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The map label for this gene is lmrB [H]

Identifier: 125623806

GI number: 125623806

Start: 932929

End: 934302

Strand: Direct

Name: lmrB [H]

Synonym: llmg_0967

Alternate gene names: 125623806

Gene position: 932929-934302 (Clockwise)

Preceding gene: 125623805

Following gene: 125623807

Centisome position: 36.88

GC content: 37.34

Gene sequence:

>1374_bases
ATGAATAGAGAATTAGAAAAAAATAAAGCGAATCATAAAATTAGCGCGAAAACGCGCTTTTCGATTGTCGCTGTGGCTCT
TGTGGCCTTTATGGGGATATTGGCGGAAACAAGTCTGAATGTGACTTTCCCCACGCTTGAGAAAACTTTTGGACTTCCAC
TAAGTACGGTTCAATGGGTGACGACAGCTTATTTGCTTGTTGTTGCTATTGTTATGCCTACAAGTTCATACATCAGCCAA
CGTTTTCAAAACCGAACCTTGTTTTTTGCAGGATTAATCTTCTTTACTATCGGTGATTTATTGAGTTTAATTTCATCAAA
TTTTACTATTTTGATGGCTGGACGTTTGATTCAAGGGATTGGGACTGGGATTGCAATTCCTTTAATGTTTAATCTTGTCT
TGACTAAAATTCCTCGTGAACGGGTTGGTCTTTGGATGGGATTTGCTGGTATGATTTTGAGTATTGCACCCGCACTTGGC
CCAACTTATGGGGGGCTTTTAACGGGAACTCTTGGCTGGCGAATGATTTTTGCAATTATTTTAATTATCCCAATCTGCTT
ATTTCCTTTGGGAGCTTGGGCTATTGAAAAGAGTGAAAAAACTCGTCCAGGTAAATTTGATTTTATCGCTTTTATTTTCT
TATCACTTGTGATGACCAGTGCCCTTTTAGCTTTAGATTCATTATCAAGTGCTCATTTTTCATGGATTTGGTTAATCCTC
TTTATTATTGGATTAATTGCCTTTATTTGGCGTTCAAAAACAAGTTCTTTGAATTTTATAGATATCAATGTCTTTAAGAG
TCCAACCTTTACTTTGTCAGTCCTTGTTTATTGTATTTTACAATTTGTAAATTTGGGTATTAACTTTATCATCCCTAATT
TCTTACAGGTTTCTCTTGGTGCCACAAGTACCTTGGCAGGTTTTGCTTTATTACCTGGTTCATTAATTGGTTCAGCGGCG
CAAGGACCAATCGGTGCACTTTATGACCGAAAAGGGGCCAAGGGTATCTTATATAGCGGGAACACTGTTTTCTTCATTGC
ACTTGTATTGTTAACGATTTTTACTAAAAATCTAACTTTACTGGGTGTGATGGGACTTTATGTTGTATTCACAATCGGAC
GTTCGTCAAGCTTTGCAACGACAATGACCAATAGTCTAGCTCAACTCCCGCATGATAAAGCCGCTTCTGGAAATGCTTGG
TTTACTACGACTTCTCAATTTGCGGCATCAGCAGGGACGGCGACGGCCAGTCTGATTGCAACAAAATCAAGTAATTTAAC
AACCGGAACTCAACATGTATTTATGTTTTTCCTTTGTCTTGCTTTACTTAACTTTGTTTTCTATACTTTGATTTTGAATA
AAAAAATAAAATAA

Upstream 100 bases:

>100_bases
TCCAAGCACATAATAAAAAAGCTTTGACAGGATTATCAACAGAAGAGATTGCTGCAGTTGAAAAATTTCTTAATAATTTT
AGCGTGCAGGAAGGAAGAGA

Downstream 100 bases:

>100_bases
ATTTTGATGAAAGTTTCGTAATTATACGAAGCTTTTTTATAATTTAATATAGTATGCTTAAAAAGCATAATTATTGATAT
AAAAAGCATTTCACGTCAGT

Product: putative permease protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNRELEKNKANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFAGLIFFTIGDLLSLISSNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKTRPGKFDFIAFIFLSLVMTSALLALDSLSSAHFSWIWLIL
FIIGLIAFIWRSKTSSLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGATSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK

Sequences:

>Translated_457_residues
MNRELEKNKANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFAGLIFFTIGDLLSLISSNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKTRPGKFDFIAFIFLSLVMTSALLALDSLSSAHFSWIWLIL
FIIGLIAFIWRSKTSSLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGATSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
>Mature_457_residues
MNRELEKNKANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWVTTAYLLVVAIVMPTSSYISQ
RFQNRTLFFAGLIFFTIGDLLSLISSNFTILMAGRLIQGIGTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALG
PTYGGLLTGTLGWRMIFAIILIIPICLFPLGAWAIEKSEKTRPGKFDFIAFIFLSLVMTSALLALDSLSSAHFSWIWLIL
FIIGLIAFIWRSKTSSLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLGATSTLAGFALLPGSLIGSAA
QGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTLLGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAW
FTTTSQFAASAGTATASLIATKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=25.41567695962, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788392, Length=448, Percent_Identity=26.3392857142857, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788710, Length=303, Percent_Identity=28.0528052805281, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789042, Length=312, Percent_Identity=27.2435897435897, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=22.9665071770335, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=286, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49647; Mature: 49647

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELEKNKANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWV
CCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCHHHHHHH
TTAYLLVVAIVMPTSSYISQRFQNRTLFFAGLIFFTIGDLLSLISSNFTILMAGRLIQGI
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALGPTYGGLLTGTLGWRMIFAII
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIPICLFPLGAWAIEKSEKTRPGKFDFIAFIFLSLVMTSALLALDSLSSAHFSWIWLIL
HHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FIIGLIAFIWRSKTSSLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLG
HHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCC
ATSTLAGFALLPGSLIGSAAQGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTL
CHHHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAWFTTTSQFAASAGTATASLIA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHEE
TKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRELEKNKANHKISAKTRFSIVAVALVAFMGILAETSLNVTFPTLEKTFGLPLSTVQWV
CCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCHHHHHHH
TTAYLLVVAIVMPTSSYISQRFQNRTLFFAGLIFFTIGDLLSLISSNFTILMAGRLIQGI
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GTGIAIPLMFNLVLTKIPRERVGLWMGFAGMILSIAPALGPTYGGLLTGTLGWRMIFAII
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIPICLFPLGAWAIEKSEKTRPGKFDFIAFIFLSLVMTSALLALDSLSSAHFSWIWLIL
HHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FIIGLIAFIWRSKTSSLNFIDINVFKSPTFTLSVLVYCILQFVNLGINFIIPNFLQVSLG
HHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHCC
ATSTLAGFALLPGSLIGSAAQGPIGALYDRKGAKGILYSGNTVFFIALVLLTIFTKNLTL
CHHHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LGVMGLYVVFTIGRSSSFATTMTNSLAQLPHDKAASGNAWFTTTSQFAASAGTATASLIA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCCHHHHHEE
TKSSNLTTGTQHVFMFFLCLALLNFVFYTLILNKKIK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]