VDSVIHVASPTPLQRPDADDLMVIMAVDGVKFVMRAAKEAGVKRVVLTSASGAVLAGHKHHPEIFTEKDWTKLDAPINAY
QRSKTMAELEFWKLAKSYGMEGASVLPTAVMGPILGNDFSHSSASIKNMFEGKMPRLLNLAFDYVDVRDVADLHLLSLEK
EEAISQRFIATSGQNITYQEQAKFLKKEFGSKAKKVSTQVIPDFLVKILARFDKSLAMPATFLGQNTACSNEKSTRLLGW
QPRSGEAAIIQTAQTMLDLAVIKVD

Sequences:

>Translated_345_residues
MTNTNKKLILVTGGSGYIAMFIMIALLKKGYRVRATLRMMSRQEEVKKMMKQGGIADFTDLDFVQTDLTKEEGWSQAMTG
VDSVIHVASPTPLQRPDADDLMVIMAVDGVKFVMRAAKEAGVKRVVLTSASGAVLAGHKHHPEIFTEKDWTKLDAPINAY
QRSKTMAELEFWKLAKSYGMEGASVLPTAVMGPILGNDFSHSSASIKNMFEGKMPRLLNLAFDYVDVRDVADLHLLSLEK
EEAISQRFIATSGQNITYQEQAKFLKKEFGSKAKKVSTQVIPDFLVKILARFDKSLAMPATFLGQNTACSNEKSTRLLGW
QPRSGEAAIIQTAQTMLDLAVIKVD
>Mature_344_residues
TNTNKKLILVTGGSGYIAMFIMIALLKKGYRVRATLRMMSRQEEVKKMMKQGGIADFTDLDFVQTDLTKEEGWSQAMTGV
DSVIHVASPTPLQRPDADDLMVIMAVDGVKFVMRAAKEAGVKRVVLTSASGAVLAGHKHHPEIFTEKDWTKLDAPINAYQ
RSKTMAELEFWKLAKSYGMEGASVLPTAVMGPILGNDFSHSSASIKNMFEGKMPRLLNLAFDYVDVRDVADLHLLSLEKE
EAISQRFIATSGQNITYQEQAKFLKKEFGSKAKKVSTQVIPDFLVKILARFDKSLAMPATFLGQNTACSNEKSTRLLGWQ
PRSGEAAIIQTAQTMLDLAVIKVD

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=347, Percent_Identity=31.9884726224784, Blast_Score=177, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6321399, Length=358, Percent_Identity=26.536312849162, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324421, Length=360, Percent_Identity=28.6111111111111, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321281, Length=360, Percent_Identity=25.8333333333333, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320750, Length=366, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38132; Mature: 38001

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTNKKLILVTGGSGYIAMFIMIALLKKGYRVRATLRMMSRQEEVKKMMKQGGIADFTD
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LDFVQTDLTKEEGWSQAMTGVDSVIHVASPTPLQRPDADDLMVIMAVDGVKFVMRAAKEA
CHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHC
GVKRVVLTSASGAVLAGHKHHPEIFTEKDWTKLDAPINAYQRSKTMAELEFWKLAKSYGM
CCEEEEEECCCCCEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EGASVLPTAVMGPILGNDFSHSSASIKNMFEGKMPRLLNLAFDYVDVRDVADLHLLSLEK
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
EEAISQRFIATSGQNITYQEQAKFLKKEFGSKAKKVSTQVIPDFLVKILARFDKSLAMPA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TFLGQNTACSNEKSTRLLGWQPRSGEAAIIQTAQTMLDLAVIKVD
HHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHEEEEEEC
>Mature Secondary Structure 
TNTNKKLILVTGGSGYIAMFIMIALLKKGYRVRATLRMMSRQEEVKKMMKQGGIADFTD
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LDFVQTDLTKEEGWSQAMTGVDSVIHVASPTPLQRPDADDLMVIMAVDGVKFVMRAAKEA
CHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHC
GVKRVVLTSASGAVLAGHKHHPEIFTEKDWTKLDAPINAYQRSKTMAELEFWKLAKSYGM
CCEEEEEECCCCCEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EGASVLPTAVMGPILGNDFSHSSASIKNMFEGKMPRLLNLAFDYVDVRDVADLHLLSLEK
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCH
EEAISQRFIATSGQNITYQEQAKFLKKEFGSKAKKVSTQVIPDFLVKILARFDKSLAMPA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TFLGQNTACSNEKSTRLLGWQPRSGEAAIIQTAQTMLDLAVIKVD
HHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]