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The map label for this gene is purF [H]

Identifier: 125623816

GI number: 125623816

Start: 941006

End: 942526

Strand: Direct

Name: purF [H]

Synonym: llmg_0977

Alternate gene names: 125623816

Gene position: 941006-942526 (Clockwise)

Preceding gene: 125623815

Following gene: 125623817

Centisome position: 37.2

GC content: 44.51

Gene sequence:

>1521_bases
ATGTCTGATGAAATGACAAGCGCAACATTTACTGAACAAGAGTGGTCGCATTTTGAGAAAAAAAGTTTGAATCAAACTGA
AGCCGATGAGCTCCGCGACTGGTCGCGGTTTGAAGCGAAGACATTAAATGAAGAATGTGGACTTTTCGGCGTTTGGGGAC
ATCCTGATGCCGCTCGTCTAACCTATTTTGGTCTTCACGCTTTGCAACATCGCGGGCAAGAAGGCGCAGGAATTCTGGTC
AATAACAACGGCAAGCTCAATCGTCATCGTGGTTTAGGTTTGGTCACCGAAGTTTTTCGTCACGAAAAAGATTTGGAAGA
ACTGACAGGTTCATCTGCCATTGGTCATGTCCGCTATGCGACGGCTGGCTCGGCAAATATTAATAATATTCAGCCATTCC
AGTTTGAATTTCATGACGGATCACTTGGACTTGCCCACAATGGAAATTTAACTAACGCTCAATCCTTGCGCTGTGAACTT
GAAAAATCTGGAGCAATATTTTCTAGTAATTCAGACACAGAAATCCTCATGCACTTGATTCGCCGTAGTCATCATCCAGA
ATTTATGGGCAGAGTCAAAGAAGCACTTAATACGGTCAAAGGTGGCTTTGCCTATCTTATTATGACTGAAAATTCAATTG
TCGCAGCTCTTGACCCTAATGGATTTCGGCCACTTTCAATCGGTAAAATGAGCAACGGTGCGCTTGTCGTGGCCTCAGAA
ACTTGTGCTTTCGATGTTGTTGGCGCCACTTGGATTCAAGATGTTCAACCCGGTGAAATCATTGAAATTAATGACGACGG
CATTCATGTGGACCAATTTACTGACAGCACCAACATGACTATTTGTTCAATGGAATACATCTATTTCGCTCGTCCTGACA
GCAATATCGCTGGAGTTAATGTTCATACTGCCAGAAAACGTTCAGGAAAAATTTTAGCCCAAGAAGCCAAAATTGACGCT
GATATTGTCATTGGTGTCCCTAATTCATCACTCAGTGCCGCTTCAGGCTACGCTGAAGAATCGGGCTTGCCTTACGAAAT
GGGGCTGATTAAAAATCAATATGTGGCAAGAACTTTCATTCAACCGACTCAAGAACTTCGTGAACAAGGCGTTCGCATGA
AATTGAGTGCTGTTCGAGGTGTTGTAGAAGGAAAACGTGTCATCATGGTTGATGACAGCATTGTGCGTGGAACGACCAGC
CGTCGCATTGTCAAACTCCTCAAAGATGCAGGCGCAGCCGAGGTTCATGTGGCAATCGCCAGTCCCGCTCTCAAATATCC
TTGCTTTTACGGCATTGACATCCAAGATCGTGACGAACTCATCGCAGCCACACACACGACTGATGAAATCAGAGAAGCTA
TCGGAGCGGATAGTCTGACCTATCTCAGCCAGTCAGGGCTTGTCGAGGCGATCGGTCACGATAAACTTTGCTTGTCATAT
TTTGACGGCGAATATCCAACACCGCTTTATGATTATGAAGCAGACTATTTGGAATCTTTGGCAAAAAATGGTCTGAAATA
A

Upstream 100 bases:

>100_bases
GCGAAGTCATCGGAAAAGTTACTGACAACGGAATTTTGAAAATTAATGAACTTTCTATTTCTACTGACGAAGCTGTCAGC
ATTTATGAAGGAGCCCTTCC

Downstream 100 bases:

>100_bases
AAACTAGAGTATTTTCTCTAGTTTTTAATTTGGTCAAATCCATAAAGCGATTATTTTTTATTAGTTTCCTTAATATGTTA
GGATAAGAAATAAAAGCAAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSDEMTSATFTEQEWSHFEKKSLNQTEADELRDWSRFEAKTLNEECGLFGVWGHPDAARLTYFGLHALQHRGQEGAGILV
NNNGKLNRHRGLGLVTEVFRHEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGSLGLAHNGNLTNAQSLRCEL
EKSGAIFSSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASE
TCAFDVVGATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAKIDA
DIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTS
RRIVKLLKDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQSGLVEAIGHDKLCLSY
FDGEYPTPLYDYEADYLESLAKNGLK

Sequences:

>Translated_506_residues
MSDEMTSATFTEQEWSHFEKKSLNQTEADELRDWSRFEAKTLNEECGLFGVWGHPDAARLTYFGLHALQHRGQEGAGILV
NNNGKLNRHRGLGLVTEVFRHEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGSLGLAHNGNLTNAQSLRCEL
EKSGAIFSSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASE
TCAFDVVGATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAKIDA
DIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTS
RRIVKLLKDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQSGLVEAIGHDKLCLSY
FDGEYPTPLYDYEADYLESLAKNGLK
>Mature_505_residues
SDEMTSATFTEQEWSHFEKKSLNQTEADELRDWSRFEAKTLNEECGLFGVWGHPDAARLTYFGLHALQHRGQEGAGILVN
NNGKLNRHRGLGLVTEVFRHEKDLEELTGSSAIGHVRYATAGSANINNIQPFQFEFHDGSLGLAHNGNLTNAQSLRCELE
KSGAIFSSNSDTEILMHLIRRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASET
CAFDVVGATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVNVHTARKRSGKILAQEAKIDAD
IVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFIQPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSR
RIVKLLKDAGAAEVHVAIASPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQSGLVEAIGHDKLCLSYF
DGEYPTPLYDYEADYLESLAKNGLK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=468, Percent_Identity=41.025641025641, Blast_Score=342, Evalue=3e-94,
Organism=Escherichia coli, GI1788651, Length=489, Percent_Identity=38.241308793456, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790167, Length=228, Percent_Identity=28.9473684210526, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=40.3726708074534, Blast_Score=325, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6323958, Length=478, Percent_Identity=34.7280334728033, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=41.0204081632653, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=42.3326133909287, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=40.6374501992032, Blast_Score=351, Evalue=8e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55671; Mature: 55540

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEMTSATFTEQEWSHFEKKSLNQTEADELRDWSRFEAKTLNEECGLFGVWGHPDAARL
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
TYFGLHALQHRGQEGAGILVNNNGKLNRHRGLGLVTEVFRHEKDLEELTGSSAIGHVRYA
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TAGSANINNIQPFQFEFHDGSLGLAHNGNLTNAQSLRCELEKSGAIFSSNSDTEILMHLI
ECCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHEEEECCCCCEEECCCCHHHHHHHH
RRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASE
HHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCEEEEEEC
TCAFDVVGATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVN
CCEEEECCHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEEEEECCCCCEEEEE
VHTARKRSGKILAQEAKIDADIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFI
EECCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHH
QPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLLKDAGAAEVHVAIA
HHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEC
SPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQSGLVEAIGHDKLCLSY
CCCCCCCEEEECCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHCCCHHHCCCCCEEEEE
FDGEYPTPLYDYEADYLESLAKNGLK
CCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SDEMTSATFTEQEWSHFEKKSLNQTEADELRDWSRFEAKTLNEECGLFGVWGHPDAARL
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
TYFGLHALQHRGQEGAGILVNNNGKLNRHRGLGLVTEVFRHEKDLEELTGSSAIGHVRYA
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEE
TAGSANINNIQPFQFEFHDGSLGLAHNGNLTNAQSLRCELEKSGAIFSSNSDTEILMHLI
ECCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHEEEECCCCCEEECCCCHHHHHHHH
RRSHHPEFMGRVKEALNTVKGGFAYLIMTENSIVAALDPNGFRPLSIGKMSNGALVVASE
HHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCEEEEEEC
TCAFDVVGATWIQDVQPGEIIEINDDGIHVDQFTDSTNMTICSMEYIYFARPDSNIAGVN
CCEEEECCHHHHCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEEEEECCCCCEEEEE
VHTARKRSGKILAQEAKIDADIVIGVPNSSLSAASGYAEESGLPYEMGLIKNQYVARTFI
EECCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHCCCCEEHHHHHHHHHHHHHH
QPTQELREQGVRMKLSAVRGVVEGKRVIMVDDSIVRGTTSRRIVKLLKDAGAAEVHVAIA
HHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEC
SPALKYPCFYGIDIQDRDELIAATHTTDEIREAIGADSLTYLSQSGLVEAIGHDKLCLSY
CCCCCCCEEEECCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHCCCHHHCCCCCEEEEE
FDGEYPTPLYDYEADYLESLAKNGLK
CCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA